骨髓源神经干细胞对神经胶质瘤趋化性的研究
发布时间：2019-01-19 18:12
【摘要】： 目的探讨骨髓源神经干细胞(NSCs)对神经胶质瘤的定向迁移能力及分布规律。方法从雄性Wistar大鼠股骨和胫骨分离纯化骨髓基质干细胞(BMSCs),加碱性成纤维细胞生长因子(bFGF)和表皮生长因子(EGF)诱导分化为NSCs,并进行免疫组化鉴定。立体定向接种C6胶质瘤细胞于Wistar大鼠脑内基底节区建立鼠脑胶质瘤模型,7d后将用5-溴脱氧尿嘧啶(BrdU)标记的RSCs接种入鼠脑对侧半球,对侧脑室和鼠尾静脉,NSCs移植14d后心脏灌注取脑制成石蜡切片,以苏木素伊红(HE)染色确定胶质瘤性质,SABC法免疫荧光染色显示NSCs对胶质瘤组织的定向迁移能力、分布规律及其迁移过程中存活和分化情况等。结果对侧半球组和对侧脑室组均可见移植的NSCs多沿着注射途径直线分布,并可见移植细胞沿着胼胝体,脑室,血管向对侧胶质瘤组织迁移。在胶质瘤与正常组织交界处可见激发出绿色荧光的阳性NSCs,鼠尾静脉组亦可见少量阳性细胞分布在胶质瘤组织周围。结论骨髓源NSCs对胶质瘤组织具有明显的趋化作用,能通过迁移准确定位于胶质瘤组织内。为以骨髓源NSCs为细胞载体的靶向治疗提供了实验依据。
[Abstract]:Objective to investigate the directional migration ability and distribution of bone marrow derived neural stem cells (NSCs) on gliomas. Methods Bone marrow stromal cells (BMSCs), basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) were isolated and purified from femur and tibia of male Wistar rats. The glioma model was established by stereotactic inoculation of C6 glioma cells in the basal ganglia of Wistar rats. After 7 days, 5-bromodeoxyuridine (5-bromodeoxyuridine) labeled RSCs was inoculated into the contralateral hemisphere, contralateral ventricle and caudal vein of the rat. After 14 days of NSCs transplantation, the brain was perfused to make paraffin sections, and the nature of gliomas was determined by hematoxylin eosin (HE) staining. SABC immunofluorescence staining showed the directional migration ability of NSCs to glioma tissues. Distribution law and survival and differentiation during migration. Results in the contralateral hemisphere group and contralateral ventricular group, most of the transplanted NSCs were distributed linearly along the injection route, and the transplanted cells migrated to the contralateral glioma tissue along the corpus callosum, ventricle and blood vessels. At the junction of glioma and normal tissues, a small number of positive cells could also be seen around the glioma tissues in the group of the tail vein of NSCs, mice, which excited green fluorescence. Conclusion NSCs derived from bone marrow has obvious chemotactic effect on glioma tissue and can be accurately located in glioma tissue by migration. It provides experimental basis for targeted therapy with bone marrow derived NSCs as cell carrier.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R329
本文编号：2411620
[Abstract]:Objective to investigate the directional migration ability and distribution of bone marrow derived neural stem cells (NSCs) on gliomas. Methods Bone marrow stromal cells (BMSCs), basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) were isolated and purified from femur and tibia of male Wistar rats. The glioma model was established by stereotactic inoculation of C6 glioma cells in the basal ganglia of Wistar rats. After 7 days, 5-bromodeoxyuridine (5-bromodeoxyuridine) labeled RSCs was inoculated into the contralateral hemisphere, contralateral ventricle and caudal vein of the rat. After 14 days of NSCs transplantation, the brain was perfused to make paraffin sections, and the nature of gliomas was determined by hematoxylin eosin (HE) staining. SABC immunofluorescence staining showed the directional migration ability of NSCs to glioma tissues. Distribution law and survival and differentiation during migration. Results in the contralateral hemisphere group and contralateral ventricular group, most of the transplanted NSCs were distributed linearly along the injection route, and the transplanted cells migrated to the contralateral glioma tissue along the corpus callosum, ventricle and blood vessels. At the junction of glioma and normal tissues, a small number of positive cells could also be seen around the glioma tissues in the group of the tail vein of NSCs, mice, which excited green fluorescence. Conclusion NSCs derived from bone marrow has obvious chemotactic effect on glioma tissue and can be accurately located in glioma tissue by migration. It provides experimental basis for targeted therapy with bone marrow derived NSCs as cell carrier.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R329
【参考文献】
相关期刊论文 前3条
1 鞠秀丽;黄志伟;侯怀水;时庆;段春红;沈柏均;;大鼠胎血与骨髓间充质干细胞分化能力的体外研究[J];临床血液学杂志;2006年05期
2 赵宇,陆应麟,乔群,杜芝燕,徐元基,戚可名;绿色荧光蛋白基因逆转录病毒载体转染人骨髓间充质干细胞及表达的研究[J];中华实验外科杂志;2004年01期
3 徐光锦,付新巧,杨华静,朱遂强,张苏明;小鼠MSCs分离体外扩增及向神经样细胞诱导分化的研究[J];中国康复;2005年01期
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