调胃承气汤证和小承气汤证兔动物模型的研制
发布时间：2019-03-04 13:04
【摘要】： 目的:研制出符合中医阳明腑实证中的调胃承气汤证和小承气汤证的兔动物模型,建立稳定的造模方法,并初步制定出该证的客观证候指标,探讨承气轻下法的治疗机理及意义。 方法:将105只白兔随机分为正常对照组(1组)、调胃承气汤证组(2组)、小承气汤证组(3组),2、3组再各分为病理模型组A、模型治疗组B、模型验证组C,即(1、2A、2B、2C、3A、3B、3C),共七组。正常对照组给以正常饲料喂养,自由饮水。调胃承气汤证组和小承气汤证组在实验前48小时分别给予拌有不同剂量次碳酸铋的饲料喂养2天,第2天禁水24小时。然后在自然状态下,调胃承气汤证组和小承气汤证组分别注射不同剂量的大肠杆菌内毒素。其中2A、3A组于攻毒前1小时、攻毒后2小时给予生理盐水,2B、3B组在攻毒前1小时、攻毒后2小时分别灌胃以调胃承气汤、小承气汤,2C、3C组则在造模的同时分别给予调胃承气汤和小承气汤,直至实验结束。正常对照组给予相应比例的生理盐水。在实验中,观测家兔的精神状态、呼吸、耳血管、体温、腹围等一般情况。于攻毒后6小时心脏采血,检测各项血液指标。随后处死动物,取肺、肝、肾、结肠等重要脏器作病理切片,光镜观察。 结果:正常对照组未见明显的变化。模型组家兔(2A、3A)体温明显升高,以2A组升高最多;2A、3A组家兔腹围显著增大,以3A组增大更明显。模型组的实验指标与正常对照组相比均有显著差异(P＜0.01或P＜0.05),其中WBC、NO、MDA和MMS含量有不同程度的增高,SOD含量明显下降;电解质中钠含量略有降低,钾含量则显著降低;全血粘度、血浆粘度升高,红细胞变形指数降低;TXB_2、TM值和PAI-1含量明显升高,同时t-PA含量明显降低。治疗组(2B、3B)、验证组(2C、3C)与模型组比较均有显著差异(P＜0.01或P＜0.05),与正常对照组比较,部分指标无显著差异,部分指标仍有显著差异(P＜0.01或P＜0.05)。病理形态大体观察:模型组(2A、3A)大部分家兔的肺脏表面可见点状或片状出血点,大肠膨隆胀气,肠管增粗,其余脏器未见明显病变。光镜观察:模型组家兔肺脏均有改变,2A组家兔的肺可见局灶性肺实变,3A组稍轻,可见肺泡间隔增宽,炎细胞浸润、充血。肠粘膜可见不同程度的炎细胞浸润、充血、水肿。肝脏也可见不同程度的炎细胞浸润,肾脏基本正常。治疗组与验证组家兔仅有轻微变化,或大致正常。 结论:采用次碳酸铋、禁水和大肠杆菌内毒素多因素联合造模方法,通过控制给药剂量,制作出不同的家兔病理模型,其主要症状、体征和病理变化符合《伤寒论》中调胃承气汤证、小承气汤证,故可以认为用此种造模方法所复制的调胃承气汤证、小承气汤证动物模型是成功的。2、调胃承气汤、小承气汤可以有效降低模型组家兔的体温,腹围、减轻其症状和体征,改善各种实验指标和病理改变,保护脏器组织。因此可以反证此种造模方法切实可靠,所制作的调胃承气汤证、小承气汤证家兔动物模型是成功的。
[Abstract]:Objective: to develop a rabbit model of regulating stomach-bearing Qi decoction and Xiao Cheng-Qi decoction in accordance with the syndrome of Yang-Ming fu organs of traditional Chinese medicine, and to establish a stable model-making method, and preliminarily draw up the objective syndrome index of this syndrome. Objective: to explore the therapeutic mechanism and significance of qi-bearing and light-lowering therapy. Methods: 105 white rabbits were randomly divided into normal control group (group 1), Tiaowei Chengqi decoction syndrome group (group 2) and Xiaoshengqi decoction group (group 3). The three groups were divided into pathological model group A, model treatment group B, model verification group C, that is, (1, 2 A, 2), and 2, 3 groups were divided into pathological model group A, model treatment group B, model verification group C, respectively. 2B, 2C, 3A, 3B, 3C), there were seven groups. The normal control group was fed with normal feed and drinking water freely. The two groups were fed with different dosages of bismuth carbonate for2 days and 24 hours respectively at 48 hours before the experiment in the Tiaowei Chengqi decoction group and Xiaochengqi decoction syndrome group. The rats were fed with different doses of bismuth carbonate for2 days and 24 hours respectively. Then, under the natural condition, different doses of E. coli endotoxin were injected into the syndrome group of Tiaowei Chengqi decoction and the group of Xiaochengqi decoction respectively. 2A, 3A group was given normal saline 1 hour before and 2 hours after challenge, and 3B group was given intragastric administration of Tiaowei Chengqi decoction, Xiaochengqi decoction, 2C2C, respectively 1 hour before and 2 hours after virus attack, and 2B, 3B group was administered intragastrically to Tiaowei Chengqi decoction, Xiaochengqi decoction, 2C, respectively. 