脂多糖和玉米赤霉烯酮介导的内质网应激对山羊子宫内膜基质细胞的作用

发布时间：2024-12-26 04:23

　　繁殖障碍,特别是怀孕早期胚胎丢失会造成严重的经济损失。导致早期胚胎丢失的原因很多,如环境中的细菌感染和玉米赤霉烯酮污染。脂多糖(LPS)是革兰式阴性菌的主要毒力因子,是由脂质和由O-抗原组成的多糖组成的大分子,外核和内核通过共价键连接。有证据表明LPS导致家畜流产。玉米赤霉烯酮(ZEA)是由一些镰刀菌属和赤霉属物种产生的一种的雌激素类似物,具有热稳定性,并且在世界范围内的许多谷类作物中发现了赤霉烯酮,例如玉米,大麦,燕麦,小麦,水稻和高粱。玉米赤霉烯酮已经被证明是主要的毒素,会导致不育、流产或其他繁殖问题。进一步揭示和分析LPS和ZEA对子宫内膜细胞作用的机制具有重要意义。据报道,有几个因素在胚胎着床阶段起着关键作用,包括激素、血管活性基因、细胞因子、生长因子和环境因素。然而,在胚胎植入过程中,胚胎植入前的发育和胚胎-子宫相互作用的分子途径仍不清楚。在本研究中,我们关注植入阶段并研究脂质多糖和玉米赤霉烯酮感染的山羊子宫内膜基质细胞(GESCs)的病理生理变化。LPS和/或ZEA用作影响细胞氧化应激的模型。1.内质网应激参与脂多糖诱导的山羊子宫内膜基质细胞的炎症反应和细胞凋亡内质网应激(E...

【文章页数】：64 页

【学位级别】：博士

【文章目录】：
ABSTRACT
摘要
Review and Literature
    Chapter Ⅰ:The ER stress and Effects of Lipopolysaccharide and Zearalenone
        1.Endoplasmic reticulum stress
        2.Pre-implantation embryos development
        3.Lipopolysaccharide and/or Zearalenone induced cell apoptosis
        4.Lipopolysaccharide and/or Zearalenone induced cell proliferation
        5.Lipopolysaccharide and/or Zearalenone induced autophagy
        6.Lipopolysaccharide and/or Zearalenone induced activation of ER stress
        7.Lipopolysaccharide and/or Zearalenone induced inflammatory cytokines
        8.Some ways to blocked endoplasmic reticulum stress
Experimental research
    Chapter Ⅱ:Endoplasmic Reticulum Stress is Involved in Lipopolysaccharide Induced Inflammatory Response and Apoptosis in Goat Endometrial Stromal Cells
        1.MATERIALS AND METHODS
            1.1 Cell culture and drug treatment
            1.2 Cell viability
            1.3 Apoptosis analysis
            1.4 Total RNA extraction and real-time quantitative PCR
            1.5 ELISA
            1.6 Western blot analysis
            1.7 Knock down ATF6 and IRE1 genes with small interfering RNA
            1.8 Statistical analysis
        2.RESULTS
            2.1 Functional role of LPS on ESC cell proliferation
            2.2 Effect of LPS on ESC apoptosis and PCNA,MKI67 expression
            2.3 LPS activated Endoplasmic reticulum stress
            2.4 LPS affected on expression of autophagy and mitochondria-related genes
            2.5 LPS increased inflammatory cytokines secretion
            2.6 4-phenylbutyrate inhibited the expression of ER stress protein,autophagy and pro-inflammatory cytokines under LPS treatment
            2.7 Effect of ATF6 and IRE1 on promoting autophagy
        DISCUSSION
    Chapter Ⅲ:Endoplasmic Reticulum Stress is Involved in Mycotoxin Zearalenone Induced Inflammatory Response,Proliferation,and Apoptosis in Goat Endometrial Stromal Cells
        1.Materials and methods
            1.1 Chemicals
            1.2 Cell culture and treatments
            1.3 Cell proliferation
            1.4 Cell apoptosis assay
            1.5 ELISA
            1.6 Western blot
            1.7 Knockdown ERN1 gene by small interfering RNA
            1.8 Statistical analysis
        2.Results
            2.1 Dynamic role of ZEA on ESC proliferation and apoptosis assay
            2.2 Endoplasmic reticulum stress-activated by ZEA on Goat endometrial stromal cells
            2.3 Autophagy and inflammatory cytokine affected with ZEA on ESCs
            2.4 knockdown IRE1 and affected on promoting autophagy and inflammation
        DISCUSSION
Conclusion
General Conclusion
REFERENCES
Acknowledgements
作者简介



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