亚麻籽对延边黄牛体组织脂代谢关键酶、调节因子及相关调控激素基因表达的影响

发布时间：2018-01-27 13:31

本文关键词： 延边黄牛亚麻籽脂肪组织调节因子酶调控激素　出处：《延边大学》2017年硕士论文　论文类型：学位论文

【摘要】：日粮中的多不饱和脂肪酸具有多种作用,参与反刍动物体内脂肪酸的合成代谢,并参与调控多种与脂代谢相关的酶和基因的表达。本试验旨在研究亚麻籽对延边黄牛背最长肌与皮下脂肪组织中与脂代谢相关的调节因子、关键酶、相关调控激素基因表达的影响。本试验选用30头体况相近、健康无病、体重为(480±15)kg的延边黄牛阉牛,采取单因子随机分为3组(n=10),CON组(对照组)、WL组和CL组牛饲喂的育肥期基础日粮中分别添加0%亚麻籽、8%整粒亚麻籽、8%破碎亚麻籽。利用气相色谱仪检测延边黄牛背最长肌与皮下脂肪组织中长链脂肪酸的含量,利用实时荧光定量PCR技术检测背最长肌、皮下脂肪组织中与脂代谢相关的调节因子(SREBPlc,PPARγ,LXRα)、关键酶(SCD,ACC,FAS,LPL,C/EBPα,C/EBPβ,CPT1β)、相关调控激素(GHR,InsR,GLNR,TNFa,Interleukin-6,Adiponectin)的基因表达,采用蛋白免疫印迹法检测皮下脂肪组织中与脂代谢相关调节因子(SREBP1c,PPARy,LXRα)蛋白表达情况。试验结果表明:(1)日粮添加8%亚麻籽,能够提高延边黄牛脂肪组织和肌肉组织中n-3 PUFAs、n-6 PUFAs的含量,降低饱和脂肪酸(棕榈酸、硬脂酸)的含量;(2)脂肪组织中n3PUFAs、n6PUFAs含量的提高,能够促进延边黄牛皮下脂肪组织中参与脂代谢的转录因子SREBP-1C、PPAR,y的基因表达和蛋白表达水平,且亚麻籽经破碎处理后影响更明显,脂肪组织中LXRα基因表达水平和蛋白表达也与亚麻籽破碎处理有关;(3)提高饲粮中n-3 PUFAs、n-6 PUFAs的含量有利于提高脂肪组织中脂肪酸合成关键酶类SCD、FAS、ACC、LPL的表达水平升高,对C/EBPα、C/EBPβ的影响效果低于关键酶类,n-3 PUFAs、n-6 PUFAs对脂肪组织中CPT1的提高高于肌肉组织;(4)亚麻籽的添加有利于提高脂肪组织中GHR、GLNR和细胞因子(Adiponectin、IL-6、TNFα)的基因表达水平,且脂肪组织中促进效果最明显,Ins R的表达与GLNR的表达趋势相反。综上所述:(1)延边黄牛日粮中添加8%的亚麻籽有利于提高背最长肌和皮下脂肪组织中C18:1、C18:2、C18:2n-6、C18:3n-3的含量,降低C16:0、C18:0的含量,经破碎处理的亚麻籽效果更好。(2)脂肪组织中n-3 PUFAs、n-6 PUFAs的提高,上调了延边黄牛皮下脂肪组织中转录因子SREBP-1c、PPARy基因和蛋白表达水平,经破碎处理的亚麻籽影响更明显。(3)提高饲粮中亚麻籽的含量有利于提高脂肪组织中脂肪酸合成相关酶类(SCD、FAS、ACC、LPL)、相关调控激素(GHR、GLNR、TNFα、ADI、IL-6)基因表达水平。
[Abstract]:Polyunsaturated fatty acids (PUFA) in diets have many effects and participate in the synthesis and metabolism of fatty acids in ruminants. The aim of this study was to study the regulation factors and key enzymes related to lipid metabolism in longissimus dorsi muscle and subcutaneous adipose tissue of Yanbian Yellow cattle. The effect of related regulation hormone gene expression. In this experiment, 30 cattle with similar body condition, healthy and no disease, weight of 480 卤15 kg Yanbian cattle castrated cattle were selected. A single factor was randomly divided into 3 groups (control group WL group and CL group) fed with 0% flaxseed and 8% whole flax seed respectively. 8% broken flax seeds. The content of long chain fatty acids in longissimus dorsi and subcutaneous adipose tissue of Yanbian yellow cattle was detected by gas chromatograph, and the longissimus dorsi muscle was detected by real-time fluorescence quantitative PCR. The regulating factor related to lipid metabolism in subcutaneous adipose tissue, SREBPlcPPAR 纬 LXR 伪, and the key enzyme SCDCC-FASL LPLC C / EBP 伪, were found in subcutaneous adipose tissue. C- EBP 尾 -CPT1 尾, a related regulatory hormone, InsRN, GLNRN, TNFa, Interleukin-6. The expression of Adiponectin gene was detected by Western blot in subcutaneous adipose tissue. The results showed that adding 8% flax seeds to diet could increase n-3 PUFAs in fat tissue and muscle tissue of Yanbian cattle. The content of n-6 PUFAs decreased the content of saturated fatty acid (palmitic acid, stearic acid); (2) the increase of n3PUFAsn6PUFAs in adipose tissue could promote the transcription factor SREBP-1CnPPAR involved in lipid metabolism in the subcutaneous adipose tissue of Yanbian cattle. Y gene expression and protein expression level, and the effect of flaxseed fragmentation is more obvious, adipose tissue LXR 伪 gene expression level and protein expression is also related to flaxseed fragmentation treatment; (3) increasing the content of n-3 PUFAsN n-6 PUFAs in diet is beneficial to the increase of fatty acid synthesis key enzyme SCDD-FASA in adipose tissue. The effect of LPL on C / EBP 伪 and C / EBP 尾 was lower than that on the key enzyme (n-3 PUFAs). The increase of CPT1 in adipose tissue by n-6 PUFAs was higher than that in muscle tissue. 4) the addition of flaxseed could improve the gene expression of GHR-GLNR and cytokine Adiponectinine IL-6 TNF- 伪 in adipose tissue. In adipose tissue, the promoting effect was the most obvious. The expression of Ins R was opposite to that of GLNR. To sum up, adding 8% flax seeds to Yanbian yellow cattle diet was beneficial to increase C18: 1 in longissimus dorsi and subcutaneous adipose tissue. The content of C18: 2: C18: 2n-6: C18: 3n-3 decreases the content of C16: 0 and C18: 0. The effect of treated flaxseed was better. 2) the increase of n-3 PUFAs-n-6 PUFAs in adipose tissue. The expression of transcription factor SREBP-1 / PPARy gene and protein in subcutaneous adipose tissue of Yanbian cattle was up-regulated. The effect of crushed flaxseed was more obvious. 3) increasing the content of flaxseed in diet was beneficial to increase the fatty acid synthase related enzyme SCDX FASACCL (LPLL) in adipose tissue. The expression level of GHR-GLNR- TNF- 伪 and ADII-6) gene.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S823.5

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