大肠埃希菌Ⅰ型菌毛fimA基因的克隆和序列比较分析
发布时间：2019-07-06 09:30
【摘要】：通过设计1对fimA全基因通用引物,对已知不同人、动物源的17株大肠埃希菌菌株的Ⅰ型菌毛fimA基因进行PCR扩增、克隆和测序,并与GenBank中9株菌株序列进行同源性比较分析,探讨I型菌毛宿主嗜性。结果表明:17株菌株均能扩增出约549bp的预期大小目的片段。克隆和序列分析显示,26株不同来源的大肠埃希菌fimA基因的核苷酸同源性为88.7%~100%,推导氨基酸同源性为88%~100%,其中出现差异的31个易发生突变的位点多为中性氨基酸,其中10株菌的fimA序列于第26个氨基酸位置连续插入了脯氨酸和苏氨酸,且80%为猪源大肠埃希菌,因此提示I型菌毛在大肠埃希菌的进化过程中可能具备一定的宿主嗜性。
文内图片：
图片说明：图１不同大肠埃希菌菌株ｆｉｍＡ基因ＰＣＲ扩增结果Ｆｉｇ．１ＤｉｆｆｅｒｅｎｔＥ．ｃｏｌｉｓｔｒａｉｎｓｆｉｍＡｇｅｎｅ
[Abstract]:By designing a pair of universal primers for fimA gene, the fimA gene of type I fimbriae of 17 strains of Escherichia coli from different humans and animals was amplified, cloned and sequenced by PCR, and the homology with the sequence of 9 strains in GenBank was compared with that of 9 strains in GenBank to explore the host tropism of type I fimbriae. The results showed that the expected size and target fragments of about 549bp could be amplified by 17 strains. Cloning and sequence analysis showed that the nucleotide homology of fimA gene of 26 strains of Escherichia coli from different sources was 88.7% 鈮，
本文编号：2510924
文内图片：
图片说明：图１不同大肠埃希菌菌株ｆｉｍＡ基因ＰＣＲ扩增结果Ｆｉｇ．１ＤｉｆｆｅｒｅｎｔＥ．ｃｏｌｉｓｔｒａｉｎｓｆｉｍＡｇｅｎｅ
[Abstract]:By designing a pair of universal primers for fimA gene, the fimA gene of type I fimbriae of 17 strains of Escherichia coli from different humans and animals was amplified, cloned and sequenced by PCR, and the homology with the sequence of 9 strains in GenBank was compared with that of 9 strains in GenBank to explore the host tropism of type I fimbriae. The results showed that the expected size and target fragments of about 549bp could be amplified by 17 strains. Cloning and sequence analysis showed that the nucleotide homology of fimA gene of 26 strains of Escherichia coli from different sources was 88.7% 鈮，
本文编号：2510924
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