辛伐他汀通过上调动脉粥样硬化大鼠血管壁Bcl-2蛋白的表达而抑制细胞凋亡

发布时间：2018-10-05 15:58

【摘要】：目的探讨辛伐他汀对动脉粥样硬化(AS)大鼠模型血管壁细胞凋亡、Bcl-2蛋白的表达干预作用。方法高脂饲料及腹腔注射维生素D_3复制大鼠AS模型,随机分为对照组(n=10)给予普通饲料喂养,实验组(n=13)给予高脂饲料喂养,干预组(n=13)给予高脂饲料喂养基础上加用辛伐他汀干预,11周后取胸主动脉,观察其斑块变化。采用免疫组化Elivision法测定AS血管壁中Bcl-2蛋白表达。通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TUNEL)法检测细胞凋亡指数(AI),分析各组中Bcl-2表达及AI变化。结果与对照组比较,Bcl-2蛋白的表达在实验组明显降低(P0.05);而与实验组比较,干预组Bcl-2蛋白的表达明显升高(P0.05),且与对照组比较无差异。同时,AI在各组中的变化比较则相反,差异均有统计学意义(P0.05)。结论辛伐他汀抗大鼠AS的作用可能与上调Bcl-2蛋白表达,进而抑制细胞凋亡相关。
[Abstract]:Objective to investigate the effect of simvastatin on the expression of Bcl-2 protein in vascular parietal cells of atherosclerotic (AS) rats. Methods the AS model of rats was induced by high fat diet and intraperitoneal injection of vitamin D 3. The rats were randomly divided into two groups: control group (n = 10) and experimental group (n = 13) were fed with high fat diet. The thoracic aorta was taken after 11 weeks of intervention with simvastatin on the basis of high fat diet in the intervention group (nong13), and the plaque changes were observed. The expression of Bcl-2 protein in vascular wall of AS was determined by immunohistochemical Elivision method. Terminal deoxynucleotidyl transferase-mediated dUTP Nick end labeling (TUNEL) was used to detect the expression of Bcl-2 and the change of AI in all groups by (AI),. Results compared with the control group, the expression of Bcl-2 protein was significantly decreased in the experimental group (P0.05), but the expression of Bcl-2 protein in the intervention group was significantly higher than that in the control group (P0.05), and there was no difference between the intervention group and the control group. At the same time, the changes of AI in each group were opposite, the differences were statistically significant (P0.05). Conclusion the effect of simvastatin on rat AS may be related to up-regulation of Bcl-2 protein expression and inhibition of apoptosis.
【作者单位】：蚌埠医学院第一附属医院心血管科;
【基金】：安徽高校研究重点项目(SK2016A0595) 蚌埠市科技局课题(20150336) 蚌埠医学院科研课题资助(BY0855)
【分类号】：R543.5

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