IL-2预孵育影响G-CSF动员的小鼠脾淋巴细胞增殖及分化的实验研究
发布时间：2019-06-11 23:04
【摘要】： 目的: 探讨IL-2预孵育对G-CSF动员后的小鼠脾淋巴细胞功能的影响,从而为减轻异基因造血干细胞移植中急性GVHD提供一种新的方法 方法: 1,予rhG-CSF(0.2μg/g.d)动员的C57BL/6小鼠脾细胞和C57BL/6小鼠na(1|¨)veCD4+T细胞,BALB/c小鼠异基因抗原分别对其进行刺激,测定C57BL/6小鼠脾细胞和C57BL/6小鼠na(1|¨)veCD4+T细胞增殖能力和分化方向 2,用IL-2(50U/mL)预孵育C57BL/6小鼠脾细胞,检测脾细胞SOCS-3基因的表达变化 3,用IL-2(50U/mL)预孵育C57BL/6小鼠脾细胞和C57BL/6小鼠na(1|¨)veCD4+T细胞,然后BALB/c小鼠异基因抗原分别对其进行刺激,测定C57BL/6小鼠脾细胞和C57BL/6小鼠na(1|¨)veCD4+T细胞免疫增殖能力和分化方向 4,IL-2(50U/mL)预孵育经rhG-CSF(0.25μg/g.d)动员的C57BL/6小鼠脾细胞和C57BL/6小鼠na(1|¨)veCD4+T细胞,然后BALB/c小鼠异基因抗原分别对其进行刺激,测定动员后的C57BL/6小鼠脾细胞和C57BL/6小鼠na(1|¨)veCD4+T细胞免疫增殖能力和分化方向 结果: 1,经过一定浓度rhG-CSF动员的C57BL/6小鼠脾细胞和C57BL/6小鼠na(1|¨)veCD4+T细胞对同种异基因抗原免疫增殖能力减弱,并向Th2方向分化 2,C57BL/6小鼠脾细胞经IL-2预孵育后,SOCS-3的表达升高,在6h达到高峰 3,C57BL/6小鼠脾细胞和C57BL/6小鼠na(1|¨)veCD4+T细胞经IL-2预孵育后对同种异基因抗原免疫增殖能力明显减弱并向Th2方向分化 4,rhG-CSF动员的C57BL/6小鼠脾细胞和C57BL/6小鼠na(1|¨)veCD4+T细胞经IL-2预孵育后对同种异基因抗原免疫增殖能力明显减弱并向Th2方向分化,两者合用在诱导T淋巴细胞免疫耐受上有协同效应。
[Abstract]:Objective: to investigate the effect of IL-2 pre-incubation on the function of spleen lymphocytes in mice mobilized with G-CSF, so as to provide a new method for reducing acute GVHD in allogenic hematopoietic stem cell transplantation: 1, spleen cells of C57BL/6 mice mobilized by rhG-CSF (0.2 渭 g / g 路d) and na (1) veCD4 T cells of C57BL/6 mice. The spleen cells of BALB/c mice were stimulated by allogenic antigen. The proliferation and differentiation direction of na (1) veCD4 T cells in C57BL/6 mice and C57BL/6 mice were measured. The spleen cells of C57BL/6 mice were preincubated with IL-2 (50U/mL), and the expression of SOCS-3 gene in spleen cells was detected. Spleen cells of C57BL/6 mice and na (1) veCD4 T cells of C57BL/6 mice were preincubated with IL-2 (50U/mL), and then stimulated by allogenic antigen of BALB/c mice. The immune proliferation ability and differentiation direction of spleen cells of C57BL/6 mice and C57BL/6 mice na (1 / 1) veCD4 T cells were measured. IL-2 (50U/mL) preincubated C57BL/6 mouse spleen cells and C57BL/6 mouse na (1) veCD4 T cells mobilized by rhG-CSF (0.25 渭 g / g 路d), and then BALB/c mouse allogenic antigens stimulated them respectively. the immune proliferation ability and differentiation direction of mobilized C57BL/6 mouse spleen cells and C57BL/6 mouse na (1) veCD4 T cells were measured. The spleen cells of C57BL/6 mice mobilized by a certain concentration of rhG-CSF and na (1) veCD4 T cells of C57BL/6 mice attenuated the immune proliferation ability of allogenic antigen and differentiated into Th2 direction 2. The expression of SOCS-3 increased after preincubated with IL-2 and reached the peak of 3 at 6 h. Spleen cells of C57BL/6 mice and na (1) veCD4 T cells of C57BL/6 mice were preincubated with IL-2 and their immune proliferation ability to allogenic antigen was significantly weakened and differentiated into Th2 direction. Spleen cells of C57BL/6 mice mobilized by RHG and na (1) veCD4 T cells of C57BL/6 mice were significantly attenuated and differentiated into Th2 direction after IL-2 preincubation. The combination of the two had synergistic effect on inducing immune tolerance of T lymphocytes.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392
