骨桥蛋白的增龄性变化及其与冠心病的关系

发布时间：2018-07-17 05:26

【摘要】：目的探讨骨桥蛋白(OPN)的增龄性变化及其与冠心病的关系。方法收集健康青年(40岁以下)、中年(40~65岁)、老年组(65岁),并经冠脉造影结果诊断无冠心病的血清标本各20份,并收集冠心病非老年组(65岁)及冠心病老年组(65岁)标本各20例,所有冠心病患者诊断均以冠脉造影结果为准,并进行Western印迹法检测OPN的含量,并同时采集各组吸烟史,饮酒史,血脂等生化指标。结果 OPN在老年组中呈阳性表达,而在青、中年组呈阴性;老年组、中年组及青年组OPN光密度值分别为7.34×10~4±8.97×10~3,1.34×10~3±4.15×10~2,1.58×10~2±0.096×10~2,各组间差异显著(F=4.29,P=0.02);OPN在冠心病非老年组和老年组间表达无统计学差异(P0.05)。结论 OPN存在增龄性变化,当存在冠脉病变时,OPN不再有年龄差异,可能对冠心病的发生存在作用。
[Abstract]:Objective to investigate the aging changes of osteopontin (OPN) and its relationship with coronary heart disease (CHD). Methods 20 healthy young people (under 40 years old), middle age (40 to 65 years old), old age group (65 years old), and 20 serum samples each with no coronary heart disease diagnosed by coronary angiography were collected, and 20 samples were collected from non-elderly patients with coronary heart disease (65 years old) and 20 samples from elderly patients with coronary heart disease (65 years old). All the patients with coronary heart disease were diagnosed by coronary angiography, and the content of OPN was detected by Western blotting, and the biochemical indexes such as smoking history, drinking history, blood lipid and so on were collected at the same time. Results the optical density of OPN in the elderly group was 7.34 脳 10 ~ (4) 卤8.97 脳 10 ~ (3) 卤1.34 卤4.15 脳 10 ~ (2) 卤1.58 脳 10 ~ (2) 2 卤0.096 脳 10 ~ (2). There was no significant difference between the two groups (P 0.05). Conclusion there are age-related changes in OPN. When there is coronary artery disease, there is no age difference in OPN, which may play an important role in the occurrence of coronary heart disease.
【作者单位】： 湖北医药学院附属太和医院;
【基金】：湖北省教育厅科学研究计划资助项目(D20152101)
【分类号】：R541.4
，

本文编号：2129211