内毒素血症大鼠心肌细胞骨架蛋白基因表达的变化

发布时间：2018-11-26 08:48

【摘要】：目的探讨内毒素血症大鼠心肌细胞骨架蛋白基因表达的变化。方法37只Wistar大鼠随机分两组,静脉注射精制内毒素或生理盐水,连续24h监测两组大鼠的心功能;另取54只大鼠注射精制内毒素之后不同时间点处死大鼠,取左心室心肌组织,给予常规病理切片、电子显微镜观察以及actin、tubulin、desmin等细胞骨架蛋白基因的转录水平检测。结果内毒素组大鼠LVSP在8～24h与对照组相比有显著降低,而LVEDP变化不明显,表明LPS以损害心肌收缩功能为主;显微结构实验显示给予内毒素处理不同时间段的大鼠心肌组织显微结构有明显的破坏,基因表达实验显示细胞骨架蛋白基因的转录水平变化随内毒素注射时间变化出现显著性差异。结论LPS可引起大鼠心功能及心肌细胞结构出现明显的损害,并引起心肌组织细胞骨架蛋白α-actin、tubulin基因表达发生显著的变化。
[Abstract]:Objective to investigate the changes of cytoskeleton gene expression in rat cardiomyocytes with endotoxemia. Methods Thirty-seven Wistar rats were randomly divided into two groups. After intravenous injection of refined endotoxin or normal saline, the cardiac function of the two groups was monitored for 24 hours. In addition, 54 rats were killed at different time points after injection of refined endotoxin, and left ventricular myocardial tissue was taken for routine pathological section, electron microscope observation and transcription level detection of cytoskeleton protein gene such as actin,tubulin,desmin. Results compared with the control group, the LVSP of the endotoxin group decreased significantly at 8h, but the change of LVEDP was not obvious, indicating that LPS mainly damaged myocardial contractile function. The results of microstructural test showed that the microstructures of myocardium of rats treated with endotoxin at different time periods were obviously damaged. Gene expression test showed that the transcriptional level of cytoskeleton gene varied significantly with the time of endotoxin injection. Conclusion LPS can cause significant damage to cardiac function and cardiomyocyte structure in rats, and change the expression of cytoskeleton 伪-actin,tubulin gene in myocardial tissue.
【作者单位】：南方医科大学医学基础实验教学中心;
【基金】：国家自然科学基金(30570707)
【分类号】：R363

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