酒精摄入导致雌鼠及孕鼠子代血清泌乳素升高及其机制的探讨
发布时间：2018-11-26 19:41
【摘要】：第一部分酒精对雌鼠血清泌乳素水平及垂体湿重的影响 目的探讨酒精对雌鼠血清泌乳素(PRL)水平、垂体湿重的影响,了解摄取酒精的浓度和年龄与血清PRL水平改变及垂体增生的相关性,了解饮酒与高泌乳素血症(HP)的关系。 方法1.40只SD同龄成年雌鼠随机分为4组,每组分别予10%,20%,30%酒精灌胃4周和8周各5只,对照组予等容积和时间蒸馏水灌胃；2.30只4周、6周、8周龄的SD雌鼠分别予以20%酒精灌胃4周,各自对照组均予等容积蒸馏水灌胃；3.各组大鼠处死后均用放射免疫分析法(RIA)检测血清PRL水平,称垂体湿重,分析摄取酒精的浓度和年龄与血清PRL水平、垂体湿重的相关性。 结果1.灌胃4周时,30%酒精组大鼠血清PRL水平和垂体湿重明显高于其它三组；灌胃8周时,10%和20%酒精组明显高于同期30%酒精组和对照组。组内比较,灌胃8周时,10%及20%酒精组大鼠PRL水平和垂体湿重均显著高于灌胃4周时,30%酒精组大鼠PRL水平和垂体湿重显著低于4周时,以上各差异均有统计学意义,P0.05。2.同浓度酒精灌胃4周后,各年龄组与相应的对照组比较,4周龄大鼠血清PRL水平和垂体湿重显著升高；6周龄和8周龄大鼠血清PRL水平、垂体湿重差异均无统计学意义,P0.05。 结论1.酒精摄入可引起雌鼠血清PRL水平发生改变,且有剂量依赖性和累积效应：高浓度酒精可在较短时间内使血清PRL水平升高,长期低浓度酒精摄取可引起PRL水平显著性增高。2.长期大剂量酒精摄入可导致雌性大鼠垂体产生PRL的功能减退。3.较之成年期,幼年期雌性大鼠摄入酒精更易导致血清PRL水平升高。 第二部分摄入酒精对孕鼠子代血清泌乳素及垂体湿重的影响 目的探讨大鼠妊娠期大量摄入酒精对子代血清泌乳素水平、垂体湿重的影响。 方法6只孕鼠随机分为2组,酒精组于怀孕第1天(P1)起给予20%酒精(2g/kg·d)灌胃至分娩前。对照组同期给予等量蒸馏水。子鼠喂养至4周后每组随机抽取一半处死,余下的继续喂养至8周处死。各组大鼠处死后均用放免法检测血清泌乳素水平,并称垂体湿重。 结果1.4周龄子鼠：酒精组的垂体湿重明显高于对照组(P0.05),而两组间PRL水平差异无统计学意义(P0.05)；2.8周龄子鼠：酒精组的PRL水平和垂体湿重均较对照组显著升高(P0.05)。 结论大鼠妊娠期大量摄入酒精可使子代血清泌乳素水平升高、垂体湿重增加。酒精可能通过刺激垂体增生,使得血泌乳素水平升高。 第三部分酒精摄入导致雌鼠血清泌乳素升高机制的探讨 目的：探讨酒精导致雌鼠血清泌乳素升高的机制。 方法：40只大鼠随机分为2组,实验组给予20%酒精2m1灌胃,每日2次共8周,对照组给予等体积蒸馏水灌胃。检测血清泌乳素水平、垂体湿重及免疫组化观察垂体腺细胞增殖情况,库伦阵列电化学高效液相色谱法检测下丘脑匀浆去甲肾上腺素、多巴胺、5-羟色胺、r-氨基丁酸、谷氨酸水平并进行比较。 结果：1.实验组血清泌乳素水平、垂体湿重均显著高于对照组,P0.05。2.实验组下丘脑匀浆多巴胺、5-羟色胺、r-氨基丁酸显著低于对照组,谷氨酸浓度显著高于对照组, P0.05。去甲肾上腺素浓度与对照组比较无显著性差异,P0.05。3.实验组垂体细胞Ki-67的表达显著高于对照组,P0.05。 结论：酒精可以导致泌乳素升高,其机制可能为：1.垂体催乳细胞增殖；2.酒精刺激引起下丘脑泌乳素释放抑制因子(PIF)如多巴胺、5-羟色胺、r-氨基丁酸等分泌减少,而泌乳素释放因子(PRF)如谷氨酸分泌增多。
[Abstract]:The effect of first part of alcohol on serum prolactin level and pituitary-wet weight of female rats Objective To study the effect of alcohol on serum prolactin (PRL) level and pituitary wet weight in female rats, and to understand the concentration and age of intake alcohol and the changes of serum PRL level and the phase of pituitary hyperplasia. To understand the effect of drinking and hyperprolactinemia (HP) The adult female rats of the same age were randomly divided into 4 groups, each group was given 10%, 20%, 30% alcohol for 4 weeks and 8 weeks, and the control group was given equal volume and time distilled water. The control group was given equal volume distillation for 4 weeks. The serum PRL levels were detected by radioimmunoassay (RIA), and the concentration and age of the intake alcohol and the serum PRL level and the pituitary humidity were analyzed by radioimmunoassay (RIA). heavy correlation Results 1. The serum PRL level and the weight of the pituitary in the 30% alcohol group were significantly higher than those of the other three groups at 4 weeks. The 10% and 20% alcohol groups were significantly higher than that of the other three groups at 8 weeks. The levels of PRL in 10% and 20% alcohol group were significantly higher than that in the control group. The levels of PRL and the weight of the pituitary in the 30% alcohol group were significantly lower than that in the 4-week group. The serum PRL level and the pituitary-wet weight of the 4-week-old rats were significantly higher than those of the corresponding control group, and the serum PRL level and the pituitary-wet weight of the 6-week-old and 8-week-old rats were not statistically significant. Significance, P Conclusion 1. Alcohol intake can cause a change in serum PRL level in female rats with dose-dependent and cumulative effects: high-concentration alcohol can raise serum PRL level in a short time, and low-concentration alcohol intake can cause P A significant increase in the level of RL. 2. Long-term high-dose alcohol intake could lead to the pituitary production in female rats The function of the raw PRL is reduced. 