慢性肾功能不全患者和肾移植受者外周血调节性T细胞的表达
发布时间：2019-06-19 20:14
【摘要】： 调节性T细胞是一种免疫调节细胞,其主要以主动方式诱导和维持机体外周免疫耐受,调节机体免疫平衡,多项研究发现其在预防和抑制自身免疫性疾病、炎症、变态反应和移植排斥中有着非常重要的作用。 慢性肾功能衰竭的发展过程可以随着肾脏受损的逐步加重而分为下列四个时期:代偿期、失代偿期、衰竭期和尿毒症期。慢性肾功能不全常并发免疫功能障碍,而且以细胞免疫异常为主。调节性T细胞(Treg)是具有免疫抑制功能的一群T细胞,在维持机体免疫自稳、免疫耐受和防止自身免疫性疾病的发生方面具有十分重要的作用。我们利用流式细胞术检测了50例慢性肾功能不全患者外周血CD4+CD127-Treg、CD4+ CD25+CD127- Treg的表达水平,并与健康人群作对比,以探讨其在不同肾功能状态下的变化。研究发现慢性肾功能不全各组患者外周血CD4+CD127-Treg占CD4+T细胞的比例显著低于健康对照组,而CD4+CD25+CD127-Treg占CD4+T细胞的比例较健康对照组无显著性差异,代偿期患者CD4+T占淋巴细胞的比例跟健康对照组相比,没有统计学差异,其余各期均显著高于健康对照组。提示慢性肾功能不全患者存在免疫功能异常。患者CD4+T占淋巴细胞的比例,CD4+CD127-Treg、CD4+CD25+CD127-Treg占CD4+T细胞的比例与血清BUN和Cr值之间没有相关性。 我们通过测定13例维持性血液透析患者血透前、血透后外周血CD4+T细胞占淋巴细胞的比例及CD4+CD127-Treg、CD4+CD25+CD127-Treg占CD4+T细胞的比例,探讨血液透析前后患者体内CD4+CD127-Treg、CD4+CD25+CD127-Treg的表达水平及意义。观察发现维持性血液透析患者血透前CD4+T细胞占淋巴细胞的比例高于健康对照组,CD4+CD127-Treg、CD4+CD25+CD127-Treg占CD4+T细胞的比例低于健康对照组,血透后CD4+T的比例下降,CD4+CD25+CD127-Treg的比例有所上升。提示维持性血液透析患者外周血CD4+T数量增高,CD4+CD127-Treg、CD4+CD25+CD127-Treg数量降低,免疫功能紊乱,而血透有利于改善机体的免疫功能。 肾移植是目前尿毒症患者理想、经济,并可明显提高生活质量的一种治疗方法,但是排斥反应与长期应用免疫抑制剂的副作用,包括免疫抑制引起的感染、肿瘤发生率的增加等严重影响着移植物及受者的存活。如何控制器官移植后排斥反应,诱发宿主对移植物的免疫耐受,一直是移植免疫学领域研究的热点及难题。在体内外通过抗原非特异性或特异性扩增,下调效应性免疫细胞的功能并削弱其增殖能力,来抑制或控制机体免疫应答的程度。我们将肾移植受者分为慢性排斥组和稳定组,通过测定其外周血CD4+CD127-Treg、CD4+CD25+CD127-Treg水平,并与健康对照组作对比,探讨其在诱导异源器官移植耐受方面的重要作用,为克服排斥反应提供一些参考。 研究发现慢性排斥组淋巴细胞中CD4+T的比例明显高于健康对照组和稳定组,有统计学差异。而稳定组CD4+T细胞的比例低于慢排组,与健康对照组相比无显著差异。慢性排斥组CD4+CD127-Treg占CD4+T细胞的比例明显低于健康对照组和稳定组,有统计学差异;稳定组CD4+CD127-Treg占CD4+T细胞的比例较健康对照组稍低但无统计学差异。慢性排斥组CD4+CD25+CD127-Treg占CD4+T细胞的比例低于健康对照组且有统计学差异,与稳定组相比无统计学差异。稳定组CD4+CD25+CD127-Treg占CD4+T细胞的比例,与健康对照组相比无统计学差异。提示如果促进Treg的发育和增殖,将能有效地增强机体的免疫耐受,降低免疫抑制剂的用量。
[Abstract]:The regulatory T cell is an immunomodulatory cell, which mainly induces and maintains the peripheral immune tolerance of the body in an active manner, regulates the immune balance of the body, and has been found to be used for preventing and inhibiting the autoimmune diseases and inflammation, Allergy and transplant rejection have a very important role. The development of chronic renal failure can be divided into the following four periods with the gradual increase of the kidney damage: the compensation period, the decompensation stage, the failure period and the urinary toxicity. The chronic renal function is not always accompanied with immune dysfunction, and is abnormal in cellular immunity. The primary regulatory T cell (Treg) is a group of T cells with immunosuppressive function, which is of great importance in the maintenance of immune homeostasis, immune tolerance and the prevention of autoimmune diseases. The expression level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in the peripheral blood of 50 patients with chronic renal insufficiency was detected by flow cytometry and compared with the healthy population to investigate the expression level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in 50 patients with chronic renal insufficiency. The results showed that the ratio of CD4 + CD127-Treg in the peripheral blood of the patients with chronic renal insufficiency was significantly lower than that in the healthy control group, while the proportion of CD4 + CD25 + CD127-Treg in the CD4 + T cells was significantly lower than that in the healthy control group. The difference is that the rest of the period is significantly higher than that of the healthy pair Group. It is suggested that the immune