急性心理应激对胃肠功能影响的初步研究

发布时间：2018-12-11 18:18

【摘要】：目的：研究急性心理应激对胃肠粘膜形态和屏障功能以及结肠运动的影响。方法： 55只雄性wistar大鼠经过3小时限动束缚后随机分入4个组：A组(3小时即时处死组)14只,B组(束缚后休息3小时处死组)14只,C组(束缚后休息9小时处死组)14只,D组(束缚后休息21小时处死组)13只；另外13只大鼠禁食禁水3小时作为对照；造模其间计数大鼠排便数。在各自时间点解剖大鼠采集标本。肉眼评估胃粘膜、回肠粘膜、结肠粘膜形态；光镜下对胃、回肠、结肠粘膜病理形态进行观察；免疫组化法分析回肠、结肠粘膜紧密连接Occludin蛋白的表达；TUNEL法检测回肠上皮细胞凋亡；检测血浆二胺氧化酶(DAO)活性和D-乳酸(D-lac)浓度。结果：束缚成功造成大鼠急性心理应激；急性心理应激导致大鼠胃粘膜广泛糜烂和散在出血；急性心理应激组紧密连接蛋白Occludin蛋白染色变淡,平均光密度略有降低,但与对照组差异不显著；急性心理应激组回肠粘膜上皮细胞凋亡指数明显增高。和对照组比较,急性心理应激各组血浆DAO活性和D-lac浓度未见明显增高；急性心理应激导致大鼠结肠运动增强。结论： 1本束缚方法制造心理应激模型是成功的。 2本心理应激模型可导致大鼠胃肠粘膜从形态学损害到屏障功能的损害。 3本研究中大鼠肠粘膜屏障功能损害可能以细胞凋亡增加为主要途径,细胞间隙紧密连接破坏为次要途径。 4急性心理应激中大鼠结肠运动增强。
[Abstract]:Aim: to study the effects of acute psychological stress on gastrointestinal mucosal morphology, barrier function and colon motility. Methods: 55 male wistar rats were randomly divided into 4 groups after 3 hours of restraint: group A (group A: n = 14), group B (group B: 3 hours after 3 hours rest), group B (n = 14). There were 14 rats in group C and 13 rats in group D (death group with 21 hours rest after restraint). The other 13 rats were fasting for 3 hours as control, and the defecation number of rats was counted. The rats were dissected at different time points. The morphology of gastric, ileal and colonic mucosa was evaluated by naked eye, the pathological morphology of gastric, ileal and colonic mucosa was observed under light microscope, the expression of Occludin protein in ileum and colonic mucosa was analyzed by immunohistochemical method. TUNEL assay was used to detect the apoptosis of ileal epithelial cells, the plasma diamine oxidase (DAO) activity and the concentration of D-lactic acid (D-lac) were detected. Results: acute psychological stress resulted in extensive erosion and scattered bleeding of gastric mucosa in rats. In acute psychological stress group, the staining of tight junction protein Occludin protein became lighter, the average optical density decreased slightly, but there was no significant difference between the acute psychological stress group and the control group, and the apoptosis index of ileal mucosal epithelial cells in the acute psychological stress group was significantly higher than that in the control group. Compared with the control group, the plasma DAO activity and D-lac concentration in the acute psychological stress groups were not significantly increased, but the colonic motility was increased in the rats under acute psychological stress. Conclusion: 1 the model of psychological stress made by this restraint method is successful. 2 the psychological stress model can cause the damage of gastrointestinal mucosa from morphological damage to barrier function in rats. 3 in this study, the main pathway of intestinal mucosal barrier dysfunction in rats was the increase of apoptosis, and the destruction of tight junction between the cells was the secondary pathway. 4 the colonic motility of rats was enhanced during acute psychological stress.
【学位授予单位】：北京协和医学院
【学位级别】：博士
【学位授予年份】：2009
【分类号】：R363

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