3. Compared to the adult, the young female rats are more likely to take alcohol resulting in an elevated serum PRL level. The second part of the intake of alcohol to the pregnant rat Objective To study the effect of prolactinine and the wet weight of pituitary gland in the offspring of rats. Methods Six pregnant rats were randomly divided into two groups, and the alcohol group was given in the first day of pregnancy (P1). 20% alcohol (2g/ kg 路 d The same amount of distilled water was administered to the control group in the same period. The machine was killed by half, and the rest was continued for 8 weeks. All the rats were sacrificed. The serum prolactin level was detected by radioimmunoassay, and the pituitary-wet weight was weighed. The results were as follows: the weight of the pituitary in the alcohol group was significantly higher than that in the control group (P0.05), while the difference of the levels of PRL in the two groups was not significant (P0.05); and the PRL level in the 2. 8-week-old mice: the PRL of the alcohol group. The level and the wet weight of the pituitary gland were significantly higher than that in the control group (P0.05). A large amount of alcohol may increase the serum prolactin level in the offspring and the pituitary wet weight Increased. Alcohol may increase the level of prolactin by stimulating the pituitary gland. Increased. The third part of the alcohol intake resulted in the lactation of female rats Objective: To study the mechanism of the increase of serum prolactin in female rats by alcohol. Methods: 40 rats were randomly divided into 2 groups. The serum prolactin level, pituitary wet weight and immunohistochemistry were used to observe the proliferation of the pituitary gland cells, and the coulombic array electrochemical high performance liquid chromatography was used to detect the hypothalamus. Syrup of norepinephrine adenosine, dopamine, 5-hydroxytryptamine, r-aminobutyric acid, glutamate level and comparison. Results: 1. The serum prolactin level and the weight of the pituitary in the experimental group were significantly higher than that in the control group (P <0.05). The concentration of 5-hydroxytryptamine, r-aminobutyric acid was significantly lower than that of the control group, and the concentration of glutamic acid was significantly higher than that of the control group. The concentration of norepinephrine in the control group was no significant difference with the control group. The expression of Ki-67 in the pituitary cells of the experimental group It was significantly higher than that in the control group..................................................................................................................................................................................................
