豚鼠左心室流出道慢反应自律细胞分布区域的形态结构
发布时间：2018-11-21 09:05
【摘要】： 心室流出道(左室的主动脉前庭和右室的动脉圆锥),一向被认为仅是血液的输出路径,因而对其形态学和生理学特点一直缺乏详细研究。我室近年来研究发现,在豚鼠、兔及大鼠的主动脉前庭的特定部位可记录到自律性慢反应动作电位,表明在这一部位存在慢反应自律细胞。这是在传统的资料中未见记载的细胞的存在,提示心室流出道可能在心脏活动过程中具有一定未知的功能。但以往的研究多是对这种电位的性质、离子流基础等方面的分析,而对产生这种电位的形态学基础尚未见进一步研究。为探讨心室流出道组织的结构特点和神经分布,开展了本项工作。 在本实验中,利用常规细胞Qg玻璃微电极技术,引导和分析豚鼠左心室流出道自发慢反应电位,并在此基础上,确定慢反应自律细胞的分布区域,并将此区域制成形态学标本,采用HE、Masson染色,镀银染色和透射电镜技术,对该区域的组织标本,进行了光镜和电镜观察。重点是观察该区域细胞的结构,胶原纤维、网状纤维的分布,以确定慢反应自律细胞分布的确切部位及这种细胞的超微结构。为进一ki从形态学探讨这种自律细胞是否受自主神经的调控,采用不同浓度的肾上腺素能和乙酰胆碱能激动剂对该部位自发慢反应电位影响,在此基础上,通过免疫组织化学染色,观察了神经肽Y(neuropeptideY,NPY)能神经纤维的分布,以判断该区域是否接受自主神经纤维的支配。 在大部分左心室流出道的标本上可记录到自发慢反应电位,经对这些标本的形态学观察发现,在主动脉瓣后瓣根部和心室肌的交界处,有一类似于月牙形的淡染区域。该区域细胞密集,可成团或单独存在,细胞形态类似P细胞样细胞,可能就是慢反应自律细胞的存在部位。细胞团周围含有丰富的胶原纤维和网状纤维,对细胞团的自律性电活动可能起着绝缘和保护作用。 用交感神经递质去甲肾上腺素(norepinephrine ,NE)和肾上腺素(epinephrine,E)灌流时,可使自发放电频率(rate of pacemakerfiring ,RPF),动作电位幅值(amplitude of aetion potential,APA),4相自动去极速率(veloeity of diastolic depolarization,VDD),0相最大除极速率(maximal rate of depolarization,Vmax)和最大舒张电位(maximal diastolic potential,MDP)明显增加,但复极50%时间(50%of duration of action potential,APD50)和复极90%时间(90% of durationof action potential,APD90)变化不明显,改用正常液冲洗后,上述指标逐渐恢复。用副交感神经递质(ACh)灌流时,可使RPF,APA,VDD,Vmax和MDP绝对值明显下降,APD50和APD90增加明显,改用正常液冲洗后,上述指标有不同程度的恢复。这些结果表明,自主神经末稍的递质对该部慢反应自律细胞的活动有明显作用。利用免疫组织化学染色观察发现,在包绕细胞团周围的结缔组织中可见大量的NPY能阳性神经纤维呈线状或束状分布,在细胞团内也可偶见NPY阳性神经纤维。根据NPY是与NE共存于交感神经末稍的递质,表明产生慢反应电位的细胞团可能接受交感神经纤维的调控。 在此实验中,我们发现心室流出道存在一特殊的细胞团结构,该细胞团可能是产生慢反应动作电位的自律细胞的存在部位;NPY能阳性神经纤维的存在,提示这些部位可能接受交感神经的支配。
[Abstract]:The ventricular outflow tract (the arterial cone of the aortic vestibule and the right ventricle of the left chamber), which has been considered to be only the output path of the blood, has been lacking in a detailed study of its morphology and physiological characteristics. In recent years, I have found that a specific site in the aortic vestibule of guinea pigs, rabbits and rats can be recorded to a self-disciplined slow response action potential, indicating a slow response to self-regulation at this site. This is the absence of the cell described in the conventional data suggesting that the ventricular outflow tract may have an unknown function during cardiac activity. However, the previous studies have been made on the analysis of the properties of such potential and the basis of ion current, and the morphological basis for this potential has not yet been further studied. In order to study the structure and nerve distribution of the ventricular outflow tract, this work was carried out. In this experiment, the spontaneous slow reaction potential of the left ventricular outflow tract of the guinea pig was guided and analyzed by using the conventional cell Qg glass microelectrode technique, and on this basis, the distribution area of the slow-response self-regulation cell was determined, and the region was made into a morphological specimen, and HE and Mass were used. On the basis of the technique of dyeing, silver-plating and transmission electron microscopy, the tissue specimens of the region were examined and the light microscope and the light microscope were carried out. The focal point was to observe the structure of the cell, the distribution of the collagen fibers and the reticulation fibers to determine the exact location of the slow response self-regulation cell distribution and the cells. The ultrastructure of neuropeptide Y (neuroppt) was observed by immunohistochemical staining. ideY, NPY) the distribution of the nerve fibers to determine if the region is receptive to the autonomic fiber The control of the dimension. The spontaneous slow response potential can be recorded on the majority of the left ventricular outflow tract, and the morphology of these specimens has been found to be similar at the junction of the root of the aortic valve and the ventricular muscle. in a crescent-shaped light-dyed region. The region is dense, can be clustered or alone, and the cell morphology is similar to that of a P-cell-like cell, which may be a slow response. The presence of self-regulatory cells. There are abundant collagen fibers and reticular fibers around the cell mass, and the self-regulatory activity of the cell group may The spontaneous discharge frequency (RPF), the action potential amplitude value (APA), and the 4-phase autopolar rate (velocity of distaolic) can be achieved with the perfusion of the sympathomimetic norepinephrine (NE) and the epinephrine (E). The maximum depolarization rate (Vmax) and the maximum diastolic potential (MDP) of the 0-phase were significantly increased, but the time of 50% time (50% of duration of action, APD 50) and the time of the complex electrode (90% of time of action, APPD90) the change is not obvious, and the change is used instead The indexes of RPF, APA, VDD, Vmax, and MDP were significantly reduced after the normal fluid was flushed. The absolute values of RPF, APA, VDD, Vmax and MDP were significantly decreased with the perfusion of the parasympathetic nerve (ACh), and the APD50 and APPD90 increased significantly, and the normal liquid was used for flushing. The indicators showed a different degree of recovery, and the results showed that the end of the autonomic nerve was slower than that of the Department. It is found that a large number of NPY-positive nerve fibers in the connective tissue around the cell mass are linear or bundle-like in the connective tissue surrounding the cell mass, NPY-positive nerve fibers can also be seen occasionally. According to NPY, the cells that co-exist with the NE at the end of the sympathetic nerve indicate that the cells that produce the slow response potential It is possible to control the sympathetic fiber. In this experiment, we found that there was a special cell mass structure in the ventricular outflow tract, which could be the site of self-regulation of the slow response action potential, and the existence of NPY-positive nerve fibers.
