依那普利叶酸片对H型高血压大鼠外周血炎症因子及颈动脉结构的影响

发布时间：2019-07-14 17:45

【摘要】：目的研究依那普利叶酸对H型高血压大鼠外周血炎症因子水平及颈动脉结构的影响。方法按照体重将自发性高血压雄性大鼠随机分4组:对照组、模型组、依那普利组以及依叶组,每组各15只。除对照组外,各组均给予含3%蛋氨酸的饲料喂养建立H型高血压大鼠模型。模型建立后,依叶组给予10m L·kg~(-1)马来酸依那普利叶酸溶液灌胃;依那普利组给予10 m L·kg~(-1)马来酸依那普利溶液灌胃;模型组和对照组分别给予10 m L·kg~(-1)双蒸水灌胃,均每日1次,连续给药8周。用酶联免疫法测定血清同型半胱氨酸(Hcy)、血清肿瘤坏死因子α(TNF-α)、超敏C反应蛋白(hs-CRP)及可溶性OX40配体(OX40L)水平,终点比色法检测一氧化氮(NO)水平,显微镜下观察颈动脉的中膜厚度(IMT)、颈动脉内径(LD)以及颈动脉中膜横截面积(IMCSA)。结果与依那普利组的血清Hcy与NO水平分别为(64.22±6.82),(41.45±5.25)μmol·L~(-1)相比,依叶组的血清Hcy与NO水平分别为(19.75±2.14),(57.68±6.43)μmol·L~(-1),差异均有统计学意义(均P0.05)。与依那普利组的TNF-α、hs-CRP与OX40L分别为(72.72±6.89),(1.15±0.12)pg·m L~(-1)与(5.24±0.47)ng·m L~(-1)相比,依叶组的TNF-α、hs-CRP与OX40L分别为(58.18±5.76),(0.86±0.11)pg·m L~(-1)与(3.03±0.39)ng·m L~(-1),差异均有统计学意义(均P0.05)。与依那普利组的颈动脉IMT(61.14±7.73)μm、IMCSA水平(0.12±0.02)mm2比较,依叶组大鼠颈动脉IMT(52.74±6.29)μm、IMCSA(0.10±0.01)mm2水平,差异均有统计学意义(均P0.05)。与依那普利组大鼠颈动脉LD水平(951.38±6.08)μm比较,依叶组大鼠颈动脉LD水平(969.79±6.17)μm,差异有统计学意义(P0.05)。结论依那普利叶酸能够明显降低H型高血压大鼠外周血Hcy及TNF-α、hs-CRP及OX40L水平,提高NO水平,减轻炎症反应,改善颈动脉结构。
[Abstract]:Objective to study the effect of enalapril folic acid on peripheral blood inflammatory factor level and carotid artery structure in H-type hypertensive rats. Methods male spontaneously hypertensive rats were randomly divided into 4 groups according to body weight: control group, model group, enalapril group and enalopril group with 15 rats in each group. Except the control group, the H-type hypertensive rat model was established by feeding with 3% methionine. After the establishment of the model, the Enalapril Maleate folic acid solution was given intragastrically in the enalapril group, 10ml kg~ (- 1) enalapril Maleate solution was given intragastrically in the enalapril group, and 10ml kg~ (- 1) double steamed water was given intragastrically once a day for 8 weeks in the model group and the control group, respectively. Serum tumor necrosis factor 伪 (TNF- 伪), high sensitive C reactive protein (hs-CRP) and soluble OX40 ligand (OX40L) were measured by enzyme linked immunosorbent assay (Elisa). Nitric oxide (NO) (no (NO) was measured by end point colorimetric assay. Carotid artery thickness (IMT), carotid artery diameter (LD) and carotid media cross section area (IMCSA). Were observed under microscope. Results compared with enalapril group, serum Hcy and NO levels were (64.22 卤6.82), (41.45 卤5.25) 渭 mol 路L ~ (- 1), and serum Hcy and NO levels were (19.75 卤2.14), (57.68 卤6.43) 渭 mol 路L ~ (- 1), respectively, and the difference was statistically significant (P 0.05). Compared with enalapril group, TNF- 伪, hs-CRP and OX40L were (72.72 卤6.89), (1.15 卤0.12) pg 路mL ~ (- 1) and (5.24 卤0.47) ng 路mL ~ (- 1), respectively, and TNF- 伪, hs-CRP and OX40L were (58.18 卤5.76), (0.86 卤0.11) pg 路mL ~ (- 1) and (3.03 卤0.39) ng 路mL ~ (- 1), respectively. The difference was statistically significant (P 0.05). Compared with the carotid artery IMT (61.14 卤7.73) 渭 m and (0.12 卤0.002) mm2 in the enalapril group, the IMT and mm2 levels in the carotid artery of the enalapril group were (52.74 卤6.29) 渭 m and (0.10 卤0.001) 渭 m, respectively (P 0.05). Compared with the level of LD in carotid artery of enalapril group (951.38 卤6.08) 渭 m, the level of LD in carotid artery of enalapril group was (969.79 卤6.17) 渭 m, the difference was statistically significant (P 0.05). Conclusion Enalapril folic acid can significantly decrease the levels of Hcy, TNF- 伪, hs-CRP and OX40L in peripheral blood of H type hypertensive rats, increase the level of NO, reduce the inflammatory reaction and improve the structure of carotid artery.
【作者单位】：长沙市第一医院神经内科;湘潭市中心医院;北京大学第一医院;
【基金】：国家十二五重大新药创制科技重大专项基金资助项目(2012ZX09101105)
【分类号】：R544.1

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