马红球菌菌落印迹检测方法的建立

发布时间：2018-03-10 15:00

本文选题：马红球菌　切入点：菌落印记法　出处：《中国预防兽医学报》2017年04期 　论文类型：期刊论文

【摘要】：为建立快速检测环境中马红球菌(R.equi)的方法,本研究以非致病性R.equi ATCC6939以及致病性R.equi毒力相关脂蛋白A(Vap A)分别免疫家兔,制备了兔抗非致病性R.equi多抗与兔抗致病性R.equi Vap A蛋白多抗,并以其作为检测抗体,通过优化反应条件,建立了R.equi菌落印迹检测方法。结果显示该方法可特异性检测R.equi并可区分致病性与非致病性R.equi;其R.equi检测敏感性高,特异性强,均明显优于R.equi的PCR鉴定法。利用本研究建立的方法对全国部分地区马场土壤样本的初步调查表明R.equi在部分地区马场土壤中普遍存在,然而未检测到致病性R.equi。本实验建立的检测方法可应用于我国马场环境中R.equi的流行病学调查,为我国马病防控平台提供有力的技术支持,并为中国现代育马场的建设提供必要的参考。
[Abstract]:In order to establish a rapid method for the detection of R. equi in environment, rabbits were immunized with non-pathogenic R.equi ATCC6939 and virulence associated lipoprotein (ATCC6939) respectively. The anti-nonpathogenic polyantibodies of R. equi and the polyantibodies of anti-pathogenic Vap A protein were prepared. The results showed that the method could be used to detect R.equi specifically and distinguish the pathogenicity and non-pathogenicity of R. equi, and the sensitivity and specificity of R. equi were high. The preliminary investigation on the soil samples of racetrack in some parts of the country showed that R. equi was widely found in the soil of some areas of the country, and the results showed that R. equi was more common than R. equi's method in the identification of racetrack soil in some parts of the country. However, no pathogenicity R. equi. the method established in this experiment can be applied to the epidemiological investigation of R. equi in the racetrack environment of our country, and provide strong technical support for the equine disease prevention and control platform in China. It also provides the necessary reference for the construction of modern horse breeding farm in China.
【作者单位】：华南农业大学兽医学院;广东海大畜牧兽医研究院;广东出入境检验检疫局技术中心;广东省兽医临床重大疾病综合防控重点实验室;
【基金】：国家自然科学基金(31402259) 广东省科技计划项目(2013B040200032) 广东省自然科学基金(S2013040015745) 广东省兽医临床重大疾病综合防控重点实验室(2013A061401013) 国家质检总局科研项目(2012IK006)
【分类号】：S852.61

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