黑木耳种质资源评价及查询系统的建立

发布时间：2018-03-31 10:56

本文选题：ITS序列　切入点：农艺性状　出处：《吉林农业大学》2017年硕士论文

【摘要】：本研究收集得到亚洲、北美洲和非洲等地区栽培及野生木耳菌株133株,初步建立木耳种质资源库模型。主要研究结果如下:1、菌株鉴定结果表明除菌株HYS02、HYS16、HYS20、HYS24、HYS27和HONG外的菌株均为Auricularia heimuer,菌株HYS20为A.villosula,菌株HYS16与A.heimuer和A.auricular-judae的亲缘关系均较近;菌株HYS02与A.heimuer的亲缘关系较近,菌株HYS24与Exidia recisa的亲缘关系较近。2、种质资源评价,结果表明菌落特性与农艺性状的相关性显著,菌落菌丝生长速度越快,耳片湿干比越低;菌丝分解木屑速度越快催芽率越高、耳片越重;菌落越浓密满袋时间越短;产生的气生菌丝越多催芽率越低、产量越低;菌丝分泌色素越多出耳周期越长、出耳不集中且转茬周期长;主成分分析中产量、出芽率、耳片数量、转化率等4个性状构成了14个农艺性状的第一个主成分,褶皱深度和褶皱数量构成了第2个主成分,百片重构成了第3个主成分,三个主成分的贡献率高达62.5%,“产量-耳片形态”可以作为农艺性状的评价指标。3、采用孢子保藏、继代保藏、木屑保藏和子实体保藏等保藏方法,结果表明滤纸条保藏优于孢子悬液保藏;子实体保藏在菌落形态、菌丝生长速度、分解木屑料能力和酶活性方面均优于继代保藏;菌落密度、漆酶和酸性蛋白酶活性与保藏方式相关性显著,可以作为保藏方式的评价指标,漆酶与菌丝分解木屑速度、出芽率和产量相关性显著,漆酶活性越高菌丝分解木屑速度越快、出芽率越高、产量越高;孢子萌发率最高的是无保护剂液氮保藏。4、黑木耳种质资源库模型的建立。依据以上研究结果,利用Microsoft Office Access2010软件初步建立黑木耳种质资源库模型,建立人机对话系统。研究者可以根据要求对菌株信息进行查询。
[Abstract]:In this study 133 strains of cultivated and wild Auricularia auricula strains were collected from Asia North America and Africa and the germplasm resource bank model of Auricularia auricula was established.The main results were as follows: 1. The results of strain identification showed that the strains with the exception of HYS02HYS16HYS20HYS24HYS27 and HONG were Auricularia heimuer, strain HYS20 was A. villosula. the relationship between HYS16 and A.heimuer and A.auricular-judae was close, and that between HYS02 and A.heimuer was close.The relationship between HYS24 and Exidia recisa was close to .2. the germplasm resources were evaluated. The results showed that the correlation between colony characteristics and agronomic characters was significant, the faster the growth rate of colony mycelium was, the lower the wet / dry ratio of ear piece was, and the faster the speed of decomposition of sawdust from mycelium was, the higher the germination rate was.The first principal component of 14 agronomic traits was composed of the number of ear slices and the transformation rate, the second principal component was composed of fold depth and fold number, and the third principal component was composed of 100 pieces weight.The contribution rate of the three principal components is as high as 62.5%. "yield-ear shape" can be used as the evaluation index of agronomic characters. The methods of spore preservation, subculture preservation, sawdust preservation and fruiting body preservation are adopted.The results showed that the preservation of filter paper strip was superior to that of spore suspension, and the preservation of fruiting body was superior to that of subculture preservation in colony morphology, mycelium growth rate, ability of decomposing sawdust and enzyme activity.Laccase and acid protease activity have a significant correlation with preservation mode. Laccase and mycelium decompose sawdust speed, bud rate and yield are significant correlation, the higher the laccase activity is, the faster the mycelium decomposes sawdust, and the higher the laccase activity is, the faster laccase activity can be used to decompose sawdust.The higher the sprouting rate, the higher the yield, the higher the spore germination rate, the higher the rate of spore germination is. 4. The establishment of Auricularia auricula germplasm resource bank model.Based on the above results, the germplasm resource database model of Auricularia auricula was established by using Microsoft Office Access2010 software, and the man-machine dialogue system was established.Researchers can query strain information as required.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S646.6

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