新生乳鼠脑缺血缺氧模型制作及评价的实验研究

发布时间：2017-12-27 10:01

本文关键词：新生乳鼠脑缺血缺氧模型制作及评价的实验研究　出处：《新疆医科大学》2008年硕士论文　论文类型：学位论文

【摘要】： 目的探讨新生乳鼠缺血缺氧脑瘫模型的制作及评价方法。方法选用出生7天的健康Wistar幼鼠,结扎右侧颈总动脉结合8%氧气、体积分数92%氮气环境缺氧的方法制作幼鼠脑瘫模型,不同时间点对实验动物进行平衡木试验、足印分析、运动诱发电位及病理学检测。结果(1)足印重复间距试验结果显示HIE组术后3周、7周时实验鼠左侧前后肢足印重复性差。表现为间距较大,且间距不稳定,明显大于空白对照组(P＜0.05);(2)术后3周、7周平衡木试验结果显示潜伏期及后足滑脱次数上HIE组明显多于对照组(P＜0.05);(3)术后3周、7周时HIE组实验鼠后肢股四头肌复合动作电位(CMAP)与对照组相比引出波幅明显较低(P＜0.05);(4)术后3周,大体观察右侧脑组织体积变小,萎缩。HE染色光镜观察损伤侧脑室周围细胞肿胀,细胞数量较对侧少;损伤侧大脑皮层神经细胞明显减少,神经细胞变性坏死,胶质细胞反应性增生。结论应用一侧颈总动脉结合缺氧的方法制作幼鼠脑瘫模型稳定、可靠,具有进一步的推广意义,并可用于脑瘫的相关实验研究。神经行为学及神经电生理在脑瘫动物模型的评价方面有很大应用空间。
[Abstract]:Objective to explore the method of making and evaluating the model of ischemic hypoxic cerebral palsy in neonatal rats. Methods 7 days old healthy Wistar rats, right carotid artery ligation produced cerebral palsy model combined with 8% oxygen, 92% volume fraction of nitrogen oxygen, at different time points of balance beam test, detection of experimental animal footprints analysis, motor evoked potentials and pathology. Results (1) the results of repeat interval test showed that the left hind limbs of the HIE group had poor reproducibility at the left and the left hind limbs at 3 and 7 weeks after operation. For larger spacing and the spacing is not stable, significantly greater than the control group (P < 0.05); (2) after 3 weeks, 7 weeks, balance beam test results show that the latency and the number of foot slippage in HIE group was higher than that of control group (P < 0.05); (3) 3 weeks after surgery, 7 week HIE group experimental rat femoral head four muscle compound action potential (CMAP) compared with the control group leads to amplitude was significantly lower (P < 0.05); (4) 3 weeks after operation, gross observation of the right side of the brain tissue volume, atrophy. HE staining and light microscopy showed that the cells in the injured side ventricles were swollen, the number of cells was less than that on the contralateral side, the neurons in the injured side were significantly reduced, the degeneration and necrosis of neurons, and the reactive proliferation of glial cells. Conclusion it is stable and reliable to use unilateral common carotid artery combined with anoxia to make cerebral palsy model in young rats, which has further popularization significance and can be used for experimental study of cerebral palsy. Neurobehavioral and neuroelectrophysiology are very useful in the evaluation of cerebral palsy animal models.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R722.121;R-332

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