日本血吸虫SjC23-Hsp70DNA疫苗与IL-12对水牛保护性作用的研究
发布时间：2018-12-18 00:35
【摘要】： 目的:研究日本血吸虫中国大陆株23 kDa膜蛋白-热休克蛋白(SjC23-Hsp70)DNA疫苗联合佐剂白细胞介素-12(IL-12)质粒DNA对水牛的免疫保护作用。 方法:将购自血吸虫病非流行区8-10月龄健康水牛45头随机分为A组(SjC23-Hsp70+IL-12,300μg)、B组(SjC23+IL-12,300μg)和C组(pVAX+IL-12,300μg)等3组,每组15头。每头牛经肩部肌注免疫3次,每次间隔为28天。末次免疫后28天,每头牛感染日本血吸虫尾蚴1000条。解剖前2天及当天分别收集粪便1次,用定量法检测虫卵和毛蚴数。攻击感染后56天解剖所有水牛,经胸主动脉灌冲法收集成虫,计数成虫数,检测每克肝组织虫卵数。试验采用双盲法,所用疫苗及制剂均在实验结束后解码。 结果:A、B组与C组相比,分别获得45.70%和26.61%的减雌率,44.51%和25.84%的减虫率,41.10%和31.63%的减粪卵率,48.11%和38.07%的减毛蚴率及43.39%和31.95%的减肝卵率。A组的5个率均比B组高。 结论:用SjC23-Hsp70+DNA疫苗和IL-12联合免疫水牛可获得明显的免疫保护作用。
[Abstract]:Aim: to study the protective effect of 23 kDa membrane protein-heat shock protein (SjC23-Hsp70) DNA vaccine combined with adjuvant interleukin-12 (IL-12) plasmid DNA on buffalo. Methods: 45 healthy buffalo were randomly divided into three groups: group A (SjC23-Hsp70 IL-12300 渭 g), B (SjC23 IL-12300 渭 g) and group C (pVAX IL-12300 渭 g). Each cow was immunized with shoulder intramuscular injection for 3 times, with 28 days interval. 28 days after the last immunization, each cow was infected with 1000 cercariae of Schistosoma japonicum. The feces were collected 2 days before dissection and once on the same day, and the eggs and the number of cercariae were detected by quantitative method. All buffaloes were dissected 56 days after attack. Adult worms were collected by thoracic aortic perfusion. The number of adult worms was counted and the number of eggs per gram of liver tissue was detected. The double-blind method was used and the vaccine and preparation were decoded after the experiment. Results: 45.70% and 26.61% female reduction rate, 44.51% and 25.84% worm reduction rate, 41.10% and 31.63% fecal egg reduction rate were obtained in group A B and C, respectively. 48.11% and 38.07% of the cases and 43.39% and 31.95% of the liver oocytes were reduced respectively. The five rates in group A were higher than those in group B. Conclusion: buffalo immunized with SjC23-Hsp70 DNA vaccine and IL-12 can obtain obvious immune protective effect.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392
[Abstract]:Aim: to study the protective effect of 23 kDa membrane protein-heat shock protein (SjC23-Hsp70) DNA vaccine combined with adjuvant interleukin-12 (IL-12) plasmid DNA on buffalo. Methods: 45 healthy buffalo were randomly divided into three groups: group A (SjC23-Hsp70 IL-12300 渭 g), B (SjC23 IL-12300 渭 g) and group C (pVAX IL-12300 渭 g). Each cow was immunized with shoulder intramuscular injection for 3 times, with 28 days interval. 28 days after the last immunization, each cow was infected with 1000 cercariae of Schistosoma japonicum. The feces were collected 2 days before dissection and once on the same day, and the eggs and the number of cercariae were detected by quantitative method. All buffaloes were dissected 56 days after attack. Adult worms were collected by thoracic aortic perfusion. The number of adult worms was counted and the number of eggs per gram of liver tissue was detected. The double-blind method was used and the vaccine and preparation were decoded after the experiment. Results: 45.70% and 26.61% female reduction rate, 44.51% and 25.84% worm reduction rate, 41.10% and 31.63% fecal egg reduction rate were obtained in group A B and C, respectively. 48.11% and 38.07% of the cases and 43.39% and 31.95% of the liver oocytes were reduced respectively. The five rates in group A were higher than those in group B. Conclusion: buffalo immunized with SjC23-Hsp70 DNA vaccine and IL-12 can obtain obvious immune protective effect.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392
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