近红外荧光探针及其在免疫分析中的应用

发布时间：2019-04-28 16:38

【摘要】：综合归纳了有机荧光分子、量子点、稀土配合物及单壁碳纳米管共4类重要近红外荧光标记探针的性质、特征,及其在光学性能改进、信号增强等方面的最新发展,分析评述了其在环境污染物及临床诊断标志分子免疫分析中的应用,展望了基于该类探针的免疫层析法在食源性致病菌快速检测中的应用潜能。相对于发射光光谱位于紫外及可见光区的信号分子,近红外荧光探针因其具有信噪比高、组织穿透力强、对基体损伤小等突出的优势,而在生物分析领域备受瞩目。随着化学合成技术的不断发展及新型荧光材料的持续发掘,近年来近红外荧光探针日益丰富,并在无损分析、免疫检测和生物造影等领域被广泛应用。
[Abstract]:The properties and characteristics of four kinds of important near infrared fluorescence labeled probes including organic fluorescence molecules, quantum dots, rare earth complexes and single wall carbon nanotubes, as well as their latest developments in optical performance improvement and signal enhancement, are summarized in this paper, as well as their recent developments in organic fluorescence molecules, quantum dots, rare earth complexes and single wall carbon nanotubes. Its application in molecular immunoassay of environmental pollutants and clinical diagnostic markers was analyzed and reviewed. The application potential of immunochromatographic method based on this kind of probe in the rapid detection of food-borne pathogenic bacteria was prospected. Compared with the signal molecules located in the ultraviolet and visible regions of the emission spectrum, the near infrared fluorescence probes have attracted much attention in the field of biological analysis because of their outstanding advantages such as high signal-to-noise ratio, strong tissue penetration and little damage to the matrix. With the continuous development of chemical synthesis technology and the continuous discovery of new fluorescent materials, the near infrared fluorescence probes are becoming more and more abundant in recent years, and have been widely used in the fields of nondestructive analysis, immunoassays and biomatography.
【作者单位】：厦门市产品质量监督检验院;北京出入境检验检疫局;长春理工大学化学与环境工程学院;河北省食品检验研究院;淮安出入境检验检疫局;临沂出入境检验检疫局;威海出入境检验检疫局;
【基金】：国家质检总局科技项目(2013IK144) 国家质检总局公益性行业科研专项(201410049) 福建省自然科学基金项目(2014J01118)
【分类号】：R392

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