芍药苷对血虚肝郁证模型大鼠海马cGMP含量及其合成与水解酶的影响

发布时间：2019-04-20 19:09

【摘要】：目的:研究血虚肝郁证大鼠海马c GMP含量及其合成酶s GCα2、s GCβ1和水解酶PDE2A、PDE5A、PDE9A m RNA表达与芍药苷的干预作用。方法:分为空白组、模型组、40mg/kg芍药苷组、20mg/kg芍药苷组,造模第1天开始给药,空白组、模型组给等量蒸馏水,其余各组给予相应药物,共造模21d。治疗结束后,取脑组织,剥离海马,采用格里斯法(Griess法)测定NO含量、放射免疫法(RIA)测定c GMP含量以及实时荧光定量聚合酶链反应法(RT-q PCR)检测s GCα2、s GCβ1、PDE2A、PDE5A、PDE9A m RNA的表达。结果:血虚肝郁证模型大鼠海马的NO、c GMP含量升高且s GCα2、s GCβ1、PDE2A、PDE5A m RNA的表达增加(P0.05,P0.01),40mg/kg芍药苷能够降低NO、c GMP含量(P0.01,P0.001)且下调其合成与分解酶的表达。结论:芍药苷能够降低血虚肝郁证模型大鼠海马NO、c GMP的含量,且下调s GCα2、s GCβ1、PDE2A、PDE5A m RNA的表达,其机制与芍药苷影响合成酶与水解酶的变化有关,从两个方面综合作用保持其含量的动态平衡。
[Abstract]:Aim: to study the effects of paeoniflorin on the expression of c GMP and its synthetase s GC 伪 2, s GC 尾 1 and hydrolase PDE2A,PDE5A,PDE9A m RNA in hippocampus of rats with blood deficiency and liver depression syndrome. Methods: they were divided into blank group, model group, 40mg/kg paeoniflorin group and 20mg/kg paeoniflorin group. The rats in the model group were given medicine from the first day, the blank group and the model group were given the same amount of distilled water, and the other groups were given the corresponding drugs. After treatment, the brain tissue was taken and the hippocampus was removed. The contents of NO, c-GMP and s GC 伪 2, s GC 尾 1, PDE 2A were measured by Griess method, (RIA) by radioimmunoassay, and s GC 伪 2, s GC 尾 1, PDE 2A by real-time fluorescence quantitative polymerase chain reaction (RT-q PCR). Expression of PDE5A,PDE9A m RNA. Results: the content of NO,c GMP increased and the expression of s GC 伪 2, s GC 尾 1, PDE 2 A, PDE 5A m RNA increased in hippocampus of rats with blood deficiency and liver stagnation syndrome (P 0.05, P0.01). 40mg/kg paeoniflorin could decrease the content of NO,c GMP (P 0.01, P < 0.01). P0.001) and down-regulated the expression of its synthetic and decomposing enzymes. Conclusion: paeoniflorin can decrease the content of NO,c GMP in hippocampus of rats with blood deficiency and liver stagnation, and down-regulate the expression of s GC 伪 2, s GC 尾 1, PDE 2A and PDE 5A m RNA. The mechanism is related to the effect of paeoniflorin on the changes of synthetase and hydrolase. It can keep the dynamic balance of its content from two aspects.
【作者单位】：北京中医药大学基础医学院;Department
【基金】：国家自然科学基金(No.81173569)~~
【分类号】：R-332;R285.5

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