切除85%肝与药物诱导大鼠急性肝衰竭模型的对比研究

发布时间：2019-05-19 13:26

【摘要】： 目的: 比较外科手术与药物诱导建立大鼠急性肝衰竭(ALF)模型的方法,探讨一种制备稳定的ALF模型的方法。建立与人急性肝衰竭病理过程、生化改变相似,实用性、重复性好的动物模型,为研究急性肝衰竭发生的病理生理机制、药物筛选及疗效评价提供适宜的模型。方法: 将SD大鼠60只随机分为二组: (1)手术切除诱导组(A组),切除约85 %肝脏组织,术中经左肾静脉注入5%葡萄糖氯化钠溶液10ml/kg体重;(2)药物诱导组(B组),将四氯化碳(CCl4)与等量花生油混合成50%溶液,按0.9ml/ kg腹腔注射。观察手术死亡率、模型建立后2 4 h大鼠存活率、血谷丙转氨酶(ALT)、天门冬氨酸氨基转移酶(AST)、白蛋白(ALB)、碱性磷酸酶(ALP)、血氨(NH3)、总胆红素(TBIL)、凝血酶原时间(PT)和血糖(BG)等指标。并取不同时间点的肝脏组织行病理学检查。结果: 手术切除组与药物组的大鼠建模成功后24h存活率分别为0%,30%,手术切除组ALT、AST、ALP、NH3、PT的水平要显著高于药物组(P0.05),而TBIL、ALB和BG水平低于药物诱导组,但差异无显著性(P0.05)。A组12h病理显示肝脏损害不严重;B组72h病理显示肝脏大片坏死,损害严重。结论: 通过改进的85%肝切除术可建立较理想的大鼠ALF模型,术中经左肾静脉补液可减少手术死亡率。采用手术切除法制备的ALF模型值得推广应用,具有快速、简便、易控的特点,可以用于更深一步的研究和应用。
[Abstract]:Objective: to compare the methods of establishing (ALF) model of acute liver failure in rats by surgery and drug induction, and to explore a method of establishing stable ALF model. An animal model similar to the pathological process and biochemical changes of human acute liver failure was established, which provided a suitable model for the study of pathophysiological mechanism, drug screening and curative effect evaluation of acute liver failure. Methods: 60 SD rats were randomly divided into two groups: (1) resection induction group (group A), resection of about 85% liver tissue, intraoperative injection of 5% glucose sodium chloride solution 10ml/kg body weight through left renal vein; (2) in the drug induction group (group B), carbon tetrachloride (CCl4) was mixed with the same amount of peanut oil into 50% solution and injected intraperitoneally according to 0.9ml/ kg. The survival rate of 24 hours after the establishment of the model was observed. (ALT), aspartate aminotransferase (AST), albumin (ALB), alkaline phosphatase (ALP), blood ammonia (NH3) and total bilirubin (TBIL), were observed. Prothrombin time (PT) and blood glucose (BG) were measured. The liver tissues at different time points were examined by pathology. Results: the 24 h survival rates of the rats in the resection group and the drug group were 0% and 30% respectively. The level of ALT,AST,ALP,NH3,PT in the resection group was significantly higher than that in the drug group (P 0.05), while TBIL, was significantly higher than that in the drug group. The levels of ALB and BG in the drug-induced group were lower than those in the drug-induced group, but there was no significant difference (P 0.05). 12 h pathology in the). A group showed that the liver damage was not serious. In group B, 72 h pathology showed massive necrosis of the liver and serious damage. Conclusion: an ideal rat model of ALF can be established by improved 85% hepatectomy, and intraoperative infusion of fluid through left renal vein can reduce the operative mortality. The ALF model prepared by surgical resection is worthy of popularization and application, which is rapid, simple and easy to control, and can be used for further research and application.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R575.3;R-332

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