大鼠蛛网膜下腔出血致脑血管痉挛模型的建立

发布时间：2018-12-15 00:27

【摘要】：目的:应用改良的枕大池二次注血法建立大鼠蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后脑血管痉挛(cerebral vasospasm,CVS)模型。方法:成年雄性SD大鼠117只随机分为假手术组(n=52)和SAH(n=65)模型组,2组又随机分为1、3、5、10 d和14 d组。SAH组经改良的枕大池二次注血法建立SAH模型。假手术组用等量灭菌生理盐水代替非抗凝自体血注入枕大池。观察各组动物自发活动、脑含水量、脑组织学及基底动脉组织学改变。结果:与假手术组相比,SAH各组大鼠自发活动评分在手术后第3天明显降低(P=0.001),脑含水量在SAH后1 d明显增加(P=0.000)。光镜下,SAH 1、3、5 d组基底动脉管径(P=0.001)、管腔面积明显减小(P=0.002),管壁厚度明显增大(P=0.000),差异均有统计学意义。结论:应用改良的枕大池二次注血法可以成功建立大鼠SAH后CVS模型。
[Abstract]:Aim: to establish a model of cerebral vasospasm (cerebral vasospasm,CVS) after subarachnoid hemorrhage (subarachnoid hemorrhage,SAH) in rats by using the modified blood injection method of cistern magnum. Methods: 117 adult male SD rats were randomly divided into two groups: sham operation group (n = 52) and SAH model group (n = 65), and two groups were randomly divided into two groups: group 1, 3, 5, 5, 10 and 14 days. SAH model was established in SAH group by modified blood injection of cistern fortuitalis. In sham operation group, the same amount of sterilized saline was injected into the cistern instead of non-anticoagulant autologous blood. The spontaneous activity, brain water content, brain histology and histological changes of basilar artery were observed. Results: compared with the sham operation group, the spontaneous activity score of the rats in the SAH group was significantly decreased on the 3rd day after operation (P0. 001), and the brain water content was significantly increased 1 day after SAH (P0. 000). Under light microscope, the diameter of basilar artery (P0. 001), lumen area (P0. 002) and wall thickness (P0. 000) of SAH group were significantly decreased (P0. 001). Conclusion: the model of CVS after SAH can be successfully established by using the modified method of second injection of blood into the cistern of occipital cistern.
【作者单位】：重庆医科大学神经科学研究中心/生理学教研室;泸州医学院生理教研室;
【基金】：国家自然科学基金青年基金资助项目(编号:81100453)
【分类号】：R743.35;R-332

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