机场噪声暴露对大鼠脑海马区神经元凋亡与Fas蛋白表达的影响
发布时间：2018-11-26 10:16
【摘要】： 目的 研究机场噪声暴露对大鼠脑海马区神经元凋亡与Fas蛋白表达的影响。为噪声污染影大鼠中枢神经系统的相关研究提供理论依据。 方法 采用浙江大学环境物理实验室噪声暴露后大鼠共16只作为实验模型。分为四组：正常饲养60天及90天的大鼠为对照组N1、N2,模拟萧山机场噪声暴露60天及脱离暴露后再正常饲养30天为实验组N3、N4。心脏灌注后行鼠脑切片,行Fas免疫组织化学染色、原位细胞凋亡检测(TUNEL法),用Image-Pro Plus6.0软件分析细胞阳性表达的积分光密度；另外再在透射电子显微镜下观察大鼠海马区神经元超微结构的改变。 结果 N1、N2组海马内Fas染色阳性表达的积分光密度分别为：2.67±1.67×103和3.03±2.18×103,两者比较无明显差异(P0.05)；N1、N2海马内TUNEL阳性表达的积分光密度分别为：44.10±1.67和51.75±10.90,两者比较亦无明显差异(P0.05)。N3、N4组海马内Fas染色阳性表达的积分光密度分别为：34.29±12.60×103和20.54±5.69×103,两者比较无明显差异(P0.05),但上述两组各自与N1、N2相比Fas染色阳性表达均显著增加(P0.05)；N3、N4海马内TUNEL阳性表达的积分光密度分别为：96.43±20.48和156.56±22.37,两者比较亦无明显差异(P0.05),N3、N4两组各自与N1、N2相比TUNEL阳性表达均显著增加(P0.05)。 透射电镜观察实验组海马区神经元胞核染色质浓聚,浓缩成块,列成沿核膜收缩的新月体,部分断裂,形成核碎片；细胞质内线粒体增大、空泡化,细胞骨架紊乱；细胞膜微绒毛、细胞突起及细胞间连接消失；出现凋亡小体；突触间隙模糊,染色质聚合,突触小泡不清晰。突触小泡减少,突触后膜肿胀,散在小血管周围胶质膜肿胀。 结论 长期噪声暴露可引起大鼠海马区神经元凋亡及Fas蛋白表达的增加。凋亡相关蛋白Fas表达的改变可能参与调控了细胞凋亡的过程。
[Abstract]:Objective to study the effects of airport noise exposure on neuronal apoptosis and Fas protein expression in rat hippocampal area. It provides a theoretical basis for the study of the central nervous system in rats exposed to noise pollution. Methods Sixteen rats were used as experimental models after noise exposure in the laboratory of environmental physics of Zhejiang University. The rats were divided into four groups: the control group was N1N _ 2 for 60 days and 90 days for normal feeding, and the experimental group was N3N _ 4 for 60 days of simulated noise exposure at Xiaoshan airport and 30 days of normal feeding for 30 days after detachment. After heart perfusion, brain sections were made, Fas immunohistochemical staining and in situ apoptosis assay (TUNEL) were performed. The integral optical density of positive cells was analyzed by Image-Pro Plus6.0 software. In addition, the ultrastructure of hippocampal neurons in rats was observed under transmission electron microscope. Results the integral optical density of Fas positive expression was 2.67 卤1.67 脳 10 ~ 3 and 3.03 卤2.18 脳 10 ~ 3 in N _ 1N _ 2 group, respectively. There was no significant difference between them (P0.05). The integral optical density of the positive expression of TUNEL in the hippocampus of N _ 1N _ 2 was 44.10 卤1.67 and 51.75 卤10.90, respectively, and there was no significant difference between the two groups (P0.05). The integral optical density of positive expression of Fas in hippocampus of N4 group was 34.29 卤12.60 脳 10 ~ 3 and 20.54 卤5.69 脳 10 ~ 3, respectively. There was no significant difference between the two groups (P0.05). The positive expression of N2 was significantly higher than that of Fas staining (P0.05). The integral optical density of the positive expression of TUNEL in the hippocampus of N3ON4 was 96.43 卤20.48 and 156.56 卤22.37respectively, and there was no significant difference between the two groups (P0.05). The positive expression of TUNEL in N2 was significantly higher than that in TUNEL (P0.05). Transmission electron microscope (TEM) was used to observe the chromatin accumulation, condensation and formation of crescents constricting along the nuclear membrane in the hippocampal neurons of the experimental group, and the mitochondria in the cytoplasm were enlarged, vacuolated, and the cytoskeleton was disordered. Cell membrane microvilli, cell processes and intercellular junctions disappeared, apoptotic bodies appeared, synaptic spaces were blurred, chromatin aggregation and synaptic vesicles were not clear. Synaptic vesicles decreased, postsynaptic membranes swelled and colloid membranes scattered around small vessels swelled. Conclusion long term noise exposure can induce neuronal apoptosis and increase the expression of Fas protein in hippocampal area of rats. The expression of apoptosis-related protein Fas may be involved in the regulation of apoptosis.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R363
