载脂蛋白M在大鼠体内清除及分布的研究

发布时间：2019-01-07 22:29

【摘要】： 目的:载脂蛋白M(apolipoproteinM, ApoM)是新近发现的一种人类HDL载脂蛋白,它通过影响前β-HDL的形成,调节胆固醇的出胞机制,是HDL代谢的重要调节因子,并对人类动脉粥样硬化的病变发展起重要作用。但载脂质蛋白M清除代谢过程尚未完全清楚,我们用放射性同位素~(125)I标记载脂蛋白M,来研究其在大鼠体内的清除及分布情况。方法:用~(125)I标记重组apoM,注入大鼠尾静脉,于不同时间点取血及各个脏器及组织,研究apoM在大鼠体内的清除和分布。结果:~(125)I-apoM的标记率为98 %,大鼠血液中清除过程曲线符合二房室开放型模型,分布相半衰期T1/ 2 (α) = 0. 485 h ,消除相半衰期T1/ 2 (β) = 9.680 h,分数清除速率(fraction clearance rate , FCR) = 11.960 /h。apoM在大鼠组织器官的分布用每克组织放射性表示。胃分布最高,小肠、肝、肺、肾分布次之,脑分布最低,且每克组织放射性随时间增加逐渐降低。将放射性换算成总组织放射性时,小肠最高,胃和肝次之。结论:ApoM在大鼠血液中清除过程曲线符合二房室开放型模型,在血液中分数清除速率FCR=11.960 /h。~(125)I-apoM主要在胃、小肠和肝被结合摄取,小肠、胃和肝可能存在结合及摄取~(125)I-apoM结合位点,胃肠及肝脏可能是参与apoM代谢的重要器官,并可能存在apoM的受体。
[Abstract]:Objective: apolipoprotein M (apolipoproteinM, ApoM) is a newly discovered human HDL apolipoprotein. It regulates the mechanism of cholesterol exocytosis by affecting the formation of pre- 尾-HDL, and is an important regulator of HDL metabolism. It also plays an important role in the development of human atherosclerosis. However, the metabolism of apolipoprotein M was not completely clear. We used radioisotope ~ (125) I to label apolipoprotein M to study its clearance and distribution in rats. Methods: 125i labeled recombinant apoM, was injected into the caudal vein of rats. Blood samples, organs and tissues were collected at different time points to study the clearance and distribution of apoM in rats. Results: the labeling rate of ~ (125) I-apoM was 98%. The blood clearance curve of rats was in accordance with the two-compartment open model, and the distribution half-life T _ 1 / 2 (伪) = 0. For 485h, the half-life of elimination phase T _ 1 / 2 (尾) = 9.680 h, fractional clearance rate (fraction clearance rate, FCR) = 11.960 / h.apoM in rat tissues and organs were expressed by radioactivity per gram of tissue. The distribution of stomach was the highest, the distribution of small intestine, liver, lung and kidney was the second, the distribution of brain was the lowest, and the radioactivity per gram of tissue decreased gradually with the increase of time. When radioactivity was converted to total tissue radioactivity, the small intestine was the highest, the stomach and liver were the second. Conclusion: the clearance process curve of ApoM in rat blood accords with the two-compartment open model. The fractional clearance rate of FCR=11.960 / h.125 I-apoM in the blood is mainly absorbed in the stomach, small intestine and liver, and the small intestine. There may be binding and uptake sites of ~ (125) I-apoM in stomach and liver. Gastrointestinal tract and liver may be important organs involved in the metabolism of apoM, and there may be receptors for apoM.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R341

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