孕期亚临床甲减大鼠模型的建立

发布时间：2018-12-20 05:54

【摘要】：目的建立可靠的孕期亚临床甲减大鼠动物模型。方法将60只成年雌性Wistar大鼠随机分为三组:1完全甲减组:手术完全切除甲状腺;2亚临床甲减组:手术完全切除甲状腺后,予L-T4微渗泵1.0μg/(100 g·d)浓度持续皮下泵入,自怀孕第15 d剂量改为1.05μg/(100 g·d);3正常对照组:行甲状腺假手术。各组大鼠在基础状态、孕前和怀孕后第10、13、17 d和分娩当天采血测定甲状腺功能。结果各组大鼠在基础状态时,血清TSH和TT4水平差异无统计学意义(P0.05)。完全甲减组大鼠在孕前和整个怀孕过程中及分娩当天血清TSH水平始终明显高于亚临床甲减组和正常对照组(P均0.01),血清TT4水平始终明显低于其他两组(P均0.01)。亚临床甲减组大鼠在孕前和整个怀孕过程中及分娩当天血清TSH水平始终明显高于正常对照组(P均0.05),血清TT4水平始终与正常对照组相当(P均0.05)。结论应用手术完全切除甲状腺,再予微渗泵皮下持续输入合适剂量的L-T4可以成功建立孕期亚临床甲减大鼠模型。
[Abstract]:Objective to establish a reliable animal model of subclinical hypothyroidism during pregnancy. Methods 60 adult female Wistar rats were randomly divided into three groups: 1 complete hypothyroidism group: complete thyroidectomy; 2Subclinical hypothyroidism group: after total thyroidectomy, L-T4 microosmotic pump 1.0 渭 g / (100 g d) was continuously injected subcutaneously, and the dose was changed to 1.05 渭 g / (100 g d);) from the 15th day of pregnancy. 3 normal control group: thyroid sham-operation was performed. The thyroid function was measured at the basal state, at 1317 days before pregnancy and on the 10th day of pregnancy and on the day of parturition. Results there was no significant difference in serum TSH and TT4 levels between the three groups (P0.05). The serum TSH level in the complete hypothyroidism group was significantly higher than that in the subclinical hypothyroidism group and the normal control group before pregnancy and during pregnancy and on the day of delivery (P0.01), and the serum TT4 level was significantly lower than that in the other two groups (P0.01). The serum TSH levels in the subclinical hypothyroidism group were significantly higher than those in the normal control group before pregnancy, during pregnancy and on the day of delivery. The serum TT4 level was always similar to that of the normal control group (P0. 05). Conclusion the model of subclinical hypothyroidism in pregnant rats can be successfully established by surgical resection of thyroid gland and subcutaneous continuous infusion of appropriate dose of L-T4 with microosmotic pump.
【作者单位】：中国医科大学附属第一医院内分泌科;中国医科大学内分泌研究所辽宁省内分泌疾病重点实验室;
【基金】：高等学校博士学科点专项科研基金(项目编号:20092104120003)
【分类号】：R714.256;R-332

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