本文编号：2497527
[Abstract]:Objective: to investigate the effect of IL-2 pre-incubation on the function of spleen lymphocytes in mice mobilized with G-CSF, so as to provide a new method for reducing acute GVHD in allogenic hematopoietic stem cell transplantation: 1, spleen cells of C57BL/6 mice mobilized by rhG-CSF (0.2 渭 g / g 路d) and na (1) veCD4 T cells of C57BL/6 mice. The spleen cells of BALB/c mice were stimulated by allogenic antigen. The proliferation and differentiation direction of na (1) veCD4 T cells in C57BL/6 mice and C57BL/6 mice were measured. The spleen cells of C57BL/6 mice were preincubated with IL-2 (50U/mL), and the expression of SOCS-3 gene in spleen cells was detected. Spleen cells of C57BL/6 mice and na (1) veCD4 T cells of C57BL/6 mice were preincubated with IL-2 (50U/mL), and then stimulated by allogenic antigen of BALB/c mice. The immune proliferation ability and differentiation direction of spleen cells of C57BL/6 mice and C57BL/6 mice na (1 / 1) veCD4 T cells were measured. IL-2 (50U/mL) preincubated C57BL/6 mouse spleen cells and C57BL/6 mouse na (1) veCD4 T cells mobilized by rhG-CSF (0.25 渭 g / g 路d), and then BALB/c mouse allogenic antigens stimulated them respectively. the immune proliferation ability and differentiation direction of mobilized C57BL/6 mouse spleen cells and C57BL/6 mouse na (1) veCD4 T cells were measured. The spleen cells of C57BL/6 mice mobilized by a certain concentration of rhG-CSF and na (1) veCD4 T cells of C57BL/6 mice attenuated the immune proliferation ability of allogenic antigen and differentiated into Th2 direction 2. The expression of SOCS-3 increased after preincubated with IL-2 and reached the peak of 3 at 6 h. Spleen cells of C57BL/6 mice and na (1) veCD4 T cells of C57BL/6 mice were preincubated with IL-2 and their immune proliferation ability to allogenic antigen was significantly weakened and differentiated into Th2 direction. Spleen cells of C57BL/6 mice mobilized by RHG and na (1) veCD4 T cells of C57BL/6 mice were significantly attenuated and differentiated into Th2 direction after IL-2 preincubation. The combination of the two had synergistic effect on inducing immune tolerance of T lymphocytes.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392
【参考文献】
相关期刊论文 前2条
1 常英军,赵翔宇,黄晓军;rhG-CSF体内诱导造血干细胞移植供者外周血T细胞免疫耐受的初步研究[J];中国实验血液学杂志;2005年01期
2 黄文荣;王立生;高春记;鲁茁壮;王华;段海峰;达万明;;rhG-CSF动员对供者T细胞增殖和细胞毒的影响[J];中国实验血液学杂志;2006年05期
，本文编号：2497527
本文链接：https://www.wllwen.com/yixuelunwen/shiyanyixue/2497527.html
最近更新
教材专著
