嗜酸性粒细胞抗肿瘤作用及其机制的初步研究

发布时间：2018-12-06 14:08

【摘要】： 目的: 嗜酸性粒细胞(eosinophil,EOS)由骨髓造血干细胞增殖分化而来,嗜酸性粒细胞增高除见寄生虫感染过敏及皮肤病外,也见于多种肿瘤及血液病。本实验初步研究嗜酸性粒细胞抗肿瘤作用,探讨其抗肿瘤及其免疫调节机制。方法: 将人白血病细胞K562和胃癌细胞SGC-7901与从致敏小鼠血液和腹水中分离出来不同浓度的嗜酸性粒细胞共同培育12h-48小时,应用生长曲线法、MTT比色法来检测EOS对白血病细胞K562和胃癌细胞SGC-7901细胞株增殖的影响。同时在小鼠右腋皮下接种肉瘤细胞(S_(180)),建立荷S_(180)肉瘤小鼠模型,24小时后随机分为4组:空白对照组、阴性对照组、阳性对照组、EOS治疗组。观察荷瘤小鼠肿瘤组织生长情况,处死动物分离肿瘤组织,计算抑瘤率;HE染色观察瘤组织细胞形态;MTT法检测荷瘤小鼠淋巴细胞增殖能力;放射免疫法测定小鼠血清中IL-4、TNF-a的含量。结果: 1、血中EOS与胃癌SGC-7901细胞共培养,在48h内没有表现出明显抑制作用(P＞0.05)。血液、腹腔液EOS与白血病K562细胞共培养,36h后表现出不同程度的抑制作用(P＜0.05),血液和腹腔液EOS抑制率分别可达19%和22%。 2、EOS对荷瘤小鼠的肿瘤组织的生长有抑制作用,抑瘤率为16%。 3、在ConA刺激下,EOS治疗组小鼠T淋巴细胞增殖反应能力明显增强(P＜0.05),与阴性对照组比较,EOS治疗组小鼠血清IL-4(0.69±0.32ng/ml)、TNF-a(1.36±0.36ng/ml)水平显著升高,有统计学意义(P＜0.05)。结论 1、血液和腹腔液EOS均对白血病K562细胞株的增殖有抑制作用,尤以血液EOS抑制K562细胞株的增殖最强。 2、EOS对荷S_(180)肉瘤小鼠的肿瘤组织生长有抑制作用,且促进荷瘤鼠T淋巴细胞增殖及细胞因子IL-4、TNF-a的产生,提示EOS对荷瘤小鼠的免疫功能有正调节活性。
[Abstract]:Objective: eosinophilic granulocyte (eosinophil,EOS) is derived from the proliferation and differentiation of bone marrow hematopoietic stem cells. In this study, we studied the anti-tumor effect of eosinophil and its anti-tumor mechanism. Methods: human leukemia cell line K562 and gastric cancer cell SGC-7901 were cultured with different concentrations of eosinophils from sensitized mouse blood and ascites for 12h-48 hours. MTT colorimetric assay was used to detect the effect of EOS on the proliferation of leukemia cell line K562 and gastric cancer cell line SGC-7901. At the same time, mice were inoculated subcutaneously with sarcoma cells (S180),) in the right axilla of mice. After 24 hours, the mice were randomly divided into four groups: blank control group, negative control group, positive control group and EOS treatment group. Tumor tissue growth was observed in tumor-bearing mice, tumor tissue was isolated from animals and tumor inhibition rate was calculated. HE staining was used to observe the morphology of tumor tissue, and lymphocyte proliferation ability was detected by MTT method. The content of IL-4,TNF-a in serum of mice was determined by radioimmunoassay. Results: 1. There was no obvious inhibition of EOS in blood and SGC-7901 cells in gastric cancer within 48 hours (P > 0. 05). Blood, peritoneal fluid EOS and leukemic K562 cells were co-cultured. After 36 hours, the inhibition rates of EOS in blood and peritoneal fluid were 19% and 22% respectively (P < 0. 05). (2) EOS could inhibit the growth of tumor tissue in tumor-bearing mice, and the inhibition rate was 16%. 3. Under the stimulation of ConA, the proliferative ability of T lymphocytes in the EOS treatment group was significantly enhanced (P < 0. 05). Compared with the negative control group, the serum IL-4 in the EOS treatment group was (0. 69 卤0.32ng/ml). The level of TNF-a (1. 36 卤0.36ng/ml) was significantly increased (P < 0. 05). Conclusion 1. Blood and peritoneal fluid EOS can inhibit the proliferation of leukemia K562 cell line, especially blood EOS can inhibit the proliferation of K562 cell line. 2EOS could inhibit the growth of tumor tissue and promote the proliferation of T lymphocytes and the production of cytokine IL-4,TNF-a in mice bearing S180 sarcoma, suggesting that EOS has a positive regulatory activity on the immune function of mice bearing S180.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392

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