硫化氢通过下调MMP-11和MMP-16改善高甲状腺素诱导的大鼠心肌纤维化

发布时间：2018-05-06 01:07

本文选题：甲状腺功能亢进 + 心肌纤维化　；参考：《中国老年学杂志》2017年20期

【摘要】：目的观察硫化氢(H2S)对甲状腺激素诱导的甲状腺功能亢进大鼠心肌纤维化的影响。方法通过左旋甲状腺素溶液(L-Thy,250μg/ml)腹腔注射(10 ml·kg-1·d-1)4 w,建立甲状腺功能亢进大鼠模型。以硫氢化钠(Na HS)作为外源性H2S供体,随机将40只SD雄性大鼠等分为对照组(Control组)、模型组(L-Thy组)、H2S干预组(L-Thy+H2S组)和H2S对照组(H2S组),4 w后处死大鼠,取左室心肌组织行Masson染色观察心肌纤维化,测量胶原容积分数(CVF),Western印迹检测MMP-11、MMP-16、PKCα靶蛋白的表达。结果与Control组相比,L-Thy组心肌胶原纤维堆积明显增加,CVF增高(P0.05),而与L-Thy组相比,L-Thy+H2S组纤维化得到明显改善,CVF降低(P0.05);H2S组与Control组镜下改变类似,CVF无明显差异(P0.05);与Control组相比,L-Thy组MMP-11、MMP-16表达有显著差异(P0.05);与L-Thy组相比,L-Thy+H2S组MMP-11、MMP-16表达明显减低(P0.05),H2S组与Control组上述蛋白表达水平无明显差异(P0.05)。各组之间PKCα蛋白表达没有明显差异(P0.05)。结论 H2S可改善甲状腺激素诱导的甲状腺功能亢进大鼠心肌纤维化,其机制可能与下调MMP-11、MMP-16表达有关,但与PKCα通路无关。
[Abstract]:Aim to observe the effect of H 2 S on myocardial fibrosis induced by thyroid hormone in hyperthyroidism rats. Methods the model of hyperthyroidism was established in rats with hyperthyroidism by intraperitoneal injection of 10 ml kg-1 d ~ (-1) for 4 weeks. 40 male SD rats were randomly divided into control group, model group, L-Thy H2S group and H2S control group. Myocardial fibrosis was observed by Masson staining in left ventricular myocardium, and the expression of MMP-11, MMP-16 and PKC 伪 target protein was detected by measuring collagen volume fraction (CVF) and Western blotting. Results compared with Control group, myocardial collagenous fiber accumulation in L-Thy group was significantly increased (P 0.05), while fibrosis in L-Thy H2S group was significantly improved compared with L-Thy group. There was no significant difference between Control group and L-Thy H2S group (P 0.05). There was a significant difference in the expression of MMP-11 and MMP-16 between the two groups (P 0.05), and the expression of MMP-11 MMP 16 in the L-Thy H2S group was significantly lower than that in the L-Thy group. There was no significant difference between the two groups in the expression of MMP-11 and MMP-16 in the P0.05H _ 2S group and the Control group. There was no significant difference in the expression of PKC 伪 protein among the three groups (P 0.05). Conclusion H2S can improve the myocardial fibrosis induced by thyroid hormone in hyperthyroidism rats. The mechanism may be related to the down-regulation of MMP-11 MMP-16 expression, but not to the PKC 伪 pathway.
【作者单位】：南华大学附属第一医院心内科;南华大学附属第二医院药剂科;
【基金】：国家自然科学基金(81270181;81202830)
【分类号】：R54

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