水仙‘金玉’花芽分化研究

发布时间：2018-01-05 04:22

本文关键词：水仙‘金玉’花芽分化研究　出处：《福建农林大学》2017年硕士论文　论文类型：学位论文

【摘要】：本研究以'金玉'水仙为试验材料,研究'金玉'在普通贮藏环境条件下花芽的发育分化过程,通过石蜡切片技术结合对花芽分化期内激素的测定,了解了'金玉'花芽的形态分化过程以及其内源激素的相应变化,以期为'金玉'花芽分化的调控、花期调控、栽培管理等生产实践提供相应的理论基础和技术参考。其主要研究结果如下:1.对三年生'金玉'和'金盏银台' 2个品种的主鳞茎球花芽分化的过程进行了对照观察比较,结果表明:在试验设计的时间范围内,'金玉'的花芽分化经历了 6个时期,分别是:叶芽时期、花序原基形成期、佛焰状总苞形成期、花原基形成期、花冠形成期,雄蕊形成期。其中佛焰总苞形成期到花原基形成期历时较长,持续了 25 d左右。进入9月份,花器官分化速度明显快于前期。本次试验中'金盏银台'分化完成时间约95 d,'金玉'在花芽分化速度上明显滞后,比'金盏银台'发育晚2个时期。另外本次实验结果中与张晓晴观察'金盏银台'的试验结果不同,副花冠是在雌蕊生长后才开始生长,所以与钟衡观察到的结果相同,应分为8个时期。2.在'金玉'花芽分化过程中,对IAA、ABA、GA3、ZR、ZA进行了检测,结果显示:在整个花芽分化期,IAA含量随着器官的发育状况变化较大,因而I AA可能存在促进和抑制花芽分化的两种不同作用;在花序形成期和花冠形成期这两个时期ABA含量被持续消耗,总体趋势呈"M"走势,即先上升后下降的重复变化,而这两个时期是'金玉'花芽分化速度最慢的时期。这两个时期分化进程缓慢直接导致滞后'金盏银台'2个形成期的时间。因此推测ABA很可能对'金玉'花芽的发育有抑制作用;在对'金玉'和'金盏银台'内源激素测定时发现,'金玉'内源GA3的含量是'金盏银台'的两倍;在花芽发育初期ZA和ZR有个积累的过程,'金玉'在ZA和ZR从7月5日到8月9日处于积累阶段,长达35 d之后ZA和ZR才快速的被消耗,而'金盏银台'积累时间为'金玉'的1/3,呈现出快速积累—快速消耗—快速积累—快速消耗的过程,结合石蜡切片与内源激素的变化规律可以发现与两者发育动态相吻合,由此说明在花芽发育的前期,较高浓度的ZR促使细胞分裂的发育速度加快,对开花诱导起着明显的促进作用。在'金玉'和'金盏银台'花芽分化的不同阶段,不仅各种内源激素水平发生显著变化,其内源激素比例也显著改变。但相同的是高的IAA/GA有利于花芽形态的分化,不利于花芽原基的发育。高水平的ABA/GA既有利于花芽原基的发育又有利于花芽形态的分化。
[Abstract]:In this study, 'Jinyu' as test material development and differentiation of Narcissus, study the process of 'Jinyu' in the ordinary storage environment under the condition of flower bud, through paraffin section technique combined with the measurement of the flower bud differentiation period of hormone, understand the morphological differentiation of Flower Bud 'Jinyu' and the corresponding changes of endogenous hormones, in order to 'fair' the regulation of flower bud differentiation, flowering regulation, and provide a theoretical basis and technical reference for the corresponding cultivation management practice. The main research results are as follows: 1. the main bulb of flower bud differentiation of three years' fair 'and' jinzhanyintai '2 varieties of the process were observed and compared, results show that: in the experiment period,' flower bud differentiation 'Jinyu experienced 6 periods, namely: bud period, inflorescence primordium, Buddha flame shaped involucre formation, Hua Yuanji formation period, corolla formation period, stamen formation period in which the Buddha. Flame involucre formation stage to flower primordia formation period lasted longer and lasted about 25 D. In September, flower organ differentiation significantly faster than the previous. In this test 'jinzhanyintai' differentiation time of about 95 D, 'Jinyu' obviously lags in the bud differentiation, developed later than 'jinzhanyintai' 2 times. In addition, the results of the experiment and observation of Zhang Xiaoqing 'jinzhanyintai' different corona is in growth after pistil begin to grow, so the clock balance observed the same, should be divided into 8 periods in the '.2.' Jinyu during flower bud differentiation of IAA ABA, GA3, ZR, ZA were detected, the results showed that: in the flower bud differentiation period, the content of IAA changes with the development of organ is large, so the I AA may have two different effects of promotion and inhibition of flower bud differentiation; inflorescence formation stage and corolla formation ABA content of the two period was continued Consumption, the overall trend showed "M" trend, namely the repetition of the first increased and then decreased, while the two period is the 'speed of flower bud differentiation of Jinyu' the slowest period. The two period of the slow process of differentiation directly lead lag 'jinzhanyintai'2 formation period. So we speculate that ABA is likely to' Jinyu 'flower buds have inhibitory effect on the determination; found in' Jinyu 'and' jinzhanyintai 'endogenous hormone content,' Jinyu 'endogenous GA3 is two times the' jinzhanyintai '; in the early stage of ZA and ZR is a process of accumulation of flower bud development,' Jin 'in ZA and ZR from from July 5th to August 9th in the accumulation stage, up to 35 d after ZA and ZR was quickly consumed, and' jinzhanyintai 'accumulation time for' 1/3 '", showing the process of rapid accumulation and rapid consumption of rapid accumulation of fast consumption, combined with the variation of paraffin and endogenous hormones can be found with the two. Dynamic development is consistent, so that in the early stage of flower bud development, development speed of high concentrations of ZR promote cell division to accelerate, flowering induction plays a significant role in promoting. In different stages of 'Jinyu' and 'jinzhanyintai' flower bud differentiation, endogenous hormone levels not only all have significant changes of the endogenous hormone the proportion of significant change. But the same is high IAA/GA is favorable to the differentiation of flower bud, not conducive to the development of the floral primordium. High level of ABA/GA is conducive to the development and differentiation of floral primordium to flower bud.

【学位授予单位】：福建农林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S682.21

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