本文编号：2358281
[Abstract]:Objective to study the effects of airport noise exposure on neuronal apoptosis and Fas protein expression in rat hippocampal area. It provides a theoretical basis for the study of the central nervous system in rats exposed to noise pollution. Methods Sixteen rats were used as experimental models after noise exposure in the laboratory of environmental physics of Zhejiang University. The rats were divided into four groups: the control group was N1N _ 2 for 60 days and 90 days for normal feeding, and the experimental group was N3N _ 4 for 60 days of simulated noise exposure at Xiaoshan airport and 30 days of normal feeding for 30 days after detachment. After heart perfusion, brain sections were made, Fas immunohistochemical staining and in situ apoptosis assay (TUNEL) were performed. The integral optical density of positive cells was analyzed by Image-Pro Plus6.0 software. In addition, the ultrastructure of hippocampal neurons in rats was observed under transmission electron microscope. Results the integral optical density of Fas positive expression was 2.67 卤1.67 脳 10 ~ 3 and 3.03 卤2.18 脳 10 ~ 3 in N _ 1N _ 2 group, respectively. There was no significant difference between them (P0.05). The integral optical density of the positive expression of TUNEL in the hippocampus of N _ 1N _ 2 was 44.10 卤1.67 and 51.75 卤10.90, respectively, and there was no significant difference between the two groups (P0.05). The integral optical density of positive expression of Fas in hippocampus of N4 group was 34.29 卤12.60 脳 10 ~ 3 and 20.54 卤5.69 脳 10 ~ 3, respectively. There was no significant difference between the two groups (P0.05). The positive expression of N2 was significantly higher than that of Fas staining (P0.05). The integral optical density of the positive expression of TUNEL in the hippocampus of N3ON4 was 96.43 卤20.48 and 156.56 卤22.37respectively, and there was no significant difference between the two groups (P0.05). The positive expression of TUNEL in N2 was significantly higher than that in TUNEL (P0.05). Transmission electron microscope (TEM) was used to observe the chromatin accumulation, condensation and formation of crescents constricting along the nuclear membrane in the hippocampal neurons of the experimental group, and the mitochondria in the cytoplasm were enlarged, vacuolated, and the cytoskeleton was disordered. Cell membrane microvilli, cell processes and intercellular junctions disappeared, apoptotic bodies appeared, synaptic spaces were blurred, chromatin aggregation and synaptic vesicles were not clear. Synaptic vesicles decreased, postsynaptic membranes swelled and colloid membranes scattered around small vessels swelled. Conclusion long term noise exposure can induce neuronal apoptosis and increase the expression of Fas protein in hippocampal area of rats. The expression of apoptosis-related protein Fas may be involved in the regulation of apoptosis.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R363
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