羊奇异变形杆菌的分离鉴定及药敏试验

发布时间：2018-03-10 10:32

本文选题：羔羊腹泻　切入点：奇异变形杆菌　出处：《动物医学进展》2017年07期 　论文类型：期刊论文

【摘要】：为查明宁夏地区某羊场羔羊腹泻的发病原因并选择敏感药物用于治疗,从患病羔羊粪便中分离出4株细菌,进行形态特征、培养特性、16S rRNA测序、VITEK微生物鉴定系统鉴定、致病性试验及药敏试验。结果显示,分离菌为奇异变形杆菌。动物试验表明,4株奇异变形杆菌对小鼠具有很强的致病性。药敏试验表明,4株奇异变形杆菌对氨基糖甙类抗生素(阿米卡星、卡那霉素、链霉素)、氟苯尼考及头孢菌素类抗生素敏感,而对大环内酯类(红霉素和克林霉素)、复方新诺明、恩诺沙星全部耐药。对青霉素类(哌拉西林、氨苄西林、阿莫西林)、喹诺酮类(环丙沙星、氧氟沙星、诺氟沙星)、四环素、万古霉素、庆大霉素呈现不同程度的耐药。结果表明,该4株奇异变形杆菌具有较强的致病力和多重耐药性,可能是此次羔羊腹泻的致病菌,治疗首选氨基糖甙类、氟苯尼考及头孢菌素类抗生素。
[Abstract]:In order to find out the cause of lamb diarrhea in a sheep farm in Ningxia area and to select sensitive drugs for treatment, four strains of bacteria were isolated from the feces of sick lambs, and their morphological characteristics and culture characteristics were identified by 16s rRNA sequencing and VITEK microbiological identification system. Pathogenicity tests and drug susceptibility tests. The results show that, The isolates were Proteus mirabilis. Animal tests showed that 4 strains of Proteus mirabilis had strong pathogenicity to mice. Drug sensitivity test showed that 4 strains of Proteus mirabilis were p-aminoglycoside antibiotics (amikacin, kanamycin, kanamycin). Streptomycin, florfenicol and cephalosporins are sensitive to macrolides (erythromycin and clindamycin, compound sulfamine, enrofloxacin), penicillin (piperacillin, ampicillin), Amoxicillin, quinolones (ciprofloxacin, ofloxacin, norfloxacin, tetracycline, vancomycin, gentamicin) showed different degrees of drug resistance. The results showed that the four strains of Proteus mirabilis had strong pathogenicity and multidrug resistance. May be the pathogenic bacteria of lamb diarrhea, the first choice of treatment of aminoglycosides, florfenicol and cephalosporins antibiotics.
【作者单位】：宁夏农林科学院动物科学研究所;宁夏大学新华学院;
【基金】：宁夏农林科学院先导基金项目(NKYJ-14-10)
【分类号】：S858.26

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