function in patients with chronic renal insufficiency Abnormality. The ratio of CD4 + T in the patient to the lymphocytes, CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg, or CD4 + T cells, is not associated with the serum BUN and Cr values The expression of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in patients before and after hemodialysis was discussed by measuring the ratio of CD4 + T cells in peripheral blood and CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg to CD4 + T cells before and after hemodialysis. The results showed that the ratio of CD4 + T cells to lymphocytes in the patients with maintenance hemodialysis was higher than that of healthy control group, and the ratio of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg to CD4 + T cells was lower than that of healthy control group. The ratio of CD4 + T after hemodialysis was lower, and the ratio of CD4 + CD25 + CD127-Treg was lower. The number of CD4 + T, CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in peripheral blood of patients with maintenance hemodialysis were increased, and the number of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg decreased and the immune function was disordered. Kidney transplantation is an ideal and economical method for uremic patients and can obviously improve the quality of life, but the side effects of rejection and long-term application of immunosuppressants, including the maintenance-free The infection, the increase of the incidence of the tumor, etc. caused by the inhibition of the epidemic are seriously affected by the shift. The survival of the plant and the recipient. How to control the rejection after organ transplantation and to induce the immune tolerance of the host to the graft has been the field of immunology It is a hot topic and a difficult problem to study. In vivo, the function of effector immune cells is down-regulated and its proliferation ability is reduced by non-specific or specific amplification of the antigen, so as to inhibit or control the proliferation of the immune cells. The level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in peripheral blood of the renal transplant recipients was measured and compared with the healthy control group. The results showed that the ratio of CD4 + T in the lymphocytes of the chronic rejection group was significantly higher than that of the healthy control group. and the proportion of the stable group CD4 + T cells is lower than that of the slow-row group and the stable group, Compared with the control group, the proportion of CD4 + CD127-Treg in the chronic rejection group was significantly lower than that in the healthy control group and the stable group, and the proportion of the CD4 + CD127-Treg in the stable group was significantly lower than that of the healthy control group and the stable group, and the ratio of the CD4 + CD127-Treg in the stable group to the CD4 + T cell was healthy. The proportion of CD4 + CD25 + CD127-Treg in chronic rejection group was lower than that of healthy control group and there was no statistical difference. The ratio of CD4 + CD25 + CD127-Treg in the stable group to CD4 + T cells and health There is no statistical difference in the control group. It is suggested that the immune tolerance of the body can be effectively enhanced if the development and proliferation of the Treg are promoted.
