脐带间充质干细胞支持脐血单个核细胞冻存复苏后的体外生长

发布时间：2019-03-12 17:06

【摘要】：目的:研究人脐带间充质干细胞(human umbilical cord mesenchymal stem cell,h UC-MSC)对冻存复苏后脐血单个核细胞(mononuclear cells,MNCs)体外生长的支持作用,以提高冷冻损伤后的造血细胞在体外的生存率。方法:用组织块培养法分离获得h UC-MSC,流式细胞仪检测其表面分子并诱导分化进行鉴定。分两组培养冻存复苏后的脐血MNCs:一组以h UC-MSC为滋养层共培养,另一组单独培养。光镜下观察两组MNCs密度。培养第10天,流式细胞仪检测两组造血细胞的百分比,并计数MNCs总数和造血细胞数进行对比。结果:成功分离培养获得h UC-MSC,符合各项鉴定标准。共培养组MNCs总数、CD45、CD14及CD19阳性造血细胞数均明显多于单培养组。结论:h UC-MSC可有效支持冻存复苏后脐血MNCs的体外生长。
[Abstract]:Aim: to study the effect of human umbilical cord mesenchymal stem cells (human umbilical cord mesenchymal stem cell,h UC-MSC) on the growth of cord blood mononuclear cells (mononuclear cells,MNCs) after cryopreservation and resuscitation, in order to improve the survival rate of hematopoietic cells after cryopreservation. Methods: h-UC-MSC, flow cytometry was used to detect the surface molecules and induce differentiation. Two groups of cryopreserved and resuscitated cord blood MNCs: were co-cultured with h-UC-MSC as trophoblast, and the other group was cultured alone. The density of MNCs was observed under light microscope. On the 10th day of culture, the percentage of hematopoietic cells in the two groups was measured by flow cytometry, and the total number of MNCs and the number of hematopoietic cells were compared. Results: h-UC-MSC, was successfully isolated and cultured in accordance with each identification standard. The total number of MNCs, CD45,CD14 and CD19 positive hematopoietic cells in co-culture group were significantly higher than those in single culture group. Conclusion: h-UC-MSC can effectively support the growth of cord blood MNCs after cryopreservation and resuscitation in vitro.
【作者单位】：湖北医药学院附属太和医院胚胎干细胞研究湖北省重点实验室;
【基金】：国家科技部重大专项子课题(2013ZX10001004-002-005)
【分类号】：R329.2

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