核仁素对糖尿病性心肌病小鼠心肌细胞凋亡的影响

发布时间：2018-08-07 13:09

【摘要】：目的:探讨核仁素对糖尿病性小鼠心肌病心肌细胞凋亡的影响。方法:小鼠给予高脂高糖饲料喂养,第5,6周分别予链脲佐菌素60 mg/kg腹腔注射,构建2型糖尿病模型;实验分为4组:野生型小鼠对照组、核仁素转基因小鼠对照组、野生型小鼠糖尿病组、核仁素转基因小鼠糖尿病组;第8周末检测糖尿病相关指标;第20周末,采用HE染色观察心肌形态学改变;TUNEL染色和caspase-3活性检测心肌细胞凋亡程度。结果:糖尿病组小鼠空腹血糖值较野生型对照组明显升高;与野生型对照组相比,野生型糖尿病组小鼠心肌HE染色显示细胞排列紊乱,部分肌纤维出现断裂及溶解,TUNEL染色和caspase-3活性检测显示心肌细胞凋亡明显增加。与野生型糖尿病组小鼠相比,转基因糖尿病组小鼠心肌形态学改变明显减轻,心肌细胞凋亡明显减少。结论:核仁素过表达可抑制糖尿病性心肌病中心肌细胞凋亡的发生。
[Abstract]:Objective: To investigate the effect of nucleolus on cardiomyocyte apoptosis in diabetic cardiomyopathy. Methods: mice were fed with high fat and high glucose diet and injected with streptozotocin 60 mg/kg at week 5,6 to construct type 2 diabetes model, and the experiment was divided into 4 groups: wild type mice control group, nucleolus transgenic mice control group and wild type mice sugar. Urine disease group, nucleolus transgenic mice diabetes group, eighth weekend detection of diabetes related indicators, Twentieth weekend, the use of HE staining to observe the myocardial morphological changes, TUNEL staining and caspase-3 activity to detect the degree of cardiomyocyte apoptosis. Results: diabetic mice fasting blood glucose level is significantly higher than the wild type control group, and the wild type control group. HE staining of myocardium in the wild type diabetic mice showed that the cells were arranged in disorder and some muscle fibers were broken and dissolved. TUNEL staining and caspase-3 activity detected that the apoptosis of cardiac myocytes increased significantly. Compared with the wild type diabetic mice, the morphological changes of cardiac muscle in the transgenic diabetic mice were obviously reduced, and the apoptosis of cardiac myocytes was obvious. Conclusion: nucleolus overexpression can inhibit the occurrence of cardiomyocyte apoptosis in diabetic cardiomyopathy.
【作者单位】：中南大学湘雅医学院病理生理学系;
【基金】：国家自然科学基金(81170113)~~
【分类号】：R587.2;R542.2

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