3C group was given Tiaowei Chengqi decoction and XiaoChengQi decoction respectively at the same time, until the end of the experiment. The normal control group was given the corresponding proportion of normal saline. In the experiment, we observed the general state of mind, breathing, auricular vessels, body temperature, abdominal circumference and so on. The blood samples were collected from the heart at 6 hours after the attack, and the blood indexes were measured. Then the animals were killed, lung, liver, kidney, colon and other important organs were taken for pathological sections and observed under light microscope. Results: there was no obvious change in normal control group. The body temperature of rabbits in model group (2A, 3A) was significantly increased, especially in 2A group, and the abdominal circumference of 3A group was significantly increased, especially in 3A group. The experimental indexes in the model group were significantly different from those in the normal control group (P < 0. 01 or P < 0. 05). The contents of WBC,NO,MDA and MMS in the model group were increased to some extent, while the content of SOD was significantly decreased in the model group. Sodium content in electrolyte decreased slightly, potassium content decreased significantly, whole blood viscosity, plasma viscosity increased, erythrocyte deformability index decreased, TXB_2, TM value and PAI-1 content increased, and t-PA content decreased significantly. There were significant differences between the treatment group (2B, 3B) and the verification group (2C, 3C) compared with the model group (P < 0.01 or P < 0.05), and there were no significant differences in some indexes between the treatment group (2B, 3B) and the model group (P < 0.05). There were still significant differences in some indexes (P < 0.01 or P < 0.05). Pathological observation: in the model group (2A, 3A), punctate or lamellar bleeding spots were observed on the surface of the lungs, bulging of the large intestine, thickening of the intestinal tube, and no obvious pathological changes were observed in the other organs of the rabbits in the model group (2A, 3A). Light microscopic observation: there were changes in the lungs of rabbits in the model group. Focal consolidation could be seen in the lungs of the rabbits in the 2A group and slightly lighter in the 3A group. Widening of the alveolar septum, infiltration of inflammatory cells and hyperemia were observed in the pulmonary septum of the rabbits in the model group. Intestinal mucosa can see different degrees of inflammatory cell infiltration, congestion, edema. Different degrees of inflammatory cell infiltration can also be seen in the liver, and the kidney is basically normal. There were only slight changes, or generally normal, in the treatment group and the verification group. Conclusion: bismuth hypocarbonate, water ban and endotoxin of Escherichia coli were used to model rabbits, and different pathological models of rabbits were made by controlling the dosage of the drug, the main symptoms of which were the main symptoms. The physical signs and pathological changes accord with the syndrome of Tiaowei Chengqi decoction and XiaoChengQi decoction in the Treatise of febrile Syndrome, so it can be considered that the animal model of Tiaowei Chengqi decoction and Xiaochengqi decoction copied by this modeling method is successful. 2, Tiaowei Chengqi decoction, Xiaochengqi decoction can effectively reduce the body temperature, abdominal circumference, relieve its symptoms and signs, improve various experimental indexes and pathological changes, and protect organs and tissues in the model group. Therefore, this kind of modeling method can be proved to be effective and reliable, and the animal models of Tiaowei Chengqi decoction and Xiaochengqi decoction are successful.
