类几丁质酶3蛋白1在申克孢子丝菌刺激巨噬细胞的表达和作用研究

发布时间：2019-05-10 09:12

【摘要】：目的探讨巨噬细胞类几丁质酶3蛋白1在申克孢子丝菌刺激后的表达和作用研究。方法通过测定巨噬细胞与申克孢子丝菌共孵育时杀菌率,使用实时荧光定量PCR检测不同时间点巨噬细胞的类几丁质酶3蛋白1mRNA表达水平,同时体外实验检测类几丁质酶3蛋白1对申克孢子丝菌的抑菌作用,同时采用空白对照和白念珠菌阳性对照比较两者的差异。结果巨噬细胞可吞噬杀灭申克孢子丝菌分生孢子,也可吞噬阳性对照白念珠菌酵母相细胞。巨噬细胞类几丁质酶3蛋白1mRNA表达水平在申克孢子丝菌组和空白对照组无明显差别,作用于阳性对照的白念珠菌组的巨噬细胞类几丁质酶3蛋白1mRNA表达水平明显上调。体外实验显示类几丁质酶3蛋白1对申克孢子丝菌分生孢子的无明显抑制作用,对白念珠菌繁殖却有较强的抑制作用。结论申克孢子丝菌未能诱导巨噬细胞表达类几丁质酶3蛋白1抑制其增殖从而逃逸清除,而白念珠菌感染可上调巨噬细胞类几丁质酶3蛋白1表达从而抑制其增殖减轻感染,这可能是申克孢子丝菌特有的致病作用机制之一。
[Abstract]:Objective to investigate the expression and effect of macrophage chitinase 3 protein 1 stimulated by Sporidium schenckii. Methods the bactericidal rate of macrophages incubated with Sporidium Schenckii was measured, and the expression of chitinase 3 protein 1mRNA in macrophages at different time points was detected by real-time fluorescence quantitative PCR. At the same time, the bacteriostatic effect of chitinase 3 protein 1 on Sporidium schenckii was detected in vitro, and the difference between blank control and candida albicans positive control was compared. Results macrophages could kill the conidia of Sporidium Schenckii and the yeast phase cells of Saccharomyces albicans in the positive control. There was no significant difference in the expression level of chitinase 3 protein 1mRNA between the Schenckosporium group and the blank control group, but the expression level of the macrophage chitinase 3 protein 1mRNA in the positive control group was significantly up-regulated in the Candida albicans group. In vitro experiments showed that chitinase 3 protein 1 had no obvious inhibitory effect on conidia of Sporidium schenckii, but had a strong inhibitory effect on the propagation of candida albicans. Conclusion the expression of chitinase-3 protein 1 in macrophages could not be induced by Sporosporidium schenckii to inhibit its proliferation and escape clearance. However, Candida albicans infection could up-regulate the expression of chitinase-3 protein-1 in macrophages and inhibit its proliferation and alleviate the infection. This may be one of the unique pathogenic mechanisms of Sporidium Schenckii.
【作者单位】：中山大学附属第三医院皮肤病科;中山大学孙逸仙纪念医院皮肤病科;广东省皮肤性病防治中心;
【基金】：国家自然科学基金面上项目(No.81371746) 广东省省级科技计划项目(No.2013B010404013)~~
【分类号】：R392

【共引文献】