建立动脉粥样硬化秽浊痰阻证动物模型的研究

发布时间：2019-02-10 18:44

【摘要】：目的:研究动脉粥样硬化秽浊痰阻证动物模型的建立。方法将60只载脂蛋白E基因敲除小鼠随机分为4组，即高脂饲料和干寒环境联合干预的模型组、高脂饲料对照组、干寒环境对照组和空白对照组。各组小鼠分别施加寒冷干燥环境和高胆固醇饲料等相关干预。喂养至第12周观察并记录小鼠体重、行为、大小便、上臂耐受力、舌象等生物学表征；处死后采集血液并检测血脂等生化指标；光学显微镜观察小鼠主动脉标本的细胞形态学的变化。将相关指标量化后各组之间进行比较。结果1.经过观察分析，联合干预高脂饲料和干寒环境的实验组小鼠所反映的秽浊痰阻证候表征最为突出；2.光镜观察主动脉细胞形态，各组小鼠动脉均可观察到粥样斑块和内膜的增厚；经过高胆固醇饲料和干寒环境对均内膜/管腔比值的作用分析，两种干预因素对内膜/管腔比值单独作用和交互作用的影响差异有统计学意义（P＜0.05）；即高脂饲料和干寒环境对内膜增厚有促进作用，使内膜/管腔比值增高，而两种因素同时联合干预的模型组小鼠动脉粥样硬化病变更为突出。结论置于干燥寒冷环境下喂予高胆固醇饲料的模型组小鼠的生物学表征和实验室检查结果表明此组小鼠的宏观、微观表现能够模拟动脉粥样硬化秽浊痰阻证，说明造模成功。
[Abstract]:Objective: to study the establishment of animal model of atherosclerotic turbid phlegm obstruction syndrome. Methods 60 apolipoprotein E knockout mice were randomly divided into 4 groups: model group with high fat diet and dry cold environment, high fat diet control group, dry cold environment control group and blank control group. Each group of mice were given cold and dry environment and high cholesterol diet and other related interventions. The body weight, behavior, defecation, upper arm tolerance and tongue image of the mice were observed and recorded at the 12th week of feeding, and the blood samples were collected and blood lipids were measured. The morphological changes of mouse aorta were observed by optical microscope. The relative indexes were compared after quantification. Result 1. Through observation and analysis, the experimental group mice with combined intervention of high fat diet and dry cold environment reflected the most prominent symptoms of the syndrome of turbidity and phlegm obstruction; 2. Atheromatous plaques and intimal thickening were observed in the arteries of each group. The effects of high cholesterol diet and dry cold environment on the ratio of mean intima to lumen were analyzed. There were significant differences between the two intervention factors on the ratio of intima to lumen (P < 0.05). That is to say, high fat diet and dry cold environment can promote intimal thickening and increase the ratio of intima to lumen, while the atherosclerotic lesion of the model group treated by two kinds of factors at the same time is more prominent. Conclusion the biological characteristics and laboratory results of the model group fed with high cholesterol diet in dry and cold environment show that the macroscopic and microscopic manifestations of this group can mimic the syndrome of atherosclerotic turbidity and phlegm obstruction, which indicates that the model is successful.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R-332;R259

【参考文献】