脑梗死大鼠模型神经功能缺损评分的动态变化分析

发布时间：2019-01-01 08:00

【摘要】：目的:探讨脑梗死大鼠模型的神经功能缺损评分的动态变化。方法:将Zea-longa线拴法复制大脑中动脉缺血(MCAO)大鼠模型,进行提插手法针刺治疗,每12h 1次,共干预6次。以神经行为学、脑血流、梗死率、微循环、光镜等为测量指标。脑梗死大鼠模型于第12、24、36、48、60、72h针刺后进行6次神经行为学评分,借助因子分析方法,结合SPSS 17.0统计分析软件,将神经功能缺损评分结果分别与梗死率、脑血流、微循环、光镜等指标数据进行权重系数确定。结果:脑梗死大鼠的6次神经行为学评分的权重系数分别为:0.173645、0.129477、0.271616、0.125729、0.132866、0.132859。结论:对于神经行为学指标来说,针刺第3次即36h处的权重最大,在针刺第4次以后各个指标的权重变化不是很大。可见,针刺第3次即36h处是行为学指标变化的关键节点,针刺48h以后脑梗死大鼠病情逐步处于稳定状态。
[Abstract]:Objective: to investigate the dynamic changes of neurological deficit score in rats with cerebral infarction. Methods: the rat model of middle cerebral artery ischemia (MCAO) was established by Zea-longa thread ligation, and treated with lifting and meddling acupuncture every 12 hours for 6 times. Neurobehavioral, cerebral blood flow, infarct rate, microcirculation and light microscope were used as the index. The rat model of cerebral infarction was treated with acupuncture for 6 times after acupuncture at 1224 h, 36 minutes, 48 minutes and 6072 hours. With the help of factor analysis method and SPSS 17.0 statistical analysis software, the neurological deficit score was correlated with the infarct rate, cerebral blood flow and microcirculation, respectively. Light mirror and other index data to determine the weight coefficient. Results: the weight coefficients of 6 neurobehavioral scores in rats with cerebral infarction were 0.173645 / 0.129477 / 0.271616 / 0.1257290.13286/ 0.1328599.Results: the weight coefficients of 6 neurobehavioral scores in rats with cerebral infarction were 0.173645 / 0.129477 / 0.271616 / 0.1257290.13286/ 0.132859. Conclusion: for the neurobehavioral indexes, the weight of the third acupuncture is the largest at 36h, but the weight of each index is not changed much after the fourth acupuncture. It can be seen that the third time of acupuncture is the key node of behavioral index change at 36h, and the condition of cerebral infarction rats is stable gradually after 48 hours of acupuncture.
【作者单位】：江西中医药大学附属医院;天津中医药大学第一附属医院;国家中医管理局脑病针刺疗法重点研究室;国家中医药管理局针刺量效关系三级实验室;天津工业大学数理学院;天津市针灸学重点实验室;
【基金】：国家自然科学基金项目(No.81360607) 国家重点基础研究发展计划(973计划)项目(No.2012CB518505,No.2006CB504504)~~
【分类号】：R-332;R245

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