不同剂量脂多糖对缺血再灌注大鼠心肌功能损害的实验研究

发布时间：2018-03-07 11:21

本文选题：脂多糖LPS　切入点：心肌缺血再灌注　出处：《临床心血管病杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:探讨脂多糖(LPS)对缺血再灌注大鼠心肌功能的影响。方法:应用不同剂量的LPS腹腔注射建立大鼠败血症模型,通过对大鼠心脏左前降支阻塞40min建立心肌缺血再灌注损伤模型。STNFaR注射阻断LPS对心肌的效应。结果:心肌收缩功能指标dp/dt在缺血期从(4 831±213)mmHg/s降至(2 265±114)mmHg/s,心肌舒张功能指标-dp/dt由(4 119±179)mmHg/s降至(2 056±109)mmHg/s。低剂量LPS(1μg)对缺血心肌的收缩功能dp/dt和舒张功能-dp/dt没有影响,高浓度的LPS能够抑制心肌收缩和舒张功能。心肌缺血后血清肌钙蛋白I浓度升至(9.6±2.5)ng/dl,伴随着LPS注射浓度增加而升高。阻断肿瘤坏死因子-α(TNF-α)效应后并不能改善缺血心肌收缩和舒张功能,但能减轻LPS所致心肌细胞损伤。结论:低剂量的LPS对缺血再灌注大鼠心肌功能没有明显影响,而高剂量LPS导致心功能功能障碍和心肌损伤加重。
[Abstract]:Objective: to investigate the effect of lipopolysaccharide (LPS) on myocardial function in rats with ischemia reperfusion. Methods: the septicemia model of rats was established by intraperitoneal injection of different doses of LPS. The myocardial ischemia-reperfusion injury model was established by occlusion of left anterior descending branch in rats. STNFaR was injected to block the effect of LPS on myocardium. Results: myocardial systolic function index dp/dt decreased from 4,831 卤213mm Hg / s to 2.265 卤114mm Hg / s during ischemic period, and myocardial diastolic function index decreased to 2.265 卤114mm Hg / s, and myocardial diastolic function index decreased from 4.831 卤213mm Hg / s to 2.265 卤114mm Hg / s, respectively. Standard DP / DT decreased from 4 119 卤179 mm Hg / s to 2 056 卤109 mm Hg / s. Low dose LPS(1 渭 g had no effect on systolic function dp/dt and diastolic function of ischemic myocardium -dp / DT. High concentration of LPS can inhibit myocardial contraction and diastolic function. After myocardial ischemia, serum troponin I concentration rises to 9.6 卤2.5 ng / dl, which increases with the increase of LPS injection concentration. Blocking the effect of tumor necrosis factor- 伪 (TNF- 伪) does not improve the ischemic heart. Muscle contraction and diastolic function, Conclusion: low dose of LPS has no obvious effect on myocardial function of ischemia-reperfusion rats, while high dose of LPS leads to dysfunction of cardiac function and exacerbation of myocardial injury.
【作者单位】：山西医科大学第一医院心胸外科;美国辛辛那提大学;山西医科大学第一医院心内科;
【基金】：山西省科技发展计划(社会发展)项目(No:20130313016-12)
【分类号】：R515.3;R54

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