大鼠延髓易化区小细胞网状核的神经纤维投射

发布时间：2019-07-01 08:37

【摘要】：小细胞网状核(PCRt)具有调节呼吸、发声、咀嚼、吞咽等口面部运动的作用;还接受中枢神经系统的投射和外周感觉信号的传入,中继本体感觉信息诱发口面部运动神经元的反射性活动。近年来对痉挛性脑瘫患儿进行选择性脊神经后根切断术后发现,患者四肢的肌张力不仅有大幅度下降,伴随的流涎症状也有不同程度的改善,提示PCRt与控制吞咽和腺体分泌的脑干内脑神经核有神经纤维联系,但目前国内对此方面的研究资料较少,本实验主要从形态学上观察该核团与直接调控吞咽和腺体分泌活动的脑神经核团间的神经联系,以及进一步了解网状结构中PCRt到脊髓颈段神经元的神经纤维投射。 方法 1.将碳素墨水微量注射注入一侧PCRt,进行HE染色,观察PCRt的定位是否准确。 2.将麦芽凝集素-辣根过氧化物酶(WGA-HRP)注入一侧PCRt,采用TMB-ST法成色,在光学显微镜下观察注射部位是否准确及标记情况。 3.将1,1′-双十八烷-3,3,3′,3′-四甲基吲哚羧花青-高氯酸盐(DiI)微量注射入一侧PCRt,取材,荧光显微镜下观察注射部位及标记情况。 结果 1.舌下神经核、迷走神经背核、面神经核、疑核、上泌涎核、下泌涎核、三叉神经脊束核、三叉神经运动核,均见有顺行标记纤维和终末,为对侧优势;孤束核、前庭内侧核见有逆行标记细胞及顺行神经纤维终末,对侧多于同侧。 2.颈段脊髓灰质,可在颈段脊髓灰质Ⅰ、Ⅱ、Ⅲ板层发现顺行标记神经纤维,在Ⅶ、Ⅷ、Ⅺ板层发现有少量逆行标记细胞及顺行标记神经纤维。 3.WGA-HRP和Dil标记的结果分布相似,不同的是,DiI在上泌涎核未显示出标记,且在其他核团标记的细胞数目及神经纤维终末没有WGA-HRP标记的多。 结论 1.通过对WGA-HRP、DiI示踪的检测,说明以小细胞网状核为主的延髓网状结构易化区和大多数脑干内脑神经核、颈段脊髓神经元之间存在直接的纤维联系。 2.从小细胞网状核到脊髓的投射纤维多经由颈段脊髓灰质的Ⅰ、Ⅱ、Ⅲ板层。而颈段脊髓灰质Ⅶ、Ⅷ、Ⅺ板层与小细胞网状核有双向神经纤维投射。
[Abstract]:Small cell reticular nucleus (PCRt) can regulate oral and facial movement such as respiration, voice, chewing, swallowing and so on. It also receives the projection of central nervous system and the afferent of peripheral sensory signal, and relays the sensory information of body to induce the reflexive activity of oral and facial motor neurons. In recent years, after selective posterior spinal nerve transection in children with spastic cerebral palsy, it was found that the muscle tension of the extremities was not only greatly decreased, but also the symptoms of salivation were improved in varying degrees, suggesting that PCRt is related to the nucleus of the brain nerve in the brain stem, which controls swallowing and gland secretion, but there is little research data in this field at present. The aim of this study was to observe the neural relationship between the nucleus and the nucleus directly regulating swallowing and gland secretion, and to further understand the nerve fiber projection from PCRt to the cervical neurons of the spinal cord in the reticular structure. Method 1. Carbon ink was injected into one side of PCRt, for HE staining to observe whether the location of PCRt was accurate. two銆，

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