一种新的抗病毒分子HIRRP的相互作用蛋白筛

发布时间：2017-12-28 22:27

本文关键词：一种新的抗病毒分子HIRRP的相互作用蛋白筛选、验证及生物学功能分析　出处：《中国细胞生物学学报》2010年06期 　论文类型：期刊论文

【摘要】：单纯疱疹病毒1型感染相关抑制蛋白(HSV-1 infection related repress protein,HIRRP)是一个结构和功能仍未知的蛋白。我们前期的工作发现HIRRP能在病毒感染的细胞内高表达,表明HIRRP可能在细胞内参与了抗病毒感染这一重要的生物学事件。然而,HIRRP在细胞内参与抗病毒感染的具体作用机理仍不清楚。为此,我们以HIRRP为诱饵构建了pGBKT7-HIRRP载体,用酵母双杂交技术从人肝细胞cDNA文库中筛选出了玻连蛋白(Vitronectin,VTN),并通过体内免疫共沉淀和双荧光共定位实验,以及体外的促细胞增殖实验和病毒入胞干预实验,证实了HIRRP和VTN之间存在着相互作用。初步的功能分析显示,两者的相互作用,具有协同促进细胞抗病毒的效应。由此,我们得出结论,HIRRP和VTN在细胞内能发生相互作用,并猜测这种作用与细胞的抗病毒感染活动相关。
[Abstract]:Herpes simplex virus type 1 infection related inhibitor protein (HSV-1 infection related repress protein, HIRRP) is a protein that is still unknown in structure and function. Our previous work found that HIRRP could be highly expressed in viral infected cells, suggesting that HIRRP may play an important part in the biological events of viral infection in cells. However, the specific mechanism of HIRRP involvement in antiviral infection is still unclear. To this end, we use HIRRP as bait vector pGBKT7-HIRRP was constructed by using yeast two hybrid technique from human liver cell cDNA library screened vitronectin (Vitronectin, VTN), and co precipitation by in vivo immune and fluorescence co localization, and cell proliferation experiments and viropexis intervention experiment in vitro, confirmed the interaction exists between HIRRP and VTN. Preliminary functional analysis shows that the interaction of the two has synergistic effects on promoting cell resistance. As a result, we conclude that HIRRP and VTN interact in cells within the cell, and that this effect is associated with the activity of the cell's antiviral infection.
【作者单位】：北京协和医学院中国医学科学院医学生物学研究所;
【分类号】：R373
【正文快照】： 单纯疤疹病毒1型感染相关抑制蛋白(H sv一lin-feetion related rePress Protein，HIRRP)(Aeeession No.BAB71400)最早由日本科学家通过对人的cDNA测序而鉴别到111。HIRRP包含209个氨基酸残基，，理论预测相对分子质量约24.6kDa，理论等电点为7.94。目前，还没有任何有关该蛋白

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