【学位授予单位】：福建中医学院
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R-332
本文编号：2434290
[Abstract]:Objective: to develop a rabbit model of regulating stomach-bearing Qi decoction and Xiao Cheng-Qi decoction in accordance with the syndrome of Yang-Ming fu organs of traditional Chinese medicine, and to establish a stable model-making method, and preliminarily draw up the objective syndrome index of this syndrome. Objective: to explore the therapeutic mechanism and significance of qi-bearing and light-lowering therapy. Methods: 105 white rabbits were randomly divided into normal control group (group 1), Tiaowei Chengqi decoction syndrome group (group 2) and Xiaoshengqi decoction group (group 3). The three groups were divided into pathological model group A, model treatment group B, model verification group C, that is, (1, 2 A, 2), and 2, 3 groups were divided into pathological model group A, model treatment group B, model verification group C, respectively. 2B, 2C, 3A, 3B, 3C), there were seven groups. The normal control group was fed with normal feed and drinking water freely. The two groups were fed with different dosages of bismuth carbonate for2 days and 24 hours respectively at 48 hours before the experiment in the Tiaowei Chengqi decoction group and Xiaochengqi decoction syndrome group. The rats were fed with different doses of bismuth carbonate for2 days and 24 hours respectively. Then, under the natural condition, different doses of E. coli endotoxin were injected into the syndrome group of Tiaowei Chengqi decoction and the group of Xiaochengqi decoction respectively. 2A, 3A group was given normal saline 1 hour before and 2 hours after challenge, and 3B group was given intragastric administration of Tiaowei Chengqi decoction, Xiaochengqi decoction, 2C2C, respectively 1 hour before and 2 hours after virus attack, and 2B, 3B group was administered intragastrically to Tiaowei Chengqi decoction, Xiaochengqi decoction, 2C, respectively. 3C group was given Tiaowei Chengqi decoction and XiaoChengQi decoction respectively at the same time, until the end of the experiment. The normal control group was given the corresponding proportion of normal saline. In the experiment, we observed the general state of mind, breathing, auricular vessels, body temperature, abdominal circumference and so on. The blood samples were collected from the heart at 6 hours after the attack, and the blood indexes were measured. Then the animals were killed, lung, liver, kidney, colon and other important organs were taken for pathological sections and observed under light microscope. Results: there was no obvious change in normal control group. The body temperature of rabbits in model group (2A, 3A) was significantly increased, especially in 2A group, and the abdominal circumference of 3A group was significantly increased, especially in 3A group. The experimental indexes in the model group were significantly different from those in the normal control group (P < 0. 01 or P < 0. 05). The contents of WBC,NO,MDA and MMS in the model group were increased to some extent, while the content of SOD was significantly decreased in the model group. Sodium content in electrolyte decreased slightly, potassium content decreased significantly, whole blood viscosity, plasma viscosity increased, erythrocyte deformability index decreased, TXB_2, TM value and PAI-1 content increased, and t-PA content decreased significantly. There were significant differences between the treatment group (2B, 3B) and the verification group (2C, 3C) compared with the model group (P < 0.01 or P < 0.05), and there were no significant differences in some indexes between the treatment group (2B, 3B) and the model group (P < 0.05). There were still significant differences in some indexes (P < 0.01 or P < 0.05). Pathological observation: in the model group (2A, 3A), punctate or lamellar bleeding spots were observed on the surface of the lungs, bulging of the large intestine, thickening of the intestinal tube, and no obvious pathological changes were observed in the other organs of the rabbits in the model group (2A, 3A). Light microscopic observation: there were changes in the lungs of rabbits in the model group. Focal consolidation could be seen in the lungs of the rabbits in the 2A group and slightly lighter in the 3A group. Widening of the alveolar septum, infiltration of inflammatory cells and hyperemia were observed in the pulmonary septum of the rabbits in the model group. Intestinal mucosa can see different degrees of inflammatory cell infiltration, congestion, edema. Different degrees of inflammatory cell infiltration can also be seen in the liver, and the kidney is basically normal. There were only slight changes, or generally normal, in the treatment group and the verification group. Conclusion: bismuth hypocarbonate, water ban and endotoxin of Escherichia coli were used to model rabbits, and different pathological models of rabbits were made by controlling the dosage of the drug, the main symptoms of which were the main symptoms. The physical signs and pathological changes accord with the syndrome of Tiaowei Chengqi decoction and XiaoChengQi decoction in the Treatise of febrile Syndrome, so it can be considered that the animal model of Tiaowei Chengqi decoction and Xiaochengqi decoction copied by this modeling method is successful. 2, Tiaowei Chengqi decoction, Xiaochengqi decoction can effectively reduce the body temperature, abdominal circumference, relieve its symptoms and signs, improve various experimental indexes and pathological changes, and protect organs and tissues in the model group. Therefore, this kind of modeling method can be proved to be effective and reliable, and the animal models of Tiaowei Chengqi decoction and Xiaochengqi decoction are successful.
【学位授予单位】：福建中医学院
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R-332
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