【学位授予单位】：中南大学
【学位级别】：博士
【学位授予年份】：2010
【分类号】：R363
本文编号：2359444
[Abstract]:The effect of first part of alcohol on serum prolactin level and pituitary-wet weight of female rats Objective To study the effect of alcohol on serum prolactin (PRL) level and pituitary wet weight in female rats, and to understand the concentration and age of intake alcohol and the changes of serum PRL level and the phase of pituitary hyperplasia. To understand the effect of drinking and hyperprolactinemia (HP) The adult female rats of the same age were randomly divided into 4 groups, each group was given 10%, 20%, 30% alcohol for 4 weeks and 8 weeks, and the control group was given equal volume and time distilled water. The control group was given equal volume distillation for 4 weeks. The serum PRL levels were detected by radioimmunoassay (RIA), and the concentration and age of the intake alcohol and the serum PRL level and the pituitary humidity were analyzed by radioimmunoassay (RIA). heavy correlation Results 1. The serum PRL level and the weight of the pituitary in the 30% alcohol group were significantly higher than those of the other three groups at 4 weeks. The 10% and 20% alcohol groups were significantly higher than that of the other three groups at 8 weeks. The levels of PRL in 10% and 20% alcohol group were significantly higher than that in the control group. The levels of PRL and the weight of the pituitary in the 30% alcohol group were significantly lower than that in the 4-week group. The serum PRL level and the pituitary-wet weight of the 4-week-old rats were significantly higher than those of the corresponding control group, and the serum PRL level and the pituitary-wet weight of the 6-week-old and 8-week-old rats were not statistically significant. Significance, P Conclusion 1. Alcohol intake can cause a change in serum PRL level in female rats with dose-dependent and cumulative effects: high-concentration alcohol can raise serum PRL level in a short time, and low-concentration alcohol intake can cause P A significant increase in the level of RL. 2. Long-term high-dose alcohol intake could lead to the pituitary production in female rats The function of the raw PRL is reduced. 3. Compared to the adult, the young female rats are more likely to take alcohol resulting in an elevated serum PRL level. The second part of the intake of alcohol to the pregnant rat Objective To study the effect of prolactinine and the wet weight of pituitary gland in the offspring of rats. Methods Six pregnant rats were randomly divided into two groups, and the alcohol group was given in the first day of pregnancy (P1). 20% alcohol (2g/ kg 路 d The same amount of distilled water was administered to the control group in the same period. The machine was killed by half, and the rest was continued for 8 weeks. All the rats were sacrificed. The serum prolactin level was detected by radioimmunoassay, and the pituitary-wet weight was weighed. The results were as follows: the weight of the pituitary in the alcohol group was significantly higher than that in the control group (P0.05), while the difference of the levels of PRL in the two groups was not significant (P0.05); and the PRL level in the 2. 8-week-old mice: the PRL of the alcohol group. The level and the wet weight of the pituitary gland were significantly higher than that in the control group (P0.05). A large amount of alcohol may increase the serum prolactin level in the offspring and the pituitary wet weight Increased. Alcohol may increase the level of prolactin by stimulating the pituitary gland. Increased. The third part of the alcohol intake resulted in the lactation of female rats Objective: To study the mechanism of the increase of serum prolactin in female rats by alcohol. Methods: 40 rats were randomly divided into 2 groups. The serum prolactin level, pituitary wet weight and immunohistochemistry were used to observe the proliferation of the pituitary gland cells, and the coulombic array electrochemical high performance liquid chromatography was used to detect the hypothalamus. Syrup of norepinephrine adenosine, dopamine, 5-hydroxytryptamine, r-aminobutyric acid, glutamate level and comparison. Results: 1. The serum prolactin level and the weight of the pituitary in the experimental group were significantly higher than that in the control group (P <0.05). The concentration of 5-hydroxytryptamine, r-aminobutyric acid was significantly lower than that of the control group, and the concentration of glutamic acid was significantly higher than that of the control group. The concentration of norepinephrine in the control group was no significant difference with the control group. The expression of Ki-67 in the pituitary cells of the experimental group It was significantly higher than that in the control group..................................................................................................................................................................................................
【学位授予单位】：中南大学
【学位级别】：博士
【学位授予年份】：2010
【分类号】：R363
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