外周血淋巴细胞微核标本制片室内质量控制

发布时间：2018-11-17 20:49

【摘要】：目的了解外周血淋巴细胞微核标本制片的影响因素,寻找出最佳的室内质量控制制作条件。方法比较制片过程中不同的接种血量、培养温度、低渗时间以及离心速度对外周血中微核标本制作的影响,寻找最佳的室内质量控制制片效果。结果外周血微核制片过程中最佳的室内质量控制条件是接种血量是0.7×32规格注射器针头20~25滴;培养温度应该严格控制在(37.0±0.5)℃;最佳低渗时间是6~9min;最佳离心速度1500~2000 r/min;固定液应在使用前临时配置,放置1 h应废弃;玻片冷藏处理的效果比热水处理后的较好。结论外周血淋巴细胞微核标本制片的影响因素有接种血量、培养温度、低渗时间以及离心速度等,相关实验室予以关注,以确保其制备质量,提高微核检出准确率和读片效率,及时准确反映辐射损伤情况,对辐射防护作出正确的评价。
[Abstract]:Objective to investigate the influencing factors of micronucleus preparation in peripheral blood lymphocytes and to find out the best indoor quality control conditions. Methods the effects of different inoculation blood volume, culture temperature, low osmotic time and centrifugation speed on the preparation of micronucleus samples in peripheral blood were compared in order to find out the best effect of indoor quality control. Results the optimal indoor quality control conditions during the preparation of peripheral blood micronucleus were as follows: blood volume of 0.7 脳 32 standard syringe needle 2025 drops, culture temperature strictly controlled at (37.0 卤0.5) 鈩，

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