康妇炎胶囊对兔盆腔炎性疾病后遗症模型粘连相关指标影响的实验研究

发布时间：2018-12-16 10:00

【摘要】：目的:通过观察康妇炎胶囊对盆腔炎性疾病(PID)后遗症模型兔子宫、输卵管组织粘连相关指标细胞间粘附分子-1(ICAM-1)、肿瘤坏死因子-α(TNF-a)、血管内皮生长因子(VEGF)表达的影响,从抗粘连方面探讨康妇炎胶囊治疗盆腔炎性疾病后遗症的机制。方法:将5~6个月龄新西兰雌兔分为:正常组、模型组、桂枝茯苓组、康妇炎组,除正常组外,其余3组均通过逆行宫腔插管灌注细菌法建立盆腔炎模型,造模成功后,模型组、桂枝茯苓组、康妇炎组分别灌服蒸馏水、桂枝茯苓胶囊、康妇炎胶囊3周,正常组灌服蒸馏水。肉眼观察各组兔子宫及输卵管情况;HE染色观察各组兔子宫、输卵管组织学变化;免疫组化及免疫印迹法检测各组兔子宫、输卵管组织ICAM-1、TNF-a、VEGF表达的变化。结果:肉眼观察模型组兔腹膜明显增厚,盆腔积液,子宫充血,质脆,弹性差,输卵管管壁粗糙、增厚,与周围组织粘连,或外观略呈串珠样;康妇炎组兔腹膜增厚不明显,无盆腔积液,子宫无明显充血,输卵管与周围组织无明显粘连;康妇炎组兔子宫、输卵管组织ICAM-1、TNF-a、VEGF表达较模型组明显降低(P0.05或P0.01)。结论:康妇炎胶囊治疗PID后遗症可能与其降调子宫、输卵管组织粘连相关指标ICAM-1、TNF-α、VEGF的表达有关。
[Abstract]:Objective: to observe the effects of Kangfuyan capsule on rabbit uterus and fallopian tube tissue adhesion related index (ICAM-1), tumor necrosis factor- 伪 (TNF-a), and to observe the effect of Kangfuyan capsule on (PID) sequelae of pelvic inflammatory disease. The effect of vascular endothelial growth factor (VEGF) expression on the mechanism of Kangfuyan capsule in treating the sequelae of pelvic inflammatory disease was discussed from the aspect of anti-adhesion. Methods: five to six months old New Zealand female rabbits were divided into normal group, model group, Guizhi Fuling group and Kangfuyan group. Except the normal group, the other three groups were established pelvic inflammatory model by retrograde intrauterine intubation with bacteria. Guizhi Fuling group and Kangfuyan group were given distilled water, Guizhi Fuling capsule and Kangfuyan capsule for 3 weeks, respectively. The rabbit uterus and fallopian tube were observed with naked eye; the histological changes of rabbit uterus and fallopian tube were observed by HE staining; the changes of ICAM-1,TNF-a,VEGF expression in rabbit uterus and fallopian tube were detected by immunohistochemistry and Western blotting. Results: in the model group, peritoneal thickening, pelvic effusion, hyperemia, brittleness, poor elasticity, rough and thickened tubal wall, adhesion with surrounding tissues, or appearance were observed. In Kangfuyan group, the peritoneal thickening was not obvious, there was no pelvic effusion, no hyperemia in uterus, and no obvious adhesion between fallopian tube and surrounding tissues. The expression of ICAM-1,TNF-a,VEGF in rabbit uterus and fallopian tube in Kangfuyan group was significantly lower than that in model group (P0.05 or P0.01). Conclusion: the treatment of PID sequelae with Kangfuyan capsule may be related to the expression of ICAM-1,TNF- 伪 and VEGF in uterus and fallopian tube tissue adhesion.
【作者单位】：陕西中医药大学附属医院妇科;
【基金】：陕西省教育厅项目(14JK1198)
【分类号】：R285.5;R-332

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