雌马酚对血管内皮细胞凋亡和增殖影响
发布时间：2018-12-13 08:25
【摘要】：目的探讨大豆异黄酮(SI)代谢物雌马酚对血管内皮细胞凋亡和增殖的影响。方法体外培养血管内皮细胞(ECV-304),采用不同浓度雌马酚(0、6.25、12.50、25.00、50.00和100.00μmol/L)处理24 h,收集细胞,采用流式细胞术分析细胞内活性氧(ROS)、细胞凋亡和细胞周期;采用免疫组织化学技术分析凋亡相关蛋白Bax、Bcl-2和caspase-3表达;采用噻唑蓝(MTT)法分析雌马酚对ECV-304细胞增殖影响。结果与对照组比较,6.25μmol/L雌马酚组ECV-304细胞内ROS含量下降,100.00μmol/L雌马酚组ECV-304细胞内ROS含量升高;与对照组比较,25.00、50.00、100.00μmol/L雌马酚组ECV-304细胞早、晚期凋亡率明显升高,并可导致细胞G1期阻滞(P0.05);MTT实验结果显示,0、6.25、12.50、25.00、50.00、100.00μmol/L雌马酚组ECV-304细胞增殖抑制率分别为-(8.23±2.21)%、-(3.29±1.07)%、(6.75±1.73)%、(25.46±4.82)%和(36.93±4.66)%,低剂量雌马酚对细胞生长具有促进作用,高剂量雌马酚对细胞生长具有抑制作用;与对照组比较,25.00μmol/L雌马酚组作用24 h后,细胞内Bax和caspase-3表达增加,而Bcl-2表达减少。结论低浓度雌马酚可降低细胞内ROS含量,促进ECV-304细胞增殖;高浓度雌马酚可导致细胞凋亡、细胞周期阻滞,抑制细胞增殖。
[Abstract]:Objective to investigate the effect of estradiol, a metabolite of soybean isoflavone (SI), on apoptosis and proliferation of vascular endothelial cells. Methods Vascular endothelial cells (ECV-304) were cultured in vitro and treated with different concentrations of estradiol (0.6.25A12.5012.50,25.0050.00 and 100.00 渭 mol/L) for 24 hours. The cells were collected and analyzed by flow cytometry for intracellular reactive oxygen species (ROS),). Apoptosis and cell cycle; The expression of apoptosis-related protein Bax,Bcl-2 and caspase-3 was analyzed by immunohistochemistry and the effect of estradiol on the proliferation of ECV-304 cells was analyzed by thiazolyl (MTT) assay. Results compared with the control group, the content of ROS in ECV-304 cells in 6.25 渭 mol/L female equisol group decreased, and the ROS content in ECV-304 cells increased in 100.00 渭 mol/L estralophen group. Compared with the control group, the ECV-304 cells in the 25.00 渭 mol/L 50.00100.00 渭 mol/L group were early, the late apoptosis rate was significantly increased, and the G1 phase arrest was induced (P0.05). The results of MTT test showed that the inhibition rate of ECV-304 cell proliferation was-(8.23 卤2.21)%, (3.29 卤1.07)%, (6.75 卤1.73)% and (6.75 卤1.73)%, respectively. (25.46 卤4.82)% and (36.93 卤4.66)% respectively. Low dose of estradiol promoted cell growth, and high dose of female equisol inhibited cell growth. Compared with the control group, the expression of Bax and caspase-3 increased and the expression of Bcl-2 decreased in 25.00 渭 mol/L female equallol group for 24 h. Conclusion low concentration of estradiol can reduce the content of ROS and promote the proliferation of ECV-304 cells, and high concentration of estradiol can induce cell apoptosis, arrest cell cycle and inhibit cell proliferation.
【作者单位】： 吉林医药学院心理学教研室;吉林医药学院检验学院;吉林医药学院药学院;
【基金】：吉林省教育厅“十二五”科学技术研究项目(2012335) 国家自然科学基金(82102953) 吉林省科技厅中青年科技创新领军人才及团队项目(20130521018JH) 吉林省科技厅科技攻关重大项目(20140203012YY)
【分类号】：R3411
本文编号：2376237
[Abstract]:Objective to investigate the effect of estradiol, a metabolite of soybean isoflavone (SI), on apoptosis and proliferation of vascular endothelial cells. Methods Vascular endothelial cells (ECV-304) were cultured in vitro and treated with different concentrations of estradiol (0.6.25A12.5012.50,25.0050.00 and 100.00 渭 mol/L) for 24 hours. The cells were collected and analyzed by flow cytometry for intracellular reactive oxygen species (ROS),). Apoptosis and cell cycle; The expression of apoptosis-related protein Bax,Bcl-2 and caspase-3 was analyzed by immunohistochemistry and the effect of estradiol on the proliferation of ECV-304 cells was analyzed by thiazolyl (MTT) assay. Results compared with the control group, the content of ROS in ECV-304 cells in 6.25 渭 mol/L female equisol group decreased, and the ROS content in ECV-304 cells increased in 100.00 渭 mol/L estralophen group. Compared with the control group, the ECV-304 cells in the 25.00 渭 mol/L 50.00100.00 渭 mol/L group were early, the late apoptosis rate was significantly increased, and the G1 phase arrest was induced (P0.05). The results of MTT test showed that the inhibition rate of ECV-304 cell proliferation was-(8.23 卤2.21)%, (3.29 卤1.07)%, (6.75 卤1.73)% and (6.75 卤1.73)%, respectively. (25.46 卤4.82)% and (36.93 卤4.66)% respectively. Low dose of estradiol promoted cell growth, and high dose of female equisol inhibited cell growth. Compared with the control group, the expression of Bax and caspase-3 increased and the expression of Bcl-2 decreased in 25.00 渭 mol/L female equallol group for 24 h. Conclusion low concentration of estradiol can reduce the content of ROS and promote the proliferation of ECV-304 cells, and high concentration of estradiol can induce cell apoptosis, arrest cell cycle and inhibit cell proliferation.
【作者单位】： 吉林医药学院心理学教研室;吉林医药学院检验学院;吉林医药学院药学院;
【基金】：吉林省教育厅“十二五”科学技术研究项目(2012335) 国家自然科学基金(82102953) 吉林省科技厅中青年科技创新领军人才及团队项目(20130521018JH) 吉林省科技厅科技攻关重大项目(20140203012YY)
【分类号】：R3411
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