骨髓间充质干细胞的磁标记成像及移植治疗骨缺血坏死的研究

发布时间：2018-11-11 16:55

【摘要】： 股骨头缺血性坏死是临床常见病、多发病,治疗上除手术外都是基于改善股骨头周围血液循环。骨髓间充质干细胞取材方便,能在体外大量扩增,具有多种分化潜能,来源于自体骨髓的细胞移植时不存在免疫排斥,同种异体的间充质干细胞免疫原性很低,为组织损伤修复提供了新的种子细胞。 本研究采用密度梯度离心分离法对rMSCs进行分离扩增,通过形态学和成骨、成脂方向诱导分化,证实分离得到的细胞为骨髓间充质干细胞。采用菲立磁-多聚赖氨酸对得到的rMSCs进行磁标记,标记率为100%;用1.5TMRI对标记细胞进行了体外成像研究,表明GRE T2*WI序列可以作为磁标记细胞研究的首选序列。通过注射马血清及甲泼尼龙的方法建立了兔激素性股骨头缺血坏死模型,该模型骨坏死处于0～I期,是研究早期骨坏死的理想模型。采用介入导管技术将骨髓间充质干细胞移植到兔股骨头缺血坏死模型体内,显示移植后病变部位可见明显骨修复,移植细胞可以在病变局部存活。在此基础上采用全骨髓培养法对胎儿fMSCs进行分离和扩增,并进行fMSCs磁标记成像,获得了与兔MSCs相似的结果。本研究从兔骨髓间充质干细胞研究获得经验并逐步应用到人骨髓间充质干细胞研究上,期望为进一步的骨髓间充质干细胞移植治疗股骨头缺血坏死的临床应用做些有益的探索。
[Abstract]:Avascular necrosis of the femoral head is a common clinical disease. The treatment of avascular necrosis of femoral head is based on the improvement of peripheral blood circulation except surgery. Bone marrow mesenchymal stem cells (BMSCs) are easy to take, can be expanded in vitro, and have a variety of differentiation potential. There is no immune rejection in transplantation of cells derived from autologous bone marrow, and the immunogenicity of allogeneic mesenchymal stem cells is very low. It provides a new seed cell for tissue damage repair. In this study, rMSCs was isolated and amplified by density gradient centrifugation. The cells were identified as bone marrow mesenchymal stem cells (BMSCs) by morphology and osteogenesis. The labeled rMSCs was labeled with phenidomagnetic poly-lysine and the labeling rate was 100. The in vitro imaging of labeled cells with 1.5TMRI showed that the GRE T2*WI sequence could be the preferred sequence in the study of magnetically labeled cells. A rabbit model of steroid-induced avascular necrosis of femoral head was established by injecting horse serum and methylprednisolone. Bone marrow mesenchymal stem cells (BMSCs) were transplanted into rabbit model of avascular necrosis of femoral head by interventional catheter technique. On this basis, fetal fMSCs was isolated and amplified by whole bone marrow culture method, and fMSCs magnetic labeling imaging was performed. The results were similar to those of rabbit MSCs. In this study, we obtained experience from rabbit bone marrow mesenchymal stem cell research and applied it to human bone marrow mesenchymal stem cell research step by step, hoping to make some useful exploration for the further clinical application of bone marrow mesenchymal stem cell transplantation in the treatment of avascular necrosis of femoral head.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2009
【分类号】：R329

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