金线莲多糖对环磷酰胺所致免疫抑制小鼠脾脏细胞因子及转录因子mRNA表达的影响

发布时间：2018-03-10 09:30

本文选题：金线莲多糖　切入点：免疫抑制　出处：《中国兽医学报》2017年07期 　论文类型：期刊论文

【摘要】：50只雄性昆明小鼠适应性饲养1周后随机分为5组,分别为空白对照组、模型组及金线莲多糖(ARP)低、中、高剂量组,每组10只;第1~3天,除空白对照组注射生理盐水外,其他各组腹腔注射环磷酰胺(CTX)80mg/kg,连续3d;第4~10天,空白组和模型组灌服蒸馏水,其他组分别灌服100,200,400mg/kg的ARP。末次给药24h后,采集脾脏,qRT-PCR检测试验小鼠脾脏细胞因子(IL-2、IFN-γ、IL-4、IL-6)及转录因子(T-bet、GATA-3)mRNA表达。结果显示:与空白对照组相比,模型组IL-2、GATA-3 mRNA表达量降低,IL-4、IL-6、IFN-γ、T-bet mRNA表达量升高;与模型组相比,ARP高剂量组小鼠IL-4mRNA相对表达量降低(P0.05),中、高剂量组IL-6、IFN-γmRNA相对表达量降低(P0.05),ARP组T-bet的相对表达量降低(P0.05),高剂量组GATA-3的相对表达量升高(P0.05)。结果表明:ARP通过上调或下调小鼠脾脏细胞因子与转录因子的表达,调节免疫抑制小鼠的免疫功能。
[Abstract]:After one week of adaptive feeding, 50 male Kunming mice were randomly divided into 5 groups: blank control group, model group and AARP group with low, medium and high doses, 10 rats in each group. The other groups were intraperitoneally injected with CTX 80 mg / kg of cyclophosphamide for 3 days. On the 4th day, the blank group and the model group were given distilled water, and the other groups were given 100 ~ 200g / kg ARP of 400 mg / kg respectively. 24 hours after the last administration, The expression of IL-2mil and T-beta-GATA-3T mRNA were detected by qRT-PCR. The results showed that compared with the control group, the expression of IL-2tGATA-3 mRNA in the model group was significantly lower than that in the control group, and the expression of IL-4 IL-6IFN- 纬 T-bet mRNA in the model group was higher than that in the control group. Compared with the model group, the relative expression of IL-4mRNA in the high dose group was lower than that in the model group. The relative expression of T-bet was decreased in high dose group (P 0.05), and increased in high dose group (P 0.05). The results showed that the expression of spleen cytokines and transcription factors were up-regulated or down-regulated. To regulate the immune function of immunosuppressive mice.
【作者单位】：福建农林大学中西兽医结合与动物保健福建省高校重点实验室;
【基金】：国家自然科学基金资助项目(31372474)
【分类号】：S853.74

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