电子垃圾拆解区育龄夫妇不良妊娠的遗传学分析

发布时间：2018-11-09 17:22

【摘要】： 目的:本研究通过分析长期居住在电子垃圾处理场区域并出现不良妊娠(滞留流产、胎儿畸形、死胎)情况的年轻夫妇的染色体畸变和微核的情况,结合单细胞凝胶电泳实验和相关微量元素的检测,探讨该人群遗传物质损伤的程度及电子垃圾污染物对当地人群生殖功能的影响。 方法:选取20对长期居住在电子垃圾处理场区域,居住半径≤5公里,已经出现不良妊娠情况的夫妻作为研究组,对照组为未接触污染物并且居住地相距20～50公里的40位居民(男、女各20位),条件与研究组相匹配。取其外周血进行染色体核型分析、微核检测、单细胞凝胶电泳实验以及微量元素(铜、铁、镁、锌、铅、硒)的观察,并与的对照组比较,用t检验(染色体畸变率、TDNA%、TM、OTM及各种微量元素含量)和x~2检验(微核率)进行统计学分析。 结果:(1)研究组染色体畸变率无着丝粒断片发生率:0.1%±0.003;微小体的发生率:0.225%±0.005;双着丝粒+环状染色体:0.175%±0.004;单体断裂:0.15%±0.004;单体裂隙:0.075%±0.003,与对照组比较,差异有统计学意义(P0.01)。(2)研究组微核率为13.63‰,对照组微核率为3.47‰,差异有统计学意义(P0.01)。研究组中女性的微核率为12.4‰,低于同组中男性微核率(14.85‰),差异有统计学意义。(3)单细胞凝胶电泳实验检测分析研究组慧星尾部DNA百分含量(TDNA%):6.4107%±3.53743;尾矩(TM):1.5883%±1.36788;Olive尾矩(OTM):1.9329%±1.03982,与对照组比较差异均有统计学意义(P0.01)。(4)研究组血铅含量为99.59±18.29μg/l,对照组为37.88±7.36μg/l;研究组血锌含量为75.51±12.87μg/l;对照组为101.06±23.79μg/l;研究组血铁含量为7.24±0.97μg/l,对照组为8.34±1.71μg/l,研究组血镁含量为1.61±0.18μg/l,对照组为1.45±0.13μg/l,差异均有统计学意义(P0.01)。血铜和血硒含量比较,差异无统计学意义(P0.05)。 结论:研究结果提示,不科学的电子垃圾处理产生的污染物是该地区人群潜在的遗传诱变剂,造成遗传物质损伤,进而影响生殖功能,严重时会导致不良奸娠。
[Abstract]:Objective: to study the chromosomal aberrations and micronuclei of young couples who have been living in the electronic waste disposal site for a long time and have adverse pregnancy (residual abortion, fetal malformation, stillbirth). Combined with single cell gel electrophoresis (SCGE) and the detection of related trace elements, the degree of genetic material damage and the effect of electronic garbage pollutants on the reproductive function of local population were studied. Methods: a total of 20 couples with a living radius of less than 5 km and who had already had adverse pregnancy were selected as the study group. The control group consisted of 40 residents (20 males and 20 females) who were not exposed to contaminants and lived 2050 km away from each other. The conditions were matched with the study group. Chromosome karyotype analysis, micronucleus detection, single cell gel electrophoresis (SCGE) and trace elements (copper, iron, magnesium, zinc, lead, selenium) in peripheral blood were analyzed and compared with control group. T test (chromosome aberration rate, TDNA%,) was used. TM,OTM and various trace elements) and xan2 test (micronucleus rate) were analyzed statistically. Results: (1) the rate of chromosome aberration in study group was 0.1% 卤0.003, 0.225% 卤0.005 for microsomes, 0.175% 卤0.004 for bicentric chromosomes and 0.175% 卤0.004 for bicentric chromosomes. Monomer fracture: 0.15% 卤0.004; The monomeric fissure: 0.075% 卤0.003, the difference was statistically significant (P0.01). (2), the micronucleus rate in the study group was 13.63 鈥，

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