糖尿病大鼠模型的建立及相关蛋白组学的实验研究

发布时间：2018-11-13 11:09

【摘要】：目的:研究糖尿病大鼠模型的建立及其蛋白组学的变化,从蛋白质的分子水平上探索糖尿病发生的病理生理机制。方法:用高脂高糖饮食+链脲佐菌素的方法建立糖尿病大鼠模型,采用同位素标记技术,利用高效液相色谱仪及四级杆-静电场轨道阱质谱仪,对糖尿病大鼠进行串级质谱数据分析,对检测出的有统计学意义的表达差异蛋白进行研究。结果:第2周开始,模型组与正常组的血糖差异有统计学意义(P0.05)。第10周,模型组血糖基本达到了糖尿病模型的标准,空腹血糖16.7 mmol/L;蛋白组学检测共获得有显著差异的蛋白50个,其中上调大于1.5倍的蛋白17个,主要有MHCⅡ反式激活因子、主要尿蛋白、LIM结构域蛋白等;下调小于0.75倍的50个,主要有肉碱乙酰转移酶、多聚免疫球蛋白受体前体等。结论:建立了相对符合糖尿病发生发展规律的糖尿病大鼠模型,血清蛋白组学分析糖尿病发生的机制中存在免疫系统、糖代谢、脂代谢紊乱,从蛋白分子的水平研究糖尿病发生的病理生理基础,具有重要学术和临床意义。
[Abstract]:Aim: to study the establishment of diabetic rat model and its proteomic changes, and to explore the pathophysiological mechanism of diabetes mellitus at the molecular level. Methods: the diabetic rat model was established by using high fat and high sugar diet streptozotocin. High performance liquid chromatography (HPLC) and quaternary bar electrostatic field trap mass spectrometer were used. The differentially expressed proteins in diabetic rats were analyzed by cascade mass spectrometry. Results: there was significant difference in blood glucose between the model group and the normal group at the second week (P0.05). In the 10th week, the blood glucose of the model group basically reached the standard of diabetic model, fasting blood glucose 16. 7 mmol/L; A total of 50 proteins with significant difference were obtained by proteomics analysis, 17 of which were up-regulated more than 1.5 times, mainly MHC 鈪，

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