【学位授予单位】：河北北方学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R322.11
本文编号：2346512
[Abstract]:The ventricular outflow tract (the arterial cone of the aortic vestibule and the right ventricle of the left chamber), which has been considered to be only the output path of the blood, has been lacking in a detailed study of its morphology and physiological characteristics. In recent years, I have found that a specific site in the aortic vestibule of guinea pigs, rabbits and rats can be recorded to a self-disciplined slow response action potential, indicating a slow response to self-regulation at this site. This is the absence of the cell described in the conventional data suggesting that the ventricular outflow tract may have an unknown function during cardiac activity. However, the previous studies have been made on the analysis of the properties of such potential and the basis of ion current, and the morphological basis for this potential has not yet been further studied. In order to study the structure and nerve distribution of the ventricular outflow tract, this work was carried out. In this experiment, the spontaneous slow reaction potential of the left ventricular outflow tract of the guinea pig was guided and analyzed by using the conventional cell Qg glass microelectrode technique, and on this basis, the distribution area of the slow-response self-regulation cell was determined, and the region was made into a morphological specimen, and HE and Mass were used. On the basis of the technique of dyeing, silver-plating and transmission electron microscopy, the tissue specimens of the region were examined and the light microscope and the light microscope were carried out. The focal point was to observe the structure of the cell, the distribution of the collagen fibers and the reticulation fibers to determine the exact location of the slow response self-regulation cell distribution and the cells. The ultrastructure of neuropeptide Y (neuroppt) was observed by immunohistochemical staining. ideY, NPY) the distribution of the nerve fibers to determine if the region is receptive to the autonomic fiber The control of the dimension. The spontaneous slow response potential can be recorded on the majority of the left ventricular outflow tract, and the morphology of these specimens has been found to be similar at the junction of the root of the aortic valve and the ventricular muscle. in a crescent-shaped light-dyed region. The region is dense, can be clustered or alone, and the cell morphology is similar to that of a P-cell-like cell, which may be a slow response. The presence of self-regulatory cells. There are abundant collagen fibers and reticular fibers around the cell mass, and the self-regulatory activity of the cell group may The spontaneous discharge frequency (RPF), the action potential amplitude value (APA), and the 4-phase autopolar rate (velocity of distaolic) can be achieved with the perfusion of the sympathomimetic norepinephrine (NE) and the epinephrine (E). The maximum depolarization rate (Vmax) and the maximum diastolic potential (MDP) of the 0-phase were significantly increased, but the time of 50% time (50% of duration of action, APD 50) and the time of the complex electrode (90% of time of action, APPD90) the change is not obvious, and the change is used instead The indexes of RPF, APA, VDD, Vmax, and MDP were significantly reduced after the normal fluid was flushed. The absolute values of RPF, APA, VDD, Vmax and MDP were significantly decreased with the perfusion of the parasympathetic nerve (ACh), and the APD50 and APPD90 increased significantly, and the normal liquid was used for flushing. The indicators showed a different degree of recovery, and the results showed that the end of the autonomic nerve was slower than that of the Department. It is found that a large number of NPY-positive nerve fibers in the connective tissue around the cell mass are linear or bundle-like in the connective tissue surrounding the cell mass, NPY-positive nerve fibers can also be seen occasionally. According to NPY, the cells that co-exist with the NE at the end of the sympathetic nerve indicate that the cells that produce the slow response potential It is possible to control the sympathetic fiber. In this experiment, we found that there was a special cell mass structure in the ventricular outflow tract, which could be the site of self-regulation of the slow response action potential, and the existence of NPY-positive nerve fibers.
【学位授予单位】：河北北方学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R322.11
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