IFN-γ对孕期胚胎神经干细胞发育的影响
发布时间：2019-03-29 08:15
【摘要】： IFN-γ(gamma干扰素)是-种常见的感染因子,是炎症反应重要的组成部分。在孕母怀胎期间,不可避免受到各种感染因子的影响,而这些感染因子是否对胎儿神经系统的发育产生不良影响却少有文献报道。本文利用IFN-γ(浓度200U/ml)对大鼠体外培养胚胎16天的神经干细胞进行刺激,研究作为炎症反应重要组分的IFN-γ对胚胎神经干细胞的影响。 本文在第一部分首先建立了大鼠胚胎神经干细胞体外培养的模型。我们分离大鼠胚龄16天的神经干细胞,进行体外培养。这些神经干细胞能够表达特异性的标志nestin,当撤掉生长因子,加入血清时,能分化为神经元、星形胶质细胞和少突胶质细胞。我们以神经干细胞体外培养模型为基础对感染因子作用于胚胎神经干细胞的影响做出深入的分析研究。 在本文的第二部分,我们采用IFN-γ这一常见的感染因子,对大鼠的神经干细胞进行刺激,观察炎性分子对神经干细胞生长、分化的影响。我们发现,IFN-γ刺激2天能够显著上调神经干细胞表面MHC分子的表达。同时,IFN-γ能够促使神经干细胞向神经元和少突胶质细胞的分化,而抑制向星形胶质细胞的分化。 结论:我们成功建立了大鼠胚胎神经干细胞体外培养的模型。这种方法得到的神经干细胞90%以上表达神经干细胞特异性标志nestin,同时具有多向分化能力,能够分化成Rip阳性的少突胶质细胞,β-tubulinⅢ阳性的神经元和GFAP阳性的星形胶质细胞。IFN-γ作为一种感染因子,在高剂量下对胚胎神经干细胞具有毒性作用。IFN-γ能够上调神经干细胞MHCⅠ类和MHCⅡ类分子的表达,并且能够促进神经干细胞向神经元和少突胶质细胞的分化,降低向星形胶质细胞的分化。
[Abstract]:IFN- 纬 (gamma interferon) is a common infectious factor and an important component of inflammatory response. During pregnancy, it is inevitable to be affected by a variety of infection factors, but whether these infection factors have an adverse effect on the development of the fetal nervous system is rarely reported in the literature. IFN- 纬 (concentration 200U/ml) was used to stimulate neural stem cells (NSCs) of rat embryos cultured in vitro for 16 days. The effects of IFN- 纬, an important component of inflammatory reaction, on embryonic neural stem cells (NSCs) were studied. In the first part, the culture model of rat embryonic neural stem cells in vitro was established. Neural stem cells (NSCs) aged 16 days were isolated and cultured in vitro. These neural stem cells can express specific markers, nestin, when removed from the growth factor, added to the serum, can differentiate into neurons, astrocytes and oligodendrocytes. We studied the effects of infection factors on embryonic neural stem cells based on neural stem cell culture model in vitro. In the second part of this paper, we used IFN- 纬, a common infection factor, to stimulate rat neural stem cells and observe the effects of inflammatory molecules on the growth and differentiation of neural stem cells. We found that IFN- 纬 stimulation for 2 days could significantly up-regulate the expression of MHC molecules on the surface of neural stem cells. At the same time, IFN- 纬 can promote the differentiation of neural stem cells into neurons and oligodendrocytes, but inhibit the differentiation of neural stem cells to astrocytes. Conclusion: in vitro culture model of rat embryonic neural stem cells has been successfully established. The neural stem cells obtained by this method express more than 90% of the neural stem cell-specific marker nestin, and have the ability to differentiate into Rip-positive oligodendrocytes. 尾-tubulin 鈪，
本文编号：2449331
[Abstract]:IFN- 纬 (gamma interferon) is a common infectious factor and an important component of inflammatory response. During pregnancy, it is inevitable to be affected by a variety of infection factors, but whether these infection factors have an adverse effect on the development of the fetal nervous system is rarely reported in the literature. IFN- 纬 (concentration 200U/ml) was used to stimulate neural stem cells (NSCs) of rat embryos cultured in vitro for 16 days. The effects of IFN- 纬, an important component of inflammatory reaction, on embryonic neural stem cells (NSCs) were studied. In the first part, the culture model of rat embryonic neural stem cells in vitro was established. Neural stem cells (NSCs) aged 16 days were isolated and cultured in vitro. These neural stem cells can express specific markers, nestin, when removed from the growth factor, added to the serum, can differentiate into neurons, astrocytes and oligodendrocytes. We studied the effects of infection factors on embryonic neural stem cells based on neural stem cell culture model in vitro. In the second part of this paper, we used IFN- 纬, a common infection factor, to stimulate rat neural stem cells and observe the effects of inflammatory molecules on the growth and differentiation of neural stem cells. We found that IFN- 纬 stimulation for 2 days could significantly up-regulate the expression of MHC molecules on the surface of neural stem cells. At the same time, IFN- 纬 can promote the differentiation of neural stem cells into neurons and oligodendrocytes, but inhibit the differentiation of neural stem cells to astrocytes. Conclusion: in vitro culture model of rat embryonic neural stem cells has been successfully established. The neural stem cells obtained by this method express more than 90% of the neural stem cell-specific marker nestin, and have the ability to differentiate into Rip-positive oligodendrocytes. 尾-tubulin 鈪，
本文编号：2449331
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