微小牛蜱中肠内容物菌群结构分析

发布时间：2019-04-27 13:49

【摘要】：目的分析不同阶段的雌、雄微小牛蜱中肠内容物的细菌种群结构,为蜱及蜱传病防治提供科学依据。方法无菌收集微小牛蜱中肠内容物,以试剂盒提取细菌总DNA,通过通用引物PCR扩增16SrDNA V3区并进行DGGE电泳,选取9条优势条带进行回收克隆测序。结果微小牛蜱中肠优势菌9种,包括拉乌尔特立克次氏体,克雷伯菌,假单胞菌,生癌肠杆菌,阪崎肠杆菌,斯洛伐克立克次氏体,欧文氏菌,泛生菌,柯克斯氏体,其中,克雷伯菌、斯洛伐克立克次氏体、柯克斯氏体见于不同阶段的雌、雄微小牛蜱肠道;欧文氏菌、生癌肠杆菌仅见于雄成蜱,而泛生菌仅见于雌成蜱;阪崎肠杆菌只见于饱成蜱;雌蜱产卵后,肠道泛生菌、拉乌尔特立克次氏体优势消失;产卵5d后,蜱肠道菌群结构基本稳定。结论不同阶段的雌、雄微小牛蜱中肠内容物的细菌种群结构存在一定程度的差别。
[Abstract]:Aim to analyze the bacterial population structure of midgut contents of female and male ticks at different stages in order to provide scientific basis for ticks and ticks-borne disease control. Methods 16SrDNA V3 region was amplified by universal primer PCR and DGGE electrophoresis was carried out. Nine dominant bands were selected and cloned and sequenced. The total DNA, of bacteria was extracted from the midgut contents of Boaphysalis minutum by asepsis, and the bacterial total PCR was used to amplify the V3 region by universal primer PCR. Results there were 9 species of dominant bacteria in midgut of Boaphysalis microphylla, including Ramultriktsia, Klebsiella, Pseudomonas, Enterobacter carcinogenes, Enterobacter sakazakii, Rickettsia Slovakia, Irvine, Panerophyte, Coxosite, among them, Klebsiella spp., Rickettbody, Kirschner's body in different stages of female, male calf ticks intestinal tract; Erwinia, Enterobacter carcinogenes was found only in male ticks, while Panthenophyte was only found in female adult ticks, Enterobacter sakazakii was only found in fulminating ticks, after laying eggs in female ticks, the dominance of intestinal ubiquium and Raulteketzite body disappeared. After laying eggs for 5 days, the structure of intestinal flora of ticks was basically stable. Conclusion there are some differences in the bacterial population structure of midgut contents between male and female in different stages.
【作者单位】：湖南农业大学动物医学院;信阳农林学院动物科学系;
【基金】：国家自然科学基金面上项目(No.31372431)
【分类号】：R384.4

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