细粒棘球绦虫AgB5抗原表位的生物信息学预测

发布时间：2019-07-14 21:32

【摘要】：目的应用计算机在线预测软件分析细粒棘球绦虫AgB5抗原蛋白的二级结构,预测其可能形成的B细胞表位和T细胞表位,为研制安全、高效的新型包虫病基因疫苗奠定基础,也为包虫病的免疫学治疗提供新的理论依据。方法采用计算机在线预测软件SOPMA对细粒棘球绦虫AgB5抗原蛋白的二级结构进行预测和分析;采用LEPS和ABC Pred网络软件结合其表面特性、亲水性、柔韧性、可及性、可塑性等对其B细胞表位进行预测和分析;采用IEBD和SYFPEITHI在线软件对细粒棘球绦虫AgB5抗原蛋白的MHC-I类HLA-A 0201限制性T细胞表位进行预测和分析。结果采用计算机在线预测软件预测细粒棘球绦虫AgB5抗原蛋白的二级结构中α螺旋结构占76.47%,β-折叠占5.88%,无规则卷曲占17.65%。结合其表面特性、亲水性、柔韧性、可及性以及可塑性等分析得出分值较高的3个B细胞表位区域,分别为:6~14、28~33和48~52氨基酸序列,较易形成B细胞表位;通过MHC-I类HLA-A 0201限制性T细胞表位分析得出分值较高的4个T细胞表位区域,分别为:9~13、24~29、38~45和51~59氨基酸序列,较易形成T细胞表位。结论通过生物信息网络技术方法分析确定细粒棘球绦虫AgB5抗原蛋白分别存在3个B细胞抗原表位和4个T细胞抗原表位,可为细粒棘球绦虫AgB抗原性研究和高效表位疫苗研发提供参考,为临床包虫病的免疫诊断、药物治疗提供新的靶标。
[Abstract]:Objective to analyze the secondary structure of AgB5 antigen protein of Echinococcus granulosus by computer online prediction software, and to predict the B cell epitopes and T cell epitopes that may be formed, so as to lay a foundation for the development of a safe and efficient new type of echinococcosis gene vaccine, and to provide a new theoretical basis for immunological treatment of echinococcosis. Methods the secondary structure of AgB5 antigen protein of Echinococcus granulosus was predicted and analyzed by computer online prediction software SOPMA, and the B cell epitopes of Echinococcus granulosus were predicted and analyzed by LEPS and ABC Pred network software combined with its surface characteristics, hydrophilicity, flexibility, accessibility and plasticity. The MHC-I class HLA-A 0201 restricted T cell epitopes of Echinococcus granulosus AgB5 antigen protein were predicted and analyzed by IEBD and SYFPEITHI online software. Results the secondary structure of AgB5 antigen protein of Echinococcus granulosus was predicted by computer online prediction software. The secondary structure of Echinococcus granulosus AgB5 antigen protein was 76.47%, 尾-fold 5.88% and irregular curl 17.65%. According to the analysis of its surface characteristics, hydrophilicity, flexibility, accessibility and plasticity, three B cell epitope regions with high score were obtained, which were 6: 14, 28 / 33 and 48 脳 52 amino acid sequences, which were easy to form B cell epitopes, and four T cell epitopes with higher scores were obtained by MHC-I HLA-A 0201 restricted T cell epitope analysis, which were 9: 13, 24 / 29, 38 / 45 and 51 / 59 amino acid sequences, which were easy to form T cell epitopes. Conclusion it is confirmed that there are three B cell epitopes and four T cell epitopes in AgB5 antigen protein of Echinococcus granulosus by bioinformation network technique, which can provide reference for the study of antigenicity of Echinococcus granulosus AgB and the development of high efficiency epitope vaccine, and provide a new target for clinical immune diagnosis and drug treatment of echinococcosis.
【作者单位】：新疆医科大学第一附属医院新疆重大疾病医学重点实验室-省部共建国家重点实验室培育基地;新疆医科大学基础医学院;
【基金】：国家自然科学基金项目(No.81260253,31160194,30960358,81160200,81060135) 新疆维吾尔自治区自然基金项目(No.2012211A304)
【分类号】：R383.33

【相似文献】