构建食管癌移植瘤模型及不同剂量六神丸干预后的微血管密度变化

发布时间：2019-03-03 14:13

【摘要】：背景:六神丸是传统中药复方,具有清热解毒,散结消肿止痛之功效。现代药理证明六神丸具有抗炎、镇痛、强心、抗病毒、抗肿瘤等作用,临床上常用于各类感染性疾病、恶性肿瘤的治疗。目的:探讨六神丸对食管癌移植瘤的抑瘤作用及对微血管密度、血管内皮生长因子表达的影响。方法:通过复制人食管癌裸鼠移植瘤模型,将48只裸鼠随机分为六神丸高剂量组、六神丸中剂量组、六神丸低剂量组、顺铂组、模型组、空白组,按照给药方案用药。用药20 d后处死动物,称量瘤体质量,计算抑瘤率;免疫组化检测瘤组织微血管密度、血管内皮生长因子的表达。结果与结论:六神丸高、中、低剂量组、顺铂组的移植瘤质量明显低于模型组,差异有显著性意义(P0.05)。六神丸高、中、低剂量各组移植瘤内微血管密度、血管内皮生长因子的表达明显低于模型组,但没有顺铂组明显。结果表明六神丸对食管癌移植瘤生长有明显抑制作用,通过下调肿瘤组织血管内皮生长因子表达活性、降低微血管密度,可能是六神丸抑瘤作用机制之一。
[Abstract]:Background: Liushen Pill is a traditional Chinese medicine compound, it has the effect of clearing heat and detoxifying, eliminating swelling and relieving pain. Modern pharmacology proves that Liushen Pill has anti-inflammatory, analgesic, heart-strengthening, anti-virus, anti-tumor and other effects. It is often used in the clinical treatment of various infectious diseases and malignant tumors. Aim: to investigate the effect of Liushen pill on tumor inhibition and microvessel density (MVD) and expression of vascular endothelial growth factor (VEGF) in transplanted tumor of esophageal carcinoma. Methods: 48 nude mice were randomly divided into three groups: high dose group, middle dose group, low dose group, cisplatin group, model group and blank group. The mice were randomly divided into three groups: high dose group, middle dose group, low dose group, cisplatin group, model group and blank group. After 20 days, the animals were killed, weighing the tumor body mass and calculating the tumor inhibition rate, and the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in the tumor tissue were detected by immunohistochemistry. Results & conclusion: the quality of transplanted tumor in high, middle, low dose group and cisplatin group was significantly lower than that in model group (P0.05). The microvessel density and the expression of vascular endothelial growth factor (VEGF) were significantly lower in the high, middle and low dose groups than those in the model group, but no significant difference was found in the cisplatin group. The results showed that Liushen pill could significantly inhibit the growth of transplanted tumor of esophageal carcinoma. It may be one of the mechanisms of Liushen pill to inhibit tumor growth by down-regulating the expression of vascular endothelial growth factor (VEGF) and reducing the microvessel density (MVD) of tumor tissue.
【作者单位】：湖南中医药大学;湖南中医药大学中西医结合学院;
【分类号】：R735.1;R-332

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