【学位授予单位】：广西师范大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392.12;R692.5;R699
本文编号：2502610
[Abstract]:The regulatory T cell is an immunomodulatory cell, which mainly induces and maintains the peripheral immune tolerance of the body in an active manner, regulates the immune balance of the body, and has been found to be used for preventing and inhibiting the autoimmune diseases and inflammation, Allergy and transplant rejection have a very important role. The development of chronic renal failure can be divided into the following four periods with the gradual increase of the kidney damage: the compensation period, the decompensation stage, the failure period and the urinary toxicity. The chronic renal function is not always accompanied with immune dysfunction, and is abnormal in cellular immunity. The primary regulatory T cell (Treg) is a group of T cells with immunosuppressive function, which is of great importance in the maintenance of immune homeostasis, immune tolerance and the prevention of autoimmune diseases. The expression level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in the peripheral blood of 50 patients with chronic renal insufficiency was detected by flow cytometry and compared with the healthy population to investigate the expression level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in 50 patients with chronic renal insufficiency. The results showed that the ratio of CD4 + CD127-Treg in the peripheral blood of the patients with chronic renal insufficiency was significantly lower than that in the healthy control group, while the proportion of CD4 + CD25 + CD127-Treg in the CD4 + T cells was significantly lower than that in the healthy control group. The difference is that the rest of the period is significantly higher than that of the healthy pair Group. It is suggested that the immune function in patients with chronic renal insufficiency Abnormality. The ratio of CD4 + T in the patient to the lymphocytes, CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg, or CD4 + T cells, is not associated with the serum BUN and Cr values The expression of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in patients before and after hemodialysis was discussed by measuring the ratio of CD4 + T cells in peripheral blood and CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg to CD4 + T cells before and after hemodialysis. The results showed that the ratio of CD4 + T cells to lymphocytes in the patients with maintenance hemodialysis was higher than that of healthy control group, and the ratio of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg to CD4 + T cells was lower than that of healthy control group. The ratio of CD4 + T after hemodialysis was lower, and the ratio of CD4 + CD25 + CD127-Treg was lower. The number of CD4 + T, CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in peripheral blood of patients with maintenance hemodialysis were increased, and the number of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg decreased and the immune function was disordered. Kidney transplantation is an ideal and economical method for uremic patients and can obviously improve the quality of life, but the side effects of rejection and long-term application of immunosuppressants, including the maintenance-free The infection, the increase of the incidence of the tumor, etc. caused by the inhibition of the epidemic are seriously affected by the shift. The survival of the plant and the recipient. How to control the rejection after organ transplantation and to induce the immune tolerance of the host to the graft has been the field of immunology It is a hot topic and a difficult problem to study. In vivo, the function of effector immune cells is down-regulated and its proliferation ability is reduced by non-specific or specific amplification of the antigen, so as to inhibit or control the proliferation of the immune cells. The level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in peripheral blood of the renal transplant recipients was measured and compared with the healthy control group. The results showed that the ratio of CD4 + T in the lymphocytes of the chronic rejection group was significantly higher than that of the healthy control group. and the proportion of the stable group CD4 + T cells is lower than that of the slow-row group and the stable group, Compared with the control group, the proportion of CD4 + CD127-Treg in the chronic rejection group was significantly lower than that in the healthy control group and the stable group, and the proportion of the CD4 + CD127-Treg in the stable group was significantly lower than that of the healthy control group and the stable group, and the ratio of the CD4 + CD127-Treg in the stable group to the CD4 + T cell was healthy. The proportion of CD4 + CD25 + CD127-Treg in chronic rejection group was lower than that of healthy control group and there was no statistical difference. The ratio of CD4 + CD25 + CD127-Treg in the stable group to CD4 + T cells and health There is no statistical difference in the control group. It is suggested that the immune tolerance of the body can be effectively enhanced if the development and proliferation of the Treg are promoted.
【学位授予单位】：广西师范大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392.12;R692.5;R699
【参考文献】
相关期刊论文 前1条
1 雷树勇,谢国乾,黄承乐;血透患者细胞免疫功能的变化[J];武警医学院学报;2002年02期
，本文编号：2502610
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