IGF-1的抗动脉粥样硬化机制研究
发布时间：2018-08-07 11:54
【摘要】：目的:观察IGF-1对SD大鼠主动脉弓动脉粥样硬化病变处的巨噬细胞、TNF-α和血管平滑肌细胞的影响,以探讨其可能的抗动脉粥样硬化机制,为其临床应用提供理论依据和实践指导。方法:共24只8周龄大小的SD大鼠,随机分为模型组(12只)和IGF-1组(12只),分别测量它们的体重(单位为g)、血压(单位为mmHg)及血脂(单位为mmol/L)水平作为基础值。IGF-1组给予高脂饮食+腹腔注射IGF-1,所用剂量为1ml(0.1mg/ml),模型组给予高脂饮食+腹腔注射生理盐水1.0ml,于高脂饲料喂养16周后再次测量剩余所有SD大鼠的体重、血压及血脂水平,获取最终剩余的两组SD大鼠的主动脉弓进行病理学分析:HE染色观察主动脉弓横断面的动脉粥样硬化病变程度;免疫组化法半定量分别分析主动脉弓横断面的动脉粥样硬化病变处TNF-α、巨噬细胞标志物CD68及血管平滑肌细胞标志物α-SMA各自的光密度值(Optical density,OD)。结果:与模型组相比,IGF-1组SD大鼠的体重、血压及血脂无显著差异(P0.05),巨噬细胞标志物CD68及TNF-α各自的OD值水平显著减少(P0.05),同时血管平滑肌细胞标志物α-SMA的OD值显著增加(P0.05)。结论:IGF-1可能通过减少动脉粥样硬化斑块内炎症及炎症因子水平、增加血管平滑肌细胞数量来发挥抗动脉粥样硬化作用。
[Abstract]:Objective: to observe the effect of IGF-1 on macrophage TNF- 伪 and vascular smooth muscle cells (VSMC) in atherosclerotic lesions of aortic arch of SD rats, and to explore its possible anti-atherosclerosis mechanism and to provide theoretical basis and practical guidance for its clinical application. Methods: a total of 24 8-week-old SD rats were used. They were randomly divided into model group (12 rats) and IGF-1 group (12 rats). Their body weight (g), blood pressure (mmHg) and blood lipid (mmol/L) level were measured as basic values respectively. IGF-1 group was given high fat diet intraperitoneal injection of IGF-1, the dose was used. For 1ml (0.1mg/ml), the model group was given a high-fat diet intraperitoneal injection of physiological saline 1.0 ml. After 16 weeks of high-fat diet feeding, the body weight of all the remaining SD rats was measured again. Blood pressure and blood lipid levels were obtained from the aortic arch of the remaining two groups of SD rats. The pathological analysis was performed to observe the degree of atherosclerosis on the cross section of the aortic arch. The optical density (OD) of TNF- 伪, macrophage marker CD68 and vascular smooth muscle cell marker 伪 -SMA in atherosclerotic lesions of the aortic arch were analyzed by immunohistochemistry. Results: compared with the model group, the weight, blood pressure and blood lipid of SD rats in IGF-1 group showed no significant difference (P0.05). The OD levels of macrophage markers CD68 and TNF- 伪 were significantly decreased (P0.05), while the OD value of vascular smooth muscle cell marker 伪 -SMA was significantly increased (P0.05). ConclusionTwo one IGF-1 may play an anti-atherosclerotic effect by reducing the levels of inflammation and inflammatory factors in atherosclerotic plaques and increasing the number of vascular smooth muscle cells.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R543.5
本文编号：2169942
[Abstract]:Objective: to observe the effect of IGF-1 on macrophage TNF- 伪 and vascular smooth muscle cells (VSMC) in atherosclerotic lesions of aortic arch of SD rats, and to explore its possible anti-atherosclerosis mechanism and to provide theoretical basis and practical guidance for its clinical application. Methods: a total of 24 8-week-old SD rats were used. They were randomly divided into model group (12 rats) and IGF-1 group (12 rats). Their body weight (g), blood pressure (mmHg) and blood lipid (mmol/L) level were measured as basic values respectively. IGF-1 group was given high fat diet intraperitoneal injection of IGF-1, the dose was used. For 1ml (0.1mg/ml), the model group was given a high-fat diet intraperitoneal injection of physiological saline 1.0 ml. After 16 weeks of high-fat diet feeding, the body weight of all the remaining SD rats was measured again. Blood pressure and blood lipid levels were obtained from the aortic arch of the remaining two groups of SD rats. The pathological analysis was performed to observe the degree of atherosclerosis on the cross section of the aortic arch. The optical density (OD) of TNF- 伪, macrophage marker CD68 and vascular smooth muscle cell marker 伪 -SMA in atherosclerotic lesions of the aortic arch were analyzed by immunohistochemistry. Results: compared with the model group, the weight, blood pressure and blood lipid of SD rats in IGF-1 group showed no significant difference (P0.05). The OD levels of macrophage markers CD68 and TNF- 伪 were significantly decreased (P0.05), while the OD value of vascular smooth muscle cell marker 伪 -SMA was significantly increased (P0.05). ConclusionTwo one IGF-1 may play an anti-atherosclerotic effect by reducing the levels of inflammation and inflammatory factors in atherosclerotic plaques and increasing the number of vascular smooth muscle cells.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R543.5
【参考文献】
相关期刊论文 前6条
1 郜娜;杨庆宇;刘秀梅;;丁苯酞对ApoE~(-/-)小鼠动脉粥样硬化及主动脉壁VCAM-1表达的影响[J];中国病理生理杂志;2016年06期
2 曾翠玲;郑凌云;李香莉;王丽京;;血管平滑肌细胞表型转化影响因素及中药干预研究进展[J];广东药学院学报;2016年02期
3 李靓;谢巍;姜志胜;尹卫东;唐朝克;;我国动脉粥样硬化基础研究近三年进展[J];中国动脉硬化杂志;2015年11期
4 周红;吴晓燕;袁艺标;戚晓红;;三种剂量维生素D_3结合高脂饲料建立大鼠动脉粥样硬化模型的比较[J];中国动脉硬化杂志;2012年11期
5 杨宏辉;高传玉;徐予;陈岩;张嘉莹;;动脉粥样硬化SD大鼠模型的建立[J];医药论坛杂志;2009年02期
6 孙安阳,俞彰,钟慈声,王永铭,杨藻宸;大鼠动脉钙超负荷模型的建立及确证[J];中华医学杂志;1999年10期
，本文编号：2169942
本文链接：https://www.wllwen.com/yixuelunwen/xxg/2169942.html
最近更新
教材专著
