缺血后处理对兔睾丸缺血再灌注的影响

发布时间：2019-02-09 08:06

【摘要】：目的:探讨缺血后处理对不同缺血程度的兔睾丸缺血再灌注损伤的作用。方法:42只雄性大白兔随机分成对照组、缺血组(R1、R2、R3组)、后处理组(P1、P2、P3组),每组6只。缺血组、后处理组在超声监测下制成睾丸不同缺血程度模型:R1、P1组睾丸回声均匀,血流轻度减少;R2、P2组睾丸回声增粗,血流明显减少;R3、P3组睾丸出现片状或放射状低回声,血流信号消失。出现上述图像变化后,缺血组给予直接灌流,后处理组于恢复灌流前给予后处理措施,即再灌注30s/缺血30s,反复3次。再灌注前进行超声造影,3d后测定各组组织丙二醛(MDA)、超氧化物歧化酶(SOD)含量。HE染色后光镜下观察睾丸组织和病理学改变,并行Johnsen's评分和凋亡指数分析;电镜下观察睾丸组织超微结构。结果:再灌注前各造影参数速度参数(β)、峰值时间(TTP)、峰值基础强度差(PBD)、峰值减半时间(DT/2),R1与P1组比较均无显著差异(P0.05)、R2与P2组比较均无显著差异(P0.05)、R3与P3组无显影。MDA含量:R1与P1组比较有显著差异(P0.05),R2与P2组比较有显著差异(P0.05),R3与P3组比较无显著差异(P0.05)。SOD活性:R1与P1组比较有显著差异(P0.05),R2与P2组比较有显著差异(P0.05),R3与P3组比较无显著差异(P0.05)。Johnsen's评分:R1与P1组比较有显著差异(P0.05),R2与P2组比较无显著差异(P0.05),R3与P3组比较无显著差异(P0.05)。凋亡指数:R1与P1组比较有显著差异(P0.05),R2与P2组比较无显著差异(P0.05),R3与P3组比较无显著差异(P0.05)。电镜:P1组超微结构损伤程度较R1组轻,R2与P2组超微结构无明显差异,R3与P3组超微结构无明显差异。结论:缺血后处理可以减轻睾丸缺血再灌注损伤,但是受到缺血程度的影响,并且在病理学和生化学上的表现不一致。
[Abstract]:Aim: to investigate the effects of ischemic post-treatment on ischemic reperfusion injury in rabbit testis with different degrees of ischemia. Methods: Forty-two male white rabbits were randomly divided into control group, ischemia group (R1OR2R3 group) and post-treatment group (P1P2P2P3group) with 6 rabbits in each group. Ischemia group, post-treatment group under ultrasonic monitoring to make different degrees of testicular ischemia model: the testis of R1P 1 group with uniform testicular echo, slightly decreased blood flow, R2P 2 group testicular echo thickening, blood flow decreased significantly. In R 3 P 3 group, the testis showed flaky or radial hypoechoic echo, and the blood flow signal disappeared. After the changes of the above images, the ischemic group was given direct perfusion, and the post-treatment group was given post-treatment measures before reperfusion, that is, reperfusion 30s/ ischemia for 30 s, repeated 3 times. The content of malondialdehyde (MDA),) superoxide dismutase (SOD) was measured 3 days before reperfusion. The changes of testis and pathology were observed under light microscope after HE staining, and the Johnsen's score and apoptosis index were analyzed. The ultrastructure of testis was observed under electron microscope. Results: before reperfusion, the parameters of velocity (尾), peak (TTP), peak intensity difference, (PBD), peak half time (DT/2), no significant difference between R1 group and P1 group (P0.05), there was no significant difference between R1 group and P1 group (P0.05). There was no significant difference between R2 group and P2 group (P0.05), but there was no significant difference between R3 group and P3 group. MDA content was significantly different between R1 group and P1 group (P0.05), but significant difference between R2 group and P2 group (P0.05). There was no significant difference between R3 group and P3 group (P0.05). SOD activity; R1 and P1 group had significant difference (P0.05); R2 and P2 group had significant difference (P0.05); There was no significant difference between R3 group and P3 group (P0.05). Johnsen's score: there was significant difference between R1 and P1 group (P0.05), R2 and P2 group had no significant difference (P0.05), R3 and P3 group had no significant difference (P0.05). Apoptosis index: there was significant difference between R1 group and P1 group (P0.05), but there was no significant difference between R2 group and P2 group (P0.05), but there was no significant difference between R3 and P3 group (P0.05). Electron microscope: the degree of ultrastructural damage in P1 group was less than that in R1 group, but there was no significant difference between R2 group and P2 group, but there was no significant difference between R3 group and P3 group. Conclusion: Post-ischemic treatment can alleviate the ischemia reperfusion injury of testis, but it is affected by the degree of ischemia, and the pathological and biochemical manifestations are not consistent.
【作者单位】：福建医科大学附属协和医院超声科福建省超声医学研究所;
【基金】：福建省医学创新项目(2007-cx-11)~~
【分类